Spatial Distribution of PCB Dechlorinating Bacteria and Activities in Contaminated Soil
Soil samples contaminated with Aroclor 1260 were analyzed for microbial PCB dechlorination potential, which is the rate-limiting step for complete PCB degradation. The average chlorines per biphenyl varied throughout the site suggesting that different rates of in situ dechlorination had occurred ov...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Applied and Environmental Soil Science |
| Online Access: | http://dx.doi.org/10.1155/2012/584970 |
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| author | Birthe V. Kjellerup Piuly Paul Upal Ghosh Harold D. May Kevin R. Sowers |
| author_facet | Birthe V. Kjellerup Piuly Paul Upal Ghosh Harold D. May Kevin R. Sowers |
| author_sort | Birthe V. Kjellerup |
| collection | DOAJ |
| description | Soil samples contaminated with Aroclor 1260 were analyzed for microbial PCB dechlorination potential, which is the rate-limiting step for complete PCB degradation. The average chlorines per biphenyl varied throughout the site suggesting that different rates of in situ dechlorination had occurred over time. Analysis of PCB transforming (aerobic and anaerobic) microbial communities and dechlorinating potential revealed spatial heterogeneity of both putative PCB transforming phylotypes and dechlorination activity. Some soil samples inhibited PCB dechlorination in active sediment from Baltimore Harbor indicating that metal or organic cocontaminants might cause the observed heterogeneity of in situ dechlorination. Bioaugmentation of soil samples contaminated with PCBs ranging from 4.6 to 265 ppm with a pure culture of the PCB dechlorinating bacterium Dehalobium chlorocoercia DF-1 also yielded heterologous results with significant dechlorination of weathered PCBs observed in one location. The detection of indigenous PCB dehalorespiring activity combined with the detection of putative dechlorinating bacteria and biphenyl dioxygenase genes in the soil aggregates suggests that the potential exists for complete mineralization of PCBs in soils. However, in contrast to sediments, the heterologous distribution of microorganisms, PCBs, and inhibitory cocontaminants is a significant challenge for the development of in situ microbial treatment of PCB impacted soils. |
| format | Article |
| id | doaj-art-d3bee0bf1f7e458490eecb08eb01c995 |
| institution | Kabale University |
| issn | 1687-7667 1687-7675 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applied and Environmental Soil Science |
| spelling | doaj-art-d3bee0bf1f7e458490eecb08eb01c9952025-02-03T01:30:28ZengWileyApplied and Environmental Soil Science1687-76671687-76752012-01-01201210.1155/2012/584970584970Spatial Distribution of PCB Dechlorinating Bacteria and Activities in Contaminated SoilBirthe V. Kjellerup0Piuly Paul1Upal Ghosh2Harold D. May3Kevin R. Sowers4Department of Biological Sciences, Goucher College, 1021 Dulaney Valley Road, Baltimore, MD 21204, USADepartment of Civil and Environmental Engineering, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USADepartment of Civil and Environmental Engineering, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USADepartment of Microbiology & Immunology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425, USADepartment of Marine Biotechnology, University of Maryland Baltimore County, 701 E. Pratt Street, Baltimore, MD 21202, USASoil samples contaminated with Aroclor 1260 were analyzed for microbial PCB dechlorination potential, which is the rate-limiting step for complete PCB degradation. The average chlorines per biphenyl varied throughout the site suggesting that different rates of in situ dechlorination had occurred over time. Analysis of PCB transforming (aerobic and anaerobic) microbial communities and dechlorinating potential revealed spatial heterogeneity of both putative PCB transforming phylotypes and dechlorination activity. Some soil samples inhibited PCB dechlorination in active sediment from Baltimore Harbor indicating that metal or organic cocontaminants might cause the observed heterogeneity of in situ dechlorination. Bioaugmentation of soil samples contaminated with PCBs ranging from 4.6 to 265 ppm with a pure culture of the PCB dechlorinating bacterium Dehalobium chlorocoercia DF-1 also yielded heterologous results with significant dechlorination of weathered PCBs observed in one location. The detection of indigenous PCB dehalorespiring activity combined with the detection of putative dechlorinating bacteria and biphenyl dioxygenase genes in the soil aggregates suggests that the potential exists for complete mineralization of PCBs in soils. However, in contrast to sediments, the heterologous distribution of microorganisms, PCBs, and inhibitory cocontaminants is a significant challenge for the development of in situ microbial treatment of PCB impacted soils.http://dx.doi.org/10.1155/2012/584970 |
| spellingShingle | Birthe V. Kjellerup Piuly Paul Upal Ghosh Harold D. May Kevin R. Sowers Spatial Distribution of PCB Dechlorinating Bacteria and Activities in Contaminated Soil Applied and Environmental Soil Science |
| title | Spatial Distribution of PCB Dechlorinating Bacteria and Activities in Contaminated Soil |
| title_full | Spatial Distribution of PCB Dechlorinating Bacteria and Activities in Contaminated Soil |
| title_fullStr | Spatial Distribution of PCB Dechlorinating Bacteria and Activities in Contaminated Soil |
| title_full_unstemmed | Spatial Distribution of PCB Dechlorinating Bacteria and Activities in Contaminated Soil |
| title_short | Spatial Distribution of PCB Dechlorinating Bacteria and Activities in Contaminated Soil |
| title_sort | spatial distribution of pcb dechlorinating bacteria and activities in contaminated soil |
| url | http://dx.doi.org/10.1155/2012/584970 |
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