Effects of synergistic fermentation of tea bee pollen with bacteria and enzymes on growth and intestinal health of Apis cerana cerana
While the health benefits of lactic acid bacteria (LAB)-fermented feed on farmed animals are well-established, its potential benefits for honeybees, specifically Apis cerana cerana, remain largely unexplored. The present study aimed to optimize an enzymatic hydrolysis process for tea bee pollen, emp...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Current Research in Microbial Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666517425000057 |
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| author | Panpan Xue Le Xu Yakai Tian Mingkui Lv Pingping Fang Kun Dong Qiuye Lin Zhenhui Cao |
| author_facet | Panpan Xue Le Xu Yakai Tian Mingkui Lv Pingping Fang Kun Dong Qiuye Lin Zhenhui Cao |
| author_sort | Panpan Xue |
| collection | DOAJ |
| description | While the health benefits of lactic acid bacteria (LAB)-fermented feed on farmed animals are well-established, its potential benefits for honeybees, specifically Apis cerana cerana, remain largely unexplored. The present study aimed to optimize an enzymatic hydrolysis process for tea bee pollen, employing a complex enzyme comprising acid cellulase and pectinase, followed by fermentation with Limosilactobacillus reuteri LP4. A. c. cerana workers were subsequently fed tea bee pollen processed with this optimized method. Under the optimal processing condition of fermented tea bee pollen, the pH value was 4.41, the protein content was 27.75 %, and the viable count of LAB was 2.31×10⁹ CFU/g. No molds and yeasts as well as pathogens were detected. Compared to the unfermented pollen, honey bee workers administrated with fermented tea pollen with L. reuteri LP4 showed significantly increased survival rate by 24.34 % on day 15. Moreover, the relative abundances of Lactobacillus and Bifidobacterium were elevated, while those of Enterococcus and Bacteroides were diminished. Concurrently, the relative expression levels of immune-related genes including Abaecin, PPO, Defensin, and Vg were significantly upregulated. These findings provide a scientific foundation for application of fermented feeds to enhance the health of A. c. cerana populations and contribute to the sustainable development of apiculture in China. |
| format | Article |
| id | doaj-art-d53cec1a2b02452a9676d662cf68d83f |
| institution | Kabale University |
| issn | 2666-5174 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Microbial Sciences |
| spelling | doaj-art-d53cec1a2b02452a9676d662cf68d83f2025-01-23T05:27:48ZengElsevierCurrent Research in Microbial Sciences2666-51742025-01-018100343Effects of synergistic fermentation of tea bee pollen with bacteria and enzymes on growth and intestinal health of Apis cerana ceranaPanpan Xue0Le Xu1Yakai Tian2Mingkui Lv3Pingping Fang4Kun Dong5Qiuye Lin6Zhenhui Cao7Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, PR ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, PR China; Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Kunming 650201, PR ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, PR ChinaSericulture and Apiculture Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi 661101, PR ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, PR ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, PR ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, PR China; Corresponding authors.Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, PR China; Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Kunming 650201, PR China; Corresponding authors.While the health benefits of lactic acid bacteria (LAB)-fermented feed on farmed animals are well-established, its potential benefits for honeybees, specifically Apis cerana cerana, remain largely unexplored. The present study aimed to optimize an enzymatic hydrolysis process for tea bee pollen, employing a complex enzyme comprising acid cellulase and pectinase, followed by fermentation with Limosilactobacillus reuteri LP4. A. c. cerana workers were subsequently fed tea bee pollen processed with this optimized method. Under the optimal processing condition of fermented tea bee pollen, the pH value was 4.41, the protein content was 27.75 %, and the viable count of LAB was 2.31×10⁹ CFU/g. No molds and yeasts as well as pathogens were detected. Compared to the unfermented pollen, honey bee workers administrated with fermented tea pollen with L. reuteri LP4 showed significantly increased survival rate by 24.34 % on day 15. Moreover, the relative abundances of Lactobacillus and Bifidobacterium were elevated, while those of Enterococcus and Bacteroides were diminished. Concurrently, the relative expression levels of immune-related genes including Abaecin, PPO, Defensin, and Vg were significantly upregulated. These findings provide a scientific foundation for application of fermented feeds to enhance the health of A. c. cerana populations and contribute to the sustainable development of apiculture in China.http://www.sciencedirect.com/science/article/pii/S2666517425000057Limosilactobacillus reuteri LP4Apis cerana ceranaFermented tea bee pollenSurvivalIntestine health |
| spellingShingle | Panpan Xue Le Xu Yakai Tian Mingkui Lv Pingping Fang Kun Dong Qiuye Lin Zhenhui Cao Effects of synergistic fermentation of tea bee pollen with bacteria and enzymes on growth and intestinal health of Apis cerana cerana Current Research in Microbial Sciences Limosilactobacillus reuteri LP4 Apis cerana cerana Fermented tea bee pollen Survival Intestine health |
| title | Effects of synergistic fermentation of tea bee pollen with bacteria and enzymes on growth and intestinal health of Apis cerana cerana |
| title_full | Effects of synergistic fermentation of tea bee pollen with bacteria and enzymes on growth and intestinal health of Apis cerana cerana |
| title_fullStr | Effects of synergistic fermentation of tea bee pollen with bacteria and enzymes on growth and intestinal health of Apis cerana cerana |
| title_full_unstemmed | Effects of synergistic fermentation of tea bee pollen with bacteria and enzymes on growth and intestinal health of Apis cerana cerana |
| title_short | Effects of synergistic fermentation of tea bee pollen with bacteria and enzymes on growth and intestinal health of Apis cerana cerana |
| title_sort | effects of synergistic fermentation of tea bee pollen with bacteria and enzymes on growth and intestinal health of apis cerana cerana |
| topic | Limosilactobacillus reuteri LP4 Apis cerana cerana Fermented tea bee pollen Survival Intestine health |
| url | http://www.sciencedirect.com/science/article/pii/S2666517425000057 |
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