Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvatica
We compared physiological characteristics and responses to experimental freezing and thawing in winter and spring samples of the wood frog, Rana sylvatica, indigenous to Interior Alaska, USA. Whereas winter frogs can survive freezing at temperatures at least as low as −16°C, the lower limit of toler...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Zoology |
| Online Access: | http://dx.doi.org/10.1155/2014/750153 |
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| author | Jon P. Costanzo M. Clara F. do Amaral Andrew J. Rosendale Richard E. Lee |
| author_facet | Jon P. Costanzo M. Clara F. do Amaral Andrew J. Rosendale Richard E. Lee |
| author_sort | Jon P. Costanzo |
| collection | DOAJ |
| description | We compared physiological characteristics and responses to experimental freezing and thawing in winter and spring samples of the wood frog, Rana sylvatica, indigenous to Interior Alaska, USA. Whereas winter frogs can survive freezing at temperatures at least as low as −16°C, the lower limit of tolerance for spring frogs was between −2.5°C and −5°C. Spring frogs had comparatively low levels of the urea in blood plasma, liver, heart, brain, and skeletal muscle, as well as a smaller hepatic reserve of glycogen, which is converted to glucose after freezing begins. Consequently, following freezing (−2.5°C, 48 h) tissue concentrations of these cryoprotective osmolytes were 44–88% lower than those measured in winter frogs. Spring frogs formed much more ice and incurred extensive cryohemolysis and lactate accrual, indicating that they had suffered marked cell damage and hypoxic stress during freezing. Multiple, interactive stresses, in addition to diminished cryoprotectant levels, contribute to the reduced capacity for freeze tolerance in posthibernal frogs. |
| format | Article |
| id | doaj-art-d146ee5d45c6410d8f63fc6937d11dd1 |
| institution | Kabale University |
| issn | 1687-8477 1687-8485 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Zoology |
| spelling | doaj-art-d146ee5d45c6410d8f63fc6937d11dd12025-02-03T01:02:00ZengWileyInternational Journal of Zoology1687-84771687-84852014-01-01201410.1155/2014/750153750153Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvaticaJon P. Costanzo0M. Clara F. do Amaral1Andrew J. Rosendale2Richard E. Lee3Department of Zoology, Miami University, Oxford, OH 45056, USADepartment of Zoology, Miami University, Oxford, OH 45056, USADepartment of Zoology, Miami University, Oxford, OH 45056, USADepartment of Zoology, Miami University, Oxford, OH 45056, USAWe compared physiological characteristics and responses to experimental freezing and thawing in winter and spring samples of the wood frog, Rana sylvatica, indigenous to Interior Alaska, USA. Whereas winter frogs can survive freezing at temperatures at least as low as −16°C, the lower limit of tolerance for spring frogs was between −2.5°C and −5°C. Spring frogs had comparatively low levels of the urea in blood plasma, liver, heart, brain, and skeletal muscle, as well as a smaller hepatic reserve of glycogen, which is converted to glucose after freezing begins. Consequently, following freezing (−2.5°C, 48 h) tissue concentrations of these cryoprotective osmolytes were 44–88% lower than those measured in winter frogs. Spring frogs formed much more ice and incurred extensive cryohemolysis and lactate accrual, indicating that they had suffered marked cell damage and hypoxic stress during freezing. Multiple, interactive stresses, in addition to diminished cryoprotectant levels, contribute to the reduced capacity for freeze tolerance in posthibernal frogs.http://dx.doi.org/10.1155/2014/750153 |
| spellingShingle | Jon P. Costanzo M. Clara F. do Amaral Andrew J. Rosendale Richard E. Lee Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvatica International Journal of Zoology |
| title | Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvatica |
| title_full | Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvatica |
| title_fullStr | Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvatica |
| title_full_unstemmed | Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvatica |
| title_short | Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvatica |
| title_sort | seasonality of freeze tolerance in a subarctic population of the wood frog rana sylvatica |
| url | http://dx.doi.org/10.1155/2014/750153 |
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