Comparison of Two Methods for Assaying Reducing Sugars in the Determination of Carbohydrase Activities
The Nelson-Somogyi (NS) and 3,5-dinitrosalicylic acid (DNS) assays for reducing sugars are widely used in measurements of carbohydrase activities against different polysaccharides. Using twelve commercial enzyme preparations, the comparison of the NS and DNS assays in determination of cellulase, β-g...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2011/283658 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832550519527178240 |
|---|---|
| author | Alexander V. Gusakov Elena G. Kondratyeva Arkady P. Sinitsyn |
| author_facet | Alexander V. Gusakov Elena G. Kondratyeva Arkady P. Sinitsyn |
| author_sort | Alexander V. Gusakov |
| collection | DOAJ |
| description | The Nelson-Somogyi (NS) and 3,5-dinitrosalicylic acid (DNS) assays for
reducing sugars are widely used in measurements of carbohydrase activities against different
polysaccharides. Using twelve commercial enzyme preparations, the comparison of the NS and DNS
assays in determination of cellulase, β-glucanase, xylanase, and β-mannanase activities was carried out. When cellulase activities against CMC were measured,
the DNS assay gave activity values, which were typically 40–50% higher than those obtained with the NS assay.
In the analysis of the xylanase, β-mannanase, and β-glucanase activities, the overestimations by the DNS assay were much more pronounced (the observed differences in the activities were 3- to 13-fold). Reasons for preferential use of
the NS assay for measuring activities of carbohydrases other than cellulases are discussed. |
| format | Article |
| id | doaj-art-d1295e3e84824ce9a25c5af69b9c6e6d |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-d1295e3e84824ce9a25c5af69b9c6e6d2025-02-03T06:06:36ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792011-01-01201110.1155/2011/283658283658Comparison of Two Methods for Assaying Reducing Sugars in the Determination of Carbohydrase ActivitiesAlexander V. Gusakov0Elena G. Kondratyeva1Arkady P. Sinitsyn2Department of Chemistry, M. V. Lomonosov Moscow State University, Moscow 119991, RussiaA. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow 119071, RussiaDepartment of Chemistry, M. V. Lomonosov Moscow State University, Moscow 119991, RussiaThe Nelson-Somogyi (NS) and 3,5-dinitrosalicylic acid (DNS) assays for reducing sugars are widely used in measurements of carbohydrase activities against different polysaccharides. Using twelve commercial enzyme preparations, the comparison of the NS and DNS assays in determination of cellulase, β-glucanase, xylanase, and β-mannanase activities was carried out. When cellulase activities against CMC were measured, the DNS assay gave activity values, which were typically 40–50% higher than those obtained with the NS assay. In the analysis of the xylanase, β-mannanase, and β-glucanase activities, the overestimations by the DNS assay were much more pronounced (the observed differences in the activities were 3- to 13-fold). Reasons for preferential use of the NS assay for measuring activities of carbohydrases other than cellulases are discussed.http://dx.doi.org/10.1155/2011/283658 |
| spellingShingle | Alexander V. Gusakov Elena G. Kondratyeva Arkady P. Sinitsyn Comparison of Two Methods for Assaying Reducing Sugars in the Determination of Carbohydrase Activities International Journal of Analytical Chemistry |
| title | Comparison of Two Methods for Assaying Reducing Sugars in the Determination of Carbohydrase Activities |
| title_full | Comparison of Two Methods for Assaying Reducing Sugars in the Determination of Carbohydrase Activities |
| title_fullStr | Comparison of Two Methods for Assaying Reducing Sugars in the Determination of Carbohydrase Activities |
| title_full_unstemmed | Comparison of Two Methods for Assaying Reducing Sugars in the Determination of Carbohydrase Activities |
| title_short | Comparison of Two Methods for Assaying Reducing Sugars in the Determination of Carbohydrase Activities |
| title_sort | comparison of two methods for assaying reducing sugars in the determination of carbohydrase activities |
| url | http://dx.doi.org/10.1155/2011/283658 |
| work_keys_str_mv | AT alexandervgusakov comparisonoftwomethodsforassayingreducingsugarsinthedeterminationofcarbohydraseactivities AT elenagkondratyeva comparisonoftwomethodsforassayingreducingsugarsinthedeterminationofcarbohydraseactivities AT arkadypsinitsyn comparisonoftwomethodsforassayingreducingsugarsinthedeterminationofcarbohydraseactivities |