The antibacterial activity of clove Syzygium aromaticum extract and its effects on the survival rate of hybrid grouper Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂ infected with Vibrio alginolyticus
Vibrio alginolyticus which becomes the main cause of vibriosis disease in grouper culture and causes great economic loss in Asian aquaculture industries. This study investigated the antibacterial activity of clove extract and the effect of adding clove powder to the diet on survival cantang grouper...
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Asosiasi Sains Akuakultur Indonesia
2023-01-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/43940 |
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| author | Inem Ode Sukenda Widanarni Dinamella Wahjuningrum Munti Yuhana Mia Setiawati |
| author_facet | Inem Ode Sukenda Widanarni Dinamella Wahjuningrum Munti Yuhana Mia Setiawati |
| author_sort | Inem Ode |
| collection | DOAJ |
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Vibrio alginolyticus which becomes the main cause of vibriosis disease in grouper culture and causes great economic loss in Asian aquaculture industries. This study investigated the antibacterial activity of clove extract and the effect of adding clove powder to the diet on survival cantang grouper infected with V. alginolyticus. The clove extraction used a maceration method. Two dose levels of clove powder were used, namely 10 and 15 g kg−1. The control treatments without clove powder supplementation contained positive control (CP) and negative control treatment (CN). The results found that the clove extract contained five major compounds. The highest compound was phenol, 2-methoxy-4-(2-propenyl)-Eugenol (64.07%). Phytochemical analysis of clove extract contained phenolics, flavonoids, and tannins at (28.53 ± 0.00) mg/g, (0.38 ± 0.00) mg/g, and (0.15 ± 0.00) %, respectively. The diameter of the clove extract inhibition zone was significantly different (P < 0.05) in all treatments. The Scanning Electron Microscopy (SEM) result presents that the clove extract can alter the V. alginolyticus cell morphology. The dietary supplementation of clove powder improves the survival rate significantly higher (P<0.05) post-challenge test. The conclusion of this research is that clove extract has antibacterial activity that can inhibit growth and cause cell morphological damage to V. alginolyticus. The application of clove powder at a dose of 15g kg-1 was able to improve the survival value which was a higher post-challenge test.
Keyword: antibacterial activity, clove, grouper, phytochemical, Vibrio alginolyticus
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| format | Article |
| id | doaj-art-d4a8def7e9e94685955c8ac3fecf8c21 |
| institution | Kabale University |
| issn | 1412-5269 2354-6700 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
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| series | Jurnal Akuakultur Indonesia |
| spelling | doaj-art-d4a8def7e9e94685955c8ac3fecf8c212025-01-24T17:56:09ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002023-01-0122110.19027/jai.22.1.1-1143940The antibacterial activity of clove Syzygium aromaticum extract and its effects on the survival rate of hybrid grouper Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂ infected with Vibrio alginolyticusInem Ode0Sukenda1Widanarni2Dinamella Wahjuningrum3Munti Yuhana4Mia Setiawati5Water Resource Management Study program, Faculty of Fisheries and Marine Sciences, Darussalam Ambon University, Ambon 97219, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java 16680, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java 16680, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java 16680, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java 16680, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java 16680, Indonesia Vibrio alginolyticus which becomes the main cause of vibriosis disease in grouper culture and causes great economic loss in Asian aquaculture industries. This study investigated the antibacterial activity of clove extract and the effect of adding clove powder to the diet on survival cantang grouper infected with V. alginolyticus. The clove extraction used a maceration method. Two dose levels of clove powder were used, namely 10 and 15 g kg−1. The control treatments without clove powder supplementation contained positive control (CP) and negative control treatment (CN). The results found that the clove extract contained five major compounds. The highest compound was phenol, 2-methoxy-4-(2-propenyl)-Eugenol (64.07%). Phytochemical analysis of clove extract contained phenolics, flavonoids, and tannins at (28.53 ± 0.00) mg/g, (0.38 ± 0.00) mg/g, and (0.15 ± 0.00) %, respectively. The diameter of the clove extract inhibition zone was significantly different (P < 0.05) in all treatments. The Scanning Electron Microscopy (SEM) result presents that the clove extract can alter the V. alginolyticus cell morphology. The dietary supplementation of clove powder improves the survival rate significantly higher (P<0.05) post-challenge test. The conclusion of this research is that clove extract has antibacterial activity that can inhibit growth and cause cell morphological damage to V. alginolyticus. The application of clove powder at a dose of 15g kg-1 was able to improve the survival value which was a higher post-challenge test. Keyword: antibacterial activity, clove, grouper, phytochemical, Vibrio alginolyticus https://journal.ipb.ac.id/index.php/jai/article/view/43940 |
| spellingShingle | Inem Ode Sukenda Widanarni Dinamella Wahjuningrum Munti Yuhana Mia Setiawati The antibacterial activity of clove Syzygium aromaticum extract and its effects on the survival rate of hybrid grouper Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂ infected with Vibrio alginolyticus Jurnal Akuakultur Indonesia |
| title | The antibacterial activity of clove Syzygium aromaticum extract and its effects on the survival rate of hybrid grouper Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂ infected with Vibrio alginolyticus |
| title_full | The antibacterial activity of clove Syzygium aromaticum extract and its effects on the survival rate of hybrid grouper Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂ infected with Vibrio alginolyticus |
| title_fullStr | The antibacterial activity of clove Syzygium aromaticum extract and its effects on the survival rate of hybrid grouper Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂ infected with Vibrio alginolyticus |
| title_full_unstemmed | The antibacterial activity of clove Syzygium aromaticum extract and its effects on the survival rate of hybrid grouper Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂ infected with Vibrio alginolyticus |
| title_short | The antibacterial activity of clove Syzygium aromaticum extract and its effects on the survival rate of hybrid grouper Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂ infected with Vibrio alginolyticus |
| title_sort | antibacterial activity of clove syzygium aromaticum extract and its effects on the survival rate of hybrid grouper epinephelus fuscoguttatus ♀ e lanceolatus ♂ infected with vibrio alginolyticus |
| url | https://journal.ipb.ac.id/index.php/jai/article/view/43940 |
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