Alpha B-crystallin Ameliorates Imbalance of Redox Homeostasis, Inflammation and Apoptosis in an Acute Lung Injury Model with Rats
Objective: Ischemia-reperfusion (IR) of the aorta is a significant contributor to the development of postoperative acute lung damage after abdominal aortic surgery. The aim of the present study was to examine the effect of alpha B-crystallin, a small heat shock protein (known as HspB5), on lung inju...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2024-09-01
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| Series: | Medeniyet Medical Journal |
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| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=medeniyet&un=MEDJ-82274 |
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| author | Seda KOCAK İbrahim GUNER Muhittin Onur YAMAN Tugba EKIZ YILMAZ Emine Elif GUZEL MEYDANLI Nermin YELMEN Gulderen SAHIN |
| author_facet | Seda KOCAK İbrahim GUNER Muhittin Onur YAMAN Tugba EKIZ YILMAZ Emine Elif GUZEL MEYDANLI Nermin YELMEN Gulderen SAHIN |
| author_sort | Seda KOCAK |
| collection | DOAJ |
| description | Objective: Ischemia-reperfusion (IR) of the aorta is a significant contributor to the development of postoperative acute lung damage after abdominal aortic surgery. The aim of the present study was to examine the effect of alpha B-crystallin, a small heat shock protein (known as HspB5), on lung injury induced by abdominal aortic IR in rats.
Methods: Male Sprague-Dawley rats were divided into three groups: control, ischemia-reperfusion (IR, 90 min ischemia and 180 min reperfusion), and alpha B-crystallin +IR. Alpha B-crystallin (50 μg/100 g) was intraperitoneally administered 1 h before IR. Lung tissue samples were obtained for histological and biochemical analyses of oxidative stress and cytokine and apoptosis parameters in plasma, lung tissues, and bronchoalveolar lavage (BAL) fluid.
Results: The levels of malondialdehyde, reactive oxygen species, total oxidant status, tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), nuclear factor kappa B (NFKβ), caspase-9 (CASP-9), 8-hydroxy-2'-deoxyguanosine, total antioxidant status, superoxide dismutase, and interleukin-10 levels in lung tissues, plasma, and BAL fluid (p<0.05 versus control) increased in Aortic IR. However, alpha B-crystallin significantly reduced the lung tissue levels of oxidative, inflamatuvar, and apoptotic parameters in the plasma, lung tissues, and BAL fluid (p<0.05 versus aortic IR). Histopathological results showed that alpha B-crystallin ameliorated the morphological changes related to lung injury (p<0.001).
Conclusion: Alpha B-crystallin substantially restored disrupted the redox balance, inflammation, and apoptotic parameters in rats exposed to IR. The cytoprotective effect of alpha B-crystallin on redox balance might be attributed to improved lung injury. |
| format | Article |
| id | doaj-art-d5f51a8260cb4148bff1f4e8cb9db4ca |
| institution | Kabale University |
| issn | 2149-2042 2149-4606 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Medeniyet Medical Journal |
| spelling | doaj-art-d5f51a8260cb4148bff1f4e8cb9db4ca2025-01-29T10:22:46ZengGalenos Publishing HouseMedeniyet Medical Journal2149-20422149-46062024-09-0139321122010.4274/MMJ.galenos.2024.82274MEDJ-82274Alpha B-crystallin Ameliorates Imbalance of Redox Homeostasis, Inflammation and Apoptosis in an Acute Lung Injury Model with RatsSeda KOCAK0İbrahim GUNER1Muhittin Onur YAMAN2Tugba EKIZ YILMAZ3Emine Elif GUZEL MEYDANLI4Nermin YELMEN5Gulderen SAHIN6Kirsehir Ahi Evran University Faculty of Medicine, Department of Physiology, Kirsehir, TürkiyeTekirdag Namik Kemal University Faculty of Medicine, Department of Physiology, Tekirdag, TürkiyeIstanbul University-Cerrahpasa, Cerrahpasa Faculty of Medicine, Department of Physiology, Istanbul, TürkiyeIstanbul