Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing
Embryonic miscarriage severely affects the life quality and physical and mental state of pregnant women. However, the detailed mechanism underlying embryonic miscarriage is not fully understood. This study is aimed at analyzing embryonic miscarriage. We collected samples from 25 normal pregnant wome...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2020/1764959 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832550936748228608 |
|---|---|
| author | Linfen Xu Lingna Huang Chengying Lian Huili Xue Yanfang Lu Xiujuan Chen Yong Xia |
| author_facet | Linfen Xu Lingna Huang Chengying Lian Huili Xue Yanfang Lu Xiujuan Chen Yong Xia |
| author_sort | Linfen Xu |
| collection | DOAJ |
| description | Embryonic miscarriage severely affects the life quality and physical and mental state of pregnant women. However, the detailed mechanism underlying embryonic miscarriage is not fully understood. This study is aimed at analyzing embryonic miscarriage. We collected samples from 25 normal pregnant women and 25 embryonic miscarriage patients of similar age to analyze microbiota isolated from the vagina. Crude examination of the vagina isolates showed that compared with the control group, 80% of the embryonic miscarriage group contained a significantly lower number of Lactobacillus, the major healthy microbe in the vagina. Furthermore, the levels of Th1 and Th2 secreted cytokine interleukin 2 (IL-2) and interleukin10 (IL-10), respectively, were examined. Results showed that the IL2 level was higher, and IL10 level was lower in the embryonic miscarriage group than in the control group, whereas the IL2/IL10 level was higher in the embryonic miscarriage group than in the control group. This finding suggested that the immune response was suppressed in the embryonic miscarriage group. To further dissect the microbiota of the vagina in the two groups, 16S rDNA sequencing was performed. Bioinformatics analysis showed that 1096 and 998 overlapped operational taxonomic units were identified from the embryonic miscarriage and control groups, respectively. At the genus level, the relative abundance of Fam_Finegoldia, Lac_Coprococcus_3, and Lac_Roseburia significantly differed in the embryonic miscarriage group. Overall, our analyses provided potential biomarkers for embryonic miscarriage and elucidated the causative relationship between microbiota and immune responses and may enable the possible diagnosis and therapeutics of early pregnancy loss. |
| format | Article |
| id | doaj-art-d0117e4ef5e14b5fa2e71a926c460776 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-d0117e4ef5e14b5fa2e71a926c4607762025-02-03T06:05:29ZengWileyInternational Journal of Genomics2314-436X2314-43782020-01-01202010.1155/2020/17649591764959Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput SequencingLinfen Xu0Lingna Huang1Chengying Lian2Huili Xue3Yanfang Lu4Xiujuan Chen5Yong Xia6Department of Gynaecology, Fujian Provincial Maternity and Children’s Hospital, Fujian Medical University, Fuzhou, Fujian, ChinaDepartment of Gynaecology, Fujian Provincial Maternity and Children’s Hospital, Fujian Medical University, Fuzhou, Fujian, ChinaDepartment of Gynaecology, Fujian Provincial Maternity and Children’s Hospital, Fujian Medical University, Fuzhou, Fujian, ChinaDepartment of Key Laboratory for Prenatal Diagnosis and Birth Defect, Fujian Provincial Maternity and Children’s Hospital, Fujian Medical University, Fuzhou, Fujian, ChinaDepartment of Laboratory, Fujian Provincial Maternity and Children’s Hospital, Fujian Medical University, Fuzhou, Fujian, ChinaDepartment of Gynaecology, Fujian Provincial Maternity and Children’s Hospital, Fujian Medical University, Fuzhou, Fujian, ChinaDepartment of Obstetrics and Gynecology, Fuzhou Maternity and Child Health care Hospital, Fuzhou, Fujian, ChinaEmbryonic miscarriage severely affects the life quality and physical and mental state of pregnant women. However, the detailed mechanism underlying embryonic miscarriage is not fully understood. This study is aimed at analyzing embryonic miscarriage. We collected samples from 25 normal pregnant women and 25 embryonic miscarriage patients of similar age to analyze microbiota isolated from the vagina. Crude examination of the vagina isolates showed that compared with the control group, 80% of the embryonic miscarriage group contained a significantly lower number of Lactobacillus, the major healthy microbe in the vagina. Furthermore, the levels of Th1 and Th2 secreted cytokine interleukin 2 (IL-2) and interleukin10 (IL-10), respectively, were examined. Results showed that the IL2 level was higher, and IL10 level was lower in the embryonic miscarriage group than in the control group, whereas the IL2/IL10 level was higher in the embryonic miscarriage group than in the control group. This finding suggested that the immune response was suppressed in the embryonic miscarriage group. To further dissect the microbiota of the vagina in the two groups, 16S rDNA sequencing was performed. Bioinformatics analysis showed that 1096 and 998 overlapped operational taxonomic units were identified from the embryonic miscarriage and control groups, respectively. At the genus level, the relative abundance of Fam_Finegoldia, Lac_Coprococcus_3, and Lac_Roseburia significantly differed in the embryonic miscarriage group. Overall, our analyses provided potential biomarkers for embryonic miscarriage and elucidated the causative relationship between microbiota and immune responses and may enable the possible diagnosis and therapeutics of early pregnancy loss.http://dx.doi.org/10.1155/2020/1764959 |
| spellingShingle | Linfen Xu Lingna Huang Chengying Lian Huili Xue Yanfang Lu Xiujuan Chen Yong Xia Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing International Journal of Genomics |
| title | Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing |
| title_full | Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing |
| title_fullStr | Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing |
| title_full_unstemmed | Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing |
| title_short | Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing |
| title_sort | vaginal microbiota diversity of patients with embryonic miscarriage by using 16s rdna high throughput sequencing |
| url | http://dx.doi.org/10.1155/2020/1764959 |
| work_keys_str_mv | AT linfenxu vaginalmicrobiotadiversityofpatientswithembryonicmiscarriagebyusing16srdnahighthroughputsequencing AT lingnahuang vaginalmicrobiotadiversityofpatientswithembryonicmiscarriagebyusing16srdnahighthroughputsequencing AT chengyinglian vaginalmicrobiotadiversityofpatientswithembryonicmiscarriagebyusing16srdnahighthroughputsequencing AT huilixue vaginalmicrobiotadiversityofpatientswithembryonicmiscarriagebyusing16srdnahighthroughputsequencing AT yanfanglu vaginalmicrobiotadiversityofpatientswithembryonicmiscarriagebyusing16srdnahighthroughputsequencing AT xiujuanchen vaginalmicrobiotadiversityofpatientswithembryonicmiscarriagebyusing16srdnahighthroughputsequencing AT yongxia vaginalmicrobiotadiversityofpatientswithembryonicmiscarriagebyusing16srdnahighthroughputsequencing |