Xanthomonas citri subsp. citri requires a genus-specific outer membrane protein and TolB to coordinate cell membrane integrity and virulence
ABSTRACT Xanthomonas citri subsp. citri (Xcc) possesses a Xanthomonas-specific outer membrane protein XAC1347 (OMPXan) that exerts a role in the expression of the type III secretion system for pathogenicity. In this study, we reported that OMPXan was required for salt stress tolerance and cell membr...
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American Society for Microbiology
2025-02-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.02521-24 |
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| author | Leilei Wu Xueyan Zhu Hang Long Xinyu Huang Shuying Zhu Qi Xue Ziao Li Huasong Zou |
| author_facet | Leilei Wu Xueyan Zhu Hang Long Xinyu Huang Shuying Zhu Qi Xue Ziao Li Huasong Zou |
| author_sort | Leilei Wu |
| collection | DOAJ |
| description | ABSTRACT Xanthomonas citri subsp. citri (Xcc) possesses a Xanthomonas-specific outer membrane protein XAC1347 (OMPXan) that exerts a role in the expression of the type III secretion system for pathogenicity. In this study, we reported that OMPXan was required for salt stress tolerance and cell membrane integrity, as well as the expression of the gum genes for the production of extracellular polysaccharides. Pull-down and yeast two-hybrid assays revealed that OMPXan interacts with TolB, a substrate of the Tol/Pal membrane protein system. The deletion of tolB resulted in similar phenotypic alterations as the OMPXan mutant in salt stress tolerance, cell membrane integrity, and the expression of hrpG, hrpX, hrpD6, and hrcC for pathogenicity. In contrast, the absence of TolB resulted in an increased level of expression of the gum genes and the production of extracellular polysaccharides. These results indicate that the interaction of OMPXan and TolB coordinates multi-faceted mechanisms to manage environmental stress and pathogenicity.IMPORTANCEThe gram-negative Xanthomoas citri subsp. citri (Xcc) is a causal agent of citrus canker, a serious bacterial disease on citrus plants. Our previous research reported that a Xanthomonas-specific outer membrane protein XAC1347 (OMPXan) is necessary for type III gene expression. This manuscript provided evidence to show that OMPXan interacts with Tol/Pal system substrate TolB. Moreover, OMPXan and TolB are both required for cell membrane integrity, stress adaption, and virulence. The overall results support that OMPXan and TolB coordinate multi-faceted mechanisms to manage environmental stress and pathogenicity. |
| format | Article |
| id | doaj-art-d2c5a0c0c91c487bae9eb99aedf744ed |
| institution | Kabale University |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj-art-d2c5a0c0c91c487bae9eb99aedf744ed2025-02-04T14:03:41ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-02-0113210.1128/spectrum.02521-24Xanthomonas citri subsp. citri requires a genus-specific outer membrane protein and TolB to coordinate cell membrane integrity and virulenceLeilei Wu0Xueyan Zhu1Hang Long2Xinyu Huang3Shuying Zhu4Qi Xue5Ziao Li6Huasong Zou7School of Life Sciences and Health, Huzhou College, Huzhou, Zhejiang, ChinaSchool of Life Sciences and Health, Huzhou College, Huzhou, Zhejiang, ChinaSchool of Life Sciences and Health, Huzhou College, Huzhou, Zhejiang, ChinaSchool of Life Sciences and Health, Huzhou College, Huzhou, Zhejiang, ChinaSchool of Life Sciences and Health, Huzhou College, Huzhou, Zhejiang, ChinaSchool of Life Sciences and Health, Huzhou College, Huzhou, Zhejiang, ChinaSchool of Life Sciences and Health, Huzhou College, Huzhou, Zhejiang, ChinaSchool of Life Sciences and Health, Huzhou College, Huzhou, Zhejiang, ChinaABSTRACT Xanthomonas citri subsp. citri (Xcc) possesses a Xanthomonas-specific outer membrane protein XAC1347 (OMPXan) that exerts a role in the expression of the type III secretion system for pathogenicity. In this study, we reported that OMPXan was required for salt stress tolerance and cell membrane integrity, as well as the expression of the gum genes for the production of extracellular polysaccharides. Pull-down and yeast two-hybrid assays revealed that OMPXan interacts with TolB, a substrate of the Tol/Pal membrane protein system. The deletion of tolB resulted in similar phenotypic alterations as the OMPXan mutant in salt stress tolerance, cell membrane integrity, and the expression of hrpG, hrpX, hrpD6, and hrcC for pathogenicity. In contrast, the absence of TolB resulted in an increased level of expression of the gum genes and the production of extracellular polysaccharides. These results indicate that the interaction of OMPXan and TolB coordinates multi-faceted mechanisms to manage environmental stress and pathogenicity.IMPORTANCEThe gram-negative Xanthomoas citri subsp. citri (Xcc) is a causal agent of citrus canker, a serious bacterial disease on citrus plants. Our previous research reported that a Xanthomonas-specific outer membrane protein XAC1347 (OMPXan) is necessary for type III gene expression. This manuscript provided evidence to show that OMPXan interacts with Tol/Pal system substrate TolB. Moreover, OMPXan and TolB are both required for cell membrane integrity, stress adaption, and virulence. The overall results support that OMPXan and TolB coordinate multi-faceted mechanisms to manage environmental stress and pathogenicity.https://journals.asm.org/doi/10.1128/spectrum.02521-24citrus cankerXanthomonasTolBouter membrane proteinTol/Palmembrane integrity |
| spellingShingle | Leilei Wu Xueyan Zhu Hang Long Xinyu Huang Shuying Zhu Qi Xue Ziao Li Huasong Zou Xanthomonas citri subsp. citri requires a genus-specific outer membrane protein and TolB to coordinate cell membrane integrity and virulence Microbiology Spectrum citrus canker Xanthomonas TolB outer membrane protein Tol/Pal membrane integrity |
| title | Xanthomonas citri subsp. citri requires a genus-specific outer membrane protein and TolB to coordinate cell membrane integrity and virulence |
| title_full | Xanthomonas citri subsp. citri requires a genus-specific outer membrane protein and TolB to coordinate cell membrane integrity and virulence |
| title_fullStr | Xanthomonas citri subsp. citri requires a genus-specific outer membrane protein and TolB to coordinate cell membrane integrity and virulence |
| title_full_unstemmed | Xanthomonas citri subsp. citri requires a genus-specific outer membrane protein and TolB to coordinate cell membrane integrity and virulence |
| title_short | Xanthomonas citri subsp. citri requires a genus-specific outer membrane protein and TolB to coordinate cell membrane integrity and virulence |
| title_sort | xanthomonas citri subsp citri requires a genus specific outer membrane protein and tolb to coordinate cell membrane integrity and virulence |
| topic | citrus canker Xanthomonas TolB outer membrane protein Tol/Pal membrane integrity |
| url | https://journals.asm.org/doi/10.1128/spectrum.02521-24 |
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