Adenosine cyclic phosphate with ultrasonic-assisted pectinase extraction alleviated allergic reactions in RBL-2H3 through inhibiting the influx of intracellular Ca2+
Jujube contains abundant cyclic adenosine monophosphate (cAMP) and the ultrasonic-assisted pectinase extraction (UAPE) conditions for obtaining the maximum cAMP yield from jujube were optimized. Orthogonal array design was applied to evaluate the effects of 4 variables by UAPE on cAMP yield. The res...
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Tsinghua University Press
2023-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002105 |
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| author | Qiao Bai Xiaoping Feng Yu Wang Chunyu Wu Ye Liu Jiao Sun Tianli Yue Fangyu Long |
| author_facet | Qiao Bai Xiaoping Feng Yu Wang Chunyu Wu Ye Liu Jiao Sun Tianli Yue Fangyu Long |
| author_sort | Qiao Bai |
| collection | DOAJ |
| description | Jujube contains abundant cyclic adenosine monophosphate (cAMP) and the ultrasonic-assisted pectinase extraction (UAPE) conditions for obtaining the maximum cAMP yield from jujube were optimized. Orthogonal array design was applied to evaluate the effects of 4 variables by UAPE on cAMP yield. The results showed that the optimal cAMP yield (783.0 μg/g) was derived at ratio of liquid to solid 5 mL/g, ratio of pectinase to raw material 1.5 %, time 60 min and temperature 40 °C. Moreover, the effect of cAMP on the anti-allergic function of action induced by immunoglobulin E (IgE) and its meschanism was investigated through establishing the sensitized cell model in rat basophilic leukemia (RBL-2H3) cells using dinitrophenylated (DNP)-bovine serum albumin (BSA)-IgE. The results showed that cAMP interfered with sensitized cells, effectively inhibited the occurrence of basophil degranulation in dose dependence, and significantly reduced the activity of β-hexosamindase (β-hex), at the optimal concentration of 50 μg/mL. The level of anti-inflammatory factor interleukin-10 (IL-10) was promoted and the content of pro-inflammatory factor tumor necrosis factor-α (TNF-α) was suppressed by cAMP. In addition, influx of intracellular Ca2+ was repressed effectively. Our results demonstrate that jujube cAMP regulated the cytokine balance in the allergy pathway through blocking the influx of extracellular Ca2+, with the prevention of allergy symptoms. |
| format | Article |
| id | doaj-art-d35240f59adb45fb93dfe5c4cf562919 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-d35240f59adb45fb93dfe5c4cf5629192025-02-03T05:15:35ZengTsinghua University PressFood Science and Human Wellness2213-45302023-05-01123832841Adenosine cyclic phosphate with ultrasonic-assisted pectinase extraction alleviated allergic reactions in RBL-2H3 through inhibiting the influx of intracellular Ca2+Qiao Bai0Xiaoping Feng1Yu Wang2Chunyu Wu3Ye Liu4Jiao Sun5Tianli Yue6Fangyu Long7College of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaBeijing Key Laboratory of Plant Resource Research and Development, Beijing Technology and Business University, Beijing 100048, ChinaDepartment of Breast Surgery, the Affiliated Hospital of Qingdao University, Qingdao 266000, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, China; Corresponding authors at: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, China; Corresponding authors at: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaJujube contains abundant cyclic adenosine monophosphate (cAMP) and the ultrasonic-assisted pectinase extraction (UAPE) conditions for obtaining the maximum cAMP yield from jujube were optimized. Orthogonal array design was applied to evaluate the effects of 4 variables by UAPE on cAMP yield. The results showed that the optimal cAMP yield (783.0 μg/g) was derived at ratio of liquid to solid 5 mL/g, ratio of pectinase to raw material 1.5 %, time 60 min and temperature 40 °C. Moreover, the effect of cAMP on the anti-allergic function of action induced by immunoglobulin E (IgE) and its meschanism was investigated through establishing the sensitized cell model in rat basophilic leukemia (RBL-2H3) cells using dinitrophenylated (DNP)-bovine serum albumin (BSA)-IgE. The results showed that cAMP interfered with sensitized cells, effectively inhibited the occurrence of basophil degranulation in dose dependence, and significantly reduced the activity of β-hexosamindase (β-hex), at the optimal concentration of 50 μg/mL. The level of anti-inflammatory factor interleukin-10 (IL-10) was promoted and the content of pro-inflammatory factor tumor necrosis factor-α (TNF-α) was suppressed by cAMP. In addition, influx of intracellular Ca2+ was repressed effectively. Our results demonstrate that jujube cAMP regulated the cytokine balance in the allergy pathway through blocking the influx of extracellular Ca2+, with the prevention of allergy symptoms.http://www.sciencedirect.com/science/article/pii/S2213453022002105ultrasonic-assisted pectinase extractioncyclic adenosine monophosphaterat basophilic leukemia cellsCa2+ influxAllergy |
| spellingShingle | Qiao Bai Xiaoping Feng Yu Wang Chunyu Wu Ye Liu Jiao Sun Tianli Yue Fangyu Long Adenosine cyclic phosphate with ultrasonic-assisted pectinase extraction alleviated allergic reactions in RBL-2H3 through inhibiting the influx of intracellular Ca2+ Food Science and Human Wellness ultrasonic-assisted pectinase extraction cyclic adenosine monophosphate rat basophilic leukemia cells Ca2+ influx Allergy |
| title | Adenosine cyclic phosphate with ultrasonic-assisted pectinase extraction alleviated allergic reactions in RBL-2H3 through inhibiting the influx of intracellular Ca2+ |
| title_full | Adenosine cyclic phosphate with ultrasonic-assisted pectinase extraction alleviated allergic reactions in RBL-2H3 through inhibiting the influx of intracellular Ca2+ |
| title_fullStr | Adenosine cyclic phosphate with ultrasonic-assisted pectinase extraction alleviated allergic reactions in RBL-2H3 through inhibiting the influx of intracellular Ca2+ |
| title_full_unstemmed | Adenosine cyclic phosphate with ultrasonic-assisted pectinase extraction alleviated allergic reactions in RBL-2H3 through inhibiting the influx of intracellular Ca2+ |
| title_short | Adenosine cyclic phosphate with ultrasonic-assisted pectinase extraction alleviated allergic reactions in RBL-2H3 through inhibiting the influx of intracellular Ca2+ |
| title_sort | adenosine cyclic phosphate with ultrasonic assisted pectinase extraction alleviated allergic reactions in rbl 2h3 through inhibiting the influx of intracellular ca2 |
| topic | ultrasonic-assisted pectinase extraction cyclic adenosine monophosphate rat basophilic leukemia cells Ca2+ influx Allergy |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002105 |
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