Transcriptomic and physiological analyses reveal that cytokinin is involved in the compound leaf development of alfalfa
Alfalfa is one of the primary forages, and its yield is largely dependent on the development of its leaf. In this study, to elucidate the mechanism of compound leaf development, we isolated and examined the alfalfa phenotype Chuancao No.7, exhibiting five leaflets. The agronomic traits of the Chuanc...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1460205/full |
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| author | Hongyao Mei Jiajun Yan Xuexin Jia Weilin Wang Shuangshuang Li Ruiqi Sun Hongjiao Jiang Lijun Xie Chuanen Zhou Shiqie Bai Lu Han |
| author_facet | Hongyao Mei Jiajun Yan Xuexin Jia Weilin Wang Shuangshuang Li Ruiqi Sun Hongjiao Jiang Lijun Xie Chuanen Zhou Shiqie Bai Lu Han |
| author_sort | Hongyao Mei |
| collection | DOAJ |
| description | Alfalfa is one of the primary forages, and its yield is largely dependent on the development of its leaf. In this study, to elucidate the mechanism of compound leaf development, we isolated and examined the alfalfa phenotype Chuancao No.7, exhibiting five leaflets. The agronomic traits of the Chuancao No.7 including the leaf blade area, leaf/stem ratio, total fresh weight, and dry weight showed significant increases compared to those of the wild-type. Analyses of forage quality traits indicated significant differences in crude protein (CP), acid detergent fiber (ADF), crude fat (CF), water-soluble sugars (WSS), carbon content, ash composition content, and phosphorus content between Chuancao No.7 alfalfa and wild-type. Transcriptomic profile analysis revealed that differentially expressed genes were identified in the cytokinin (CK) signaling pathway. Both exogenous treatment and endogenous CK content detection indicated that cytokinin played a key role in the development of the alfalfa compound leaf. These results serve as a valuable resource for optimizing the forage quality and exploring the excellent germplasm of alfalfa. |
| format | Article |
| id | doaj-art-d01ad844add34e77871909c28ad5f053 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-d01ad844add34e77871909c28ad5f0532025-01-29T06:46:06ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011610.3389/fpls.2025.14602051460205Transcriptomic and physiological analyses reveal that cytokinin is involved in the compound leaf development of alfalfaHongyao Mei0Jiajun Yan1Xuexin Jia2Weilin Wang3Shuangshuang Li4Ruiqi Sun5Hongjiao Jiang6Lijun Xie7Chuanen Zhou8Shiqie Bai9Lu Han10The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, ChinaAlfalfa is one of the primary forages, and its yield is largely dependent on the development of its leaf. In this study, to elucidate the mechanism of compound leaf development, we isolated and examined the alfalfa phenotype Chuancao No.7, exhibiting five leaflets. The agronomic traits of the Chuancao No.7 including the leaf blade area, leaf/stem ratio, total fresh weight, and dry weight showed significant increases compared to those of the wild-type. Analyses of forage quality traits indicated significant differences in crude protein (CP), acid detergent fiber (ADF), crude fat (CF), water-soluble sugars (WSS), carbon content, ash composition content, and phosphorus content between Chuancao No.7 alfalfa and wild-type. Transcriptomic profile analysis revealed that differentially expressed genes were identified in the cytokinin (CK) signaling pathway. Both exogenous treatment and endogenous CK content detection indicated that cytokinin played a key role in the development of the alfalfa compound leaf. These results serve as a valuable resource for optimizing the forage quality and exploring the excellent germplasm of alfalfa.https://www.frontiersin.org/articles/10.3389/fpls.2025.1460205/fullalfalfacompound leaf developmentcytokininforage qualityKNOX1 |
| spellingShingle | Hongyao Mei Jiajun Yan Xuexin Jia Weilin Wang Shuangshuang Li Ruiqi Sun Hongjiao Jiang Lijun Xie Chuanen Zhou Shiqie Bai Lu Han Transcriptomic and physiological analyses reveal that cytokinin is involved in the compound leaf development of alfalfa Frontiers in Plant Science alfalfa compound leaf development cytokinin forage quality KNOX1 |
| title | Transcriptomic and physiological analyses reveal that cytokinin is involved in the compound leaf development of alfalfa |
| title_full | Transcriptomic and physiological analyses reveal that cytokinin is involved in the compound leaf development of alfalfa |
| title_fullStr | Transcriptomic and physiological analyses reveal that cytokinin is involved in the compound leaf development of alfalfa |
| title_full_unstemmed | Transcriptomic and physiological analyses reveal that cytokinin is involved in the compound leaf development of alfalfa |
| title_short | Transcriptomic and physiological analyses reveal that cytokinin is involved in the compound leaf development of alfalfa |
| title_sort | transcriptomic and physiological analyses reveal that cytokinin is involved in the compound leaf development of alfalfa |
| topic | alfalfa compound leaf development cytokinin forage quality KNOX1 |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1460205/full |
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