Surface Modification with NGF-Loaded Chitosan/Heparin Nanoparticles for Improving Biocompatibility of Cardiovascular Stent
Late thrombosis and restenosis remain major challenges to the safety of drug-eluting stents. Biofunctional modification to endow the surface with selective anticoagulation and promote endothelium regeneration has become a hotpot recently. In this study, chitosan and heparin were found to form three-...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/9941143 |
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| _version_ | 1832555207902363648 |
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| author | Haixin Song Tao Wu Xiaotian Yang Yangzheng Li Ye Ye Bo Li Tao Liu Shihui Liu Jianhua Li |
| author_facet | Haixin Song Tao Wu Xiaotian Yang Yangzheng Li Ye Ye Bo Li Tao Liu Shihui Liu Jianhua Li |
| author_sort | Haixin Song |
| collection | DOAJ |
| description | Late thrombosis and restenosis remain major challenges to the safety of drug-eluting stents. Biofunctional modification to endow the surface with selective anticoagulation and promote endothelium regeneration has become a hotpot recently. In this study, chitosan and heparin were found to form three-dimensional nanoparticles by spontaneous electrostatic interaction. Based on the specific binding properties between heparin and nerve growth factor (NGF), a new type of NGF-loaded heparin/chitosan nanoparticles was constructed for surface modification. The results of material characterization show that the nanoparticles are successfully immobilized on the surface of the material. In vitro blood compatibility and endothelial cell compatibility assay showed that the modified surface could selectively inhibit platelet adhesion and smooth muscle cell overproliferation, while accelerating endothelialization via promoting endothelial cell proliferation and enhancing endothelial progenitor cell mobilization. |
| format | Article |
| id | doaj-art-d657d32386f54aa280e1a3cf89e5666a |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-d657d32386f54aa280e1a3cf89e5666a2025-02-03T05:49:26ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/99411439941143Surface Modification with NGF-Loaded Chitosan/Heparin Nanoparticles for Improving Biocompatibility of Cardiovascular StentHaixin Song0Tao Wu1Xiaotian Yang2Yangzheng Li3Ye Ye4Bo Li5Tao Liu6Shihui Liu7Jianhua Li8Department of Rehabilitation, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, 310000 Zhejiang Province, ChinaDepartment of Rehabilitation, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, 310000 Zhejiang Province, ChinaDepartment of Rehabilitation, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, 310000 Zhejiang Province, ChinaDepartment of Rehabilitation, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, 310000 Zhejiang Province, ChinaDepartment of Rehabilitation, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, 310000 Zhejiang Province, ChinaDepartment of Rehabilitation, Tongde Hospital of Zhejiang Province, Hangzhou, 310000 Zhejiang Province, ChinaMedical College of Acu-Moxi and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou, 510006 Guangdong Province, ChinaMedical College of Acu-Moxi and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou, 510006 Guangdong Province, ChinaDepartment of Rehabilitation, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, 310000 Zhejiang Province, ChinaLate thrombosis and restenosis remain major challenges to the safety of drug-eluting stents. Biofunctional modification to endow the surface with selective anticoagulation and promote endothelium regeneration has become a hotpot recently. In this study, chitosan and heparin were found to form three-dimensional nanoparticles by spontaneous electrostatic interaction. Based on the specific binding properties between heparin and nerve growth factor (NGF), a new type of NGF-loaded heparin/chitosan nanoparticles was constructed for surface modification. The results of material characterization show that the nanoparticles are successfully immobilized on the surface of the material. In vitro blood compatibility and endothelial cell compatibility assay showed that the modified surface could selectively inhibit platelet adhesion and smooth muscle cell overproliferation, while accelerating endothelialization via promoting endothelial cell proliferation and enhancing endothelial progenitor cell mobilization.http://dx.doi.org/10.1155/2021/9941143 |
| spellingShingle | Haixin Song Tao Wu Xiaotian Yang Yangzheng Li Ye Ye Bo Li Tao Liu Shihui Liu Jianhua Li Surface Modification with NGF-Loaded Chitosan/Heparin Nanoparticles for Improving Biocompatibility of Cardiovascular Stent Stem Cells International |
| title | Surface Modification with NGF-Loaded Chitosan/Heparin Nanoparticles for Improving Biocompatibility of Cardiovascular Stent |
| title_full | Surface Modification with NGF-Loaded Chitosan/Heparin Nanoparticles for Improving Biocompatibility of Cardiovascular Stent |
| title_fullStr | Surface Modification with NGF-Loaded Chitosan/Heparin Nanoparticles for Improving Biocompatibility of Cardiovascular Stent |
| title_full_unstemmed | Surface Modification with NGF-Loaded Chitosan/Heparin Nanoparticles for Improving Biocompatibility of Cardiovascular Stent |
| title_short | Surface Modification with NGF-Loaded Chitosan/Heparin Nanoparticles for Improving Biocompatibility of Cardiovascular Stent |
| title_sort | surface modification with ngf loaded chitosan heparin nanoparticles for improving biocompatibility of cardiovascular stent |
| url | http://dx.doi.org/10.1155/2021/9941143 |
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