Comparative Study of Lipid Profile for Mice Treated with Cyclophosphamide by HPLC-HRMS and Bioinformatics
<b>Purpose:</b> Immunodeficiency conditions, which are characterized by reduced immune activity that promotes the development of chronic diseases, are needed for efficient monitoring. A promising area of monitoring and early diagnosis of immunodeficiency diseases is the determination of...
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2025-01-01
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| author | Ekaterina Demicheva Fernando Jonathan Polanco Espino Pavel Vedeneev Vadim Shevyrin Aleksey Buhler Elena Mukhlynina Olga Berdiugina Alicia del Carmen Mondragon Alberto Cepeda Sáez Aroa Lopez-Santamarina Alejandra Cardelle-Cobas Olga Solovyova Irina Danilova Jose Manuel Miranda Elena Kovaleva |
| author_facet | Ekaterina Demicheva Fernando Jonathan Polanco Espino Pavel Vedeneev Vadim Shevyrin Aleksey Buhler Elena Mukhlynina Olga Berdiugina Alicia del Carmen Mondragon Alberto Cepeda Sáez Aroa Lopez-Santamarina Alejandra Cardelle-Cobas Olga Solovyova Irina Danilova Jose Manuel Miranda Elena Kovaleva |
| author_sort | Ekaterina Demicheva |
| collection | DOAJ |
| description | <b>Purpose:</b> Immunodeficiency conditions, which are characterized by reduced immune activity that promotes the development of chronic diseases, are needed for efficient monitoring. A promising area of monitoring and early diagnosis of immunodeficiency diseases is the determination of metabolic biomarkers in the blood. <b>Methods:</b> In this work, we identified a set of lipid biomarkers of immunodeficiency states by performing high-performance liquid chromatography–high-resolution mass spectrometry (HPLC-HRMS) analysis of blood plasma samples from mice and processing them with bioinformatics approaches. Potential biomarkers were selected through statistical analysis and further validated by MS/MS. <b>Conclusions:</b> As a result, 15 lipids were confirmed and selected as potential biomarkers of immunodeficiency states. The selected biomarkers can be further studied and serve as promising targets for the early diagnosis of immunodeficiency diseases. |
| format | Article |
| id | doaj-art-d515f50e0d2e449092eb777d557f5e7d |
| institution | Kabale University |
| issn | 2218-1989 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj-art-d515f50e0d2e449092eb777d557f5e7d2025-01-24T13:41:19ZengMDPI AGMetabolites2218-19892025-01-011516010.3390/metabo15010060Comparative Study of Lipid Profile for Mice Treated with Cyclophosphamide by HPLC-HRMS and BioinformaticsEkaterina Demicheva0Fernando Jonathan Polanco Espino1Pavel Vedeneev2Vadim Shevyrin3Aleksey Buhler4Elena Mukhlynina5Olga Berdiugina6Alicia del Carmen Mondragon7Alberto Cepeda Sáez8Aroa Lopez-Santamarina9Alejandra Cardelle-Cobas10Olga Solovyova11Irina Danilova12Jose Manuel Miranda13Elena Kovaleva14Institute of Natural Sciences and Mathematics, Ural Federal University, Ekaterinburg 620075, RussiaInstitute of Natural Sciences and Mathematics, Ural Federal University, Ekaterinburg 620075, RussiaInstitute of Chemical Engineering, Ural Federal University, Ekaterinburg 620002, RussiaInstitute of Chemical Engineering, Ural Federal University, Ekaterinburg 620002, RussiaInstitute of Natural Sciences and Mathematics, Ural Federal University, Ekaterinburg 620075, RussiaInstitute of Natural Sciences and Mathematics, Ural Federal University, Ekaterinburg 620075, RussiaInstitute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences, Ekaterinburg 620049, RussiaLaboratorio de Higiene, Inspección y Control de Alimentos, Departamento de Química Analítica, Nutrición y Bromatología, Campus Terra, Universidade da Santiago de Compostela, 27002 Lugo, SpainLaboratorio de Higiene, Inspección y Control de Alimentos, Departamento de Química Analítica, Nutrición y Bromatología, Campus Terra, Universidade da Santiago de Compostela, 27002 Lugo, SpainLaboratorio de Higiene, Inspección y Control de Alimentos, Departamento de Química Analítica, Nutrición y Bromatología, Campus Terra, Universidade da Santiago de Compostela, 27002 Lugo, SpainLaboratorio de Higiene, Inspección y Control de Alimentos, Departamento de Química Analítica, Nutrición y Bromatología, Campus Terra, Universidade da Santiago de Compostela, 27002 Lugo, SpainInstitute of Natural Sciences and Mathematics, Ural Federal University, Ekaterinburg 620075, RussiaInstitute of Natural Sciences and Mathematics, Ural Federal University, Ekaterinburg 620075, RussiaLaboratorio de Higiene, Inspección y Control de Alimentos, Departamento de Química Analítica, Nutrición y Bromatología, Campus Terra, Universidade da Santiago de Compostela, 27002 Lugo, SpainInstitute of Chemical Engineering, Ural Federal University, Ekaterinburg 620002, Russia<b>Purpose:</b> Immunodeficiency conditions, which are characterized by reduced immune activity that promotes the development of chronic diseases, are needed for efficient monitoring. A promising area of monitoring and early diagnosis of immunodeficiency diseases is the determination of metabolic biomarkers in the blood. <b>Methods:</b> In this work, we identified a set of lipid biomarkers of immunodeficiency states by performing high-performance liquid chromatography–high-resolution mass spectrometry (HPLC-HRMS) analysis of blood plasma samples from mice and processing them with bioinformatics approaches. Potential biomarkers were selected through statistical analysis and further validated by MS/MS. <b>Conclusions:</b> As a result, 15 lipids were confirmed and selected as potential biomarkers of immunodeficiency states. The selected biomarkers can be further studied and serve as promising targets for the early diagnosis of immunodeficiency diseases.https://www.mdpi.com/2218-1989/15/1/60lipidomicsmass spectrometrybiomarkersblood |
| spellingShingle | Ekaterina Demicheva Fernando Jonathan Polanco Espino Pavel Vedeneev Vadim Shevyrin Aleksey Buhler Elena Mukhlynina Olga Berdiugina Alicia del Carmen Mondragon Alberto Cepeda Sáez Aroa Lopez-Santamarina Alejandra Cardelle-Cobas Olga Solovyova Irina Danilova Jose Manuel Miranda Elena Kovaleva Comparative Study of Lipid Profile for Mice Treated with Cyclophosphamide by HPLC-HRMS and Bioinformatics Metabolites lipidomics mass spectrometry biomarkers blood |
| title | Comparative Study of Lipid Profile for Mice Treated with Cyclophosphamide by HPLC-HRMS and Bioinformatics |
| title_full | Comparative Study of Lipid Profile for Mice Treated with Cyclophosphamide by HPLC-HRMS and Bioinformatics |
| title_fullStr | Comparative Study of Lipid Profile for Mice Treated with Cyclophosphamide by HPLC-HRMS and Bioinformatics |
| title_full_unstemmed | Comparative Study of Lipid Profile for Mice Treated with Cyclophosphamide by HPLC-HRMS and Bioinformatics |
| title_short | Comparative Study of Lipid Profile for Mice Treated with Cyclophosphamide by HPLC-HRMS and Bioinformatics |
| title_sort | comparative study of lipid profile for mice treated with cyclophosphamide by hplc hrms and bioinformatics |
| topic | lipidomics mass spectrometry biomarkers blood |
| url | https://www.mdpi.com/2218-1989/15/1/60 |
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