Anti-Inflammatory Effects of Concentrated Ethanol Extracts of Edelweiss (Leontopodium alpinum Cass.) Callus Cultures towards Human Keratinocytes and Endothelial Cells
Edelweiss (Leontopodium alpinum Cass.) is traditionally employed in folk medicine as an anti-inflammatory remedy. In nature, the plant is sparsely available and protected; therefore production of callus cultures was established. A concentrated ethanolic extract of culture homogenate, with leontopodi...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/498373 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832564087393878016 |
|---|---|
| author | Lulli Daniela Potapovich Alla Riccardo Maurelli Dellambra Elena Pressi Giovanna Kostyuk Vladimir Dal Toso Roberto De Luca Chiara Pastore Saveria Korkina Liudmila |
| author_facet | Lulli Daniela Potapovich Alla Riccardo Maurelli Dellambra Elena Pressi Giovanna Kostyuk Vladimir Dal Toso Roberto De Luca Chiara Pastore Saveria Korkina Liudmila |
| author_sort | Lulli Daniela |
| collection | DOAJ |
| description | Edelweiss (Leontopodium alpinum Cass.) is traditionally employed in folk medicine as an anti-inflammatory remedy. In nature, the plant is sparsely available and protected; therefore production of callus cultures was established. A concentrated ethanolic extract of culture homogenate, with leontopodic acid representing 55±2% of the total phenolic fraction (ECC55), was characterized for anti-inflammatory properties in primary human keratinocytes (PHKs) and endotheliocytes (HUVECs). Inflammatory responses were induced by UVA+UVB, lipopolysaccharide (LPS), oxidized low-density lipoprotein (oxLDL), and a mixture of proinflammatory cytokines. Trichostatin A, a sirtuin inhibitor, was used to induce keratinocyte inflammatory senescence. ECC55 (10–50 μg/mL) protected PHK from solar UV-driven damage, by enhancing early intracellular levels of nitric oxide, although not affecting UV-induced expression of inflammatory genes. Comparison of the dose-dependent inhibition of chemokine (IL-8, IP-10, MCP-1) and growth factor (GM-CSF) release from PHK activated by TNFα + IFNγ showed that leontopodic acid was mainly responsible for the inhibitory effects of ECC55. Sirtuin-inhibited cell cycle, proliferation, and apoptosis markers were restored by ECC55. The extract inhibited LPS-induced IL-6 and VCAM1 genes in HUVEC, as well as oxLDL-induced selective VCAM1 overexpression. Conclusion. Edelweiss cell cultures could be a valuable source of anti-inflammatory substances potentially applicable for chronic inflammatory skin diseases and bacterial and atherogenic inflammation. |
| format | Article |
| id | doaj-art-cfa9a034f97e4c138eb329b4b8235f4e |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-cfa9a034f97e4c138eb329b4b8235f4e2025-02-03T01:11:57ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/498373498373Anti-Inflammatory Effects of Concentrated Ethanol Extracts of Edelweiss (Leontopodium alpinum Cass.) Callus Cultures towards Human Keratinocytes and Endothelial CellsLulli Daniela0Potapovich Alla1Riccardo Maurelli2Dellambra Elena3Pressi Giovanna4Kostyuk Vladimir5Dal Toso Roberto6De Luca Chiara7Pastore Saveria8Korkina Liudmila9Laboratory of Tissue Engineering and Skin Pathophysiology, Dermatology Institute (IDI IRCCS), Via Monti di Creta 104, 00167 Rome, ItalyLaboratory of Tissue Engineering and Skin Pathophysiology, Dermatology Institute (IDI IRCCS), Via Monti di Creta 104, 00167 Rome, ItalyLaboratory of Tissue Engineering and Skin Pathophysiology, Dermatology Institute (IDI IRCCS), Via Monti di Creta 104, 00167 Rome, ItalyLaboratory of Tissue Engineering and Skin Pathophysiology, Dermatology Institute (IDI IRCCS), Via Monti di Creta 104, 00167 Rome, ItalyInstitute of Biotechnology Research (I.R.B. S.r.l.), Via Lago di Tovel 7, 36077 Altavilla Vicentina, ItalyLaboratory of Tissue Engineering and Skin Pathophysiology, Dermatology Institute (IDI IRCCS), Via Monti di Creta 104, 00167 Rome, ItalyInstitute of Biotechnology Research (I.R.B. S.r.l.), Via Lago di Tovel 7, 36077 Altavilla Vicentina, ItalyLaboratory of Tissue Engineering and Skin Pathophysiology, Dermatology Institute (IDI IRCCS), Via Monti di Creta 104, 00167 Rome, ItalyLaboratory of Tissue Engineering and Skin Pathophