microRNA-21对膀胱癌T24细胞增殖的影响 及对VEGF和AP1蛋白的调节
【目的】 研究microRNA-21(miR-21)对膀胱癌T24细胞系中血管内皮生长因子(vascular endothelial growth factor, VEGF)和活化因子蛋白-1(activator protein-1, AP1)及mRNA表达的调节作用,探讨miR-21对膀胱癌T24细胞增殖的影响65377; 【方法】 用人工合成的miR-21相应的双链互补DNA片段,插入pSilencerTM 3.1-H1 neo载体表达载体,经测序鉴定后作为microRNA的表达质粒;用脂质体将miR-21高表达质粒转染进T24膀胱癌细胞,经G418筛选获得阳性克隆65377;用RT-PC...
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| Format: | Article |
|---|---|
| Language: | zho |
| Published: |
Editorial Office of Journal of Sun Yat-sen University
2010-01-01
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| Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
| Subjects: | |
| Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/43578007/ |
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