University-Cerrahpasa, Cerrahpasa Faculty of Medicine, Department of Histology and Embriology, Istanbul, TürkiyeIstanbul University-Cerrahpasa, Cerrahpasa Faculty of Medicine, Department of Histology and Embriology, Istanbul, TürkiyeIstanbul University-Cerrahpasa, Cerrahpasa Faculty of Medicine, Department of Physiology, Istanbul, TürkiyeIstanbul Aydin University Faculty of Medicine, Department of Physiology, Istanbul, TürkiyeObjective: Ischemia-reperfusion (IR) of the aorta is a significant contributor to the development of postoperative acute lung damage after abdominal aortic surgery. The aim of the present study was to examine the effect of alpha B-crystallin, a small heat shock protein (known as HspB5), on lung injury induced by abdominal aortic IR in rats. Methods: Male Sprague-Dawley rats were divided into three groups: control, ischemia-reperfusion (IR, 90 min ischemia and 180 min reperfusion), and alpha B-crystallin +IR. Alpha B-crystallin (50 μg/100 g) was intraperitoneally administered 1 h before IR. Lung tissue samples were obtained for histological and biochemical analyses of oxidative stress and cytokine and apoptosis parameters in plasma, lung tissues, and bronchoalveolar lavage (BAL) fluid. Results: The levels of malondialdehyde, reactive oxygen species, total oxidant status, tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), nuclear factor kappa B (NFKβ), caspase-9 (CASP-9), 8-hydroxy-2'-deoxyguanosine, total antioxidant status, superoxide dismutase, and interleukin-10 levels in lung tissues, plasma, and BAL fluid (p<0.05 versus control) increased in Aortic IR. However, alpha B-crystallin significantly reduced the lung tissue levels of oxidative, inflamatuvar, and apoptotic parameters in the plasma, lung tissues, and BAL fluid (p<0.05 versus aortic IR). Histopathological results showed that alpha B-crystallin ameliorated the morphological changes related to lung injury (p<0.001). Conclusion: Alpha B-crystallin substantially restored disrupted the redox balance, inflammation, and apoptotic parameters in rats exposed to IR. The cytoprotective effect of alpha B-crystallin on redox balance might be attributed to improved lung injury.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=medeniyet&un=MEDJ-82274abdominal surgerycytokinesinflammationimmunologyischemia-reperfusion |
| spellingShingle | Seda KOCAK İbrahim GUNER Muhittin Onur YAMAN Tugba EKIZ YILMAZ Emine Elif GUZEL MEYDANLI Nermin YELMEN Gulderen SAHIN Alpha B-crystallin Ameliorates Imbalance of Redox Homeostasis, Inflammation and Apoptosis in an Acute Lung Injury Model with Rats Medeniyet Medical Journal abdominal surgery cytokines inflammation immunology ischemia-reperfusion |
| title | Alpha B-crystallin Ameliorates Imbalance of Redox Homeostasis, Inflammation and Apoptosis in an Acute Lung Injury Model with Rats |
| title_full | Alpha B-crystallin Ameliorates Imbalance of Redox Homeostasis, Inflammation and Apoptosis in an Acute Lung Injury Model with Rats |
| title_fullStr | Alpha B-crystallin Ameliorates Imbalance of Redox Homeostasis, Inflammation and Apoptosis in an Acute Lung Injury Model with Rats |
| title_full_unstemmed | Alpha B-crystallin Ameliorates Imbalance of Redox Homeostasis, Inflammation and Apoptosis in an Acute Lung Injury Model with Rats |
| title_short | Alpha B-crystallin Ameliorates Imbalance of Redox Homeostasis, Inflammation and Apoptosis in an Acute Lung Injury Model with Rats |
| title_sort | alpha b crystallin ameliorates imbalance of redox homeostasis inflammation and apoptosis in an acute lung injury model with rats |
| topic | abdominal surgery cytokines inflammation immunology ischemia-reperfusion |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=medeniyet&un=MEDJ-82274 |
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