Protocol for culture, purification, and target validation of a hybridoma-generated monoclonal antibody targeting Aβ truncated species

Protocol for culture, purification, and target validation of a hybridoma-generated monoclonal antibody targeting Aβ truncated species

Summary: Alzheimer’s disease (AD) is characterized by the deposition of full-length and truncated amyloid beta (Aβ) species within brain parenchyma and cerebral vessels. However, Aβ truncated species remain understudied. Here, we present a protocol for culture and characterization of a mouse monoclo...

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Bibliographic Details
Main Authors: Maria Luisa Valle, Bitseat Getaneh, James Loveland, Hediye Erdjument-Bromage, Christopher William, Thomas A. Neubert, Agueda Rostagno, Jorge Ghiso
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
Molecular Biology
Antibody
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725002709
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http://www.sciencedirect.com/science/article/pii/S2666166725002709

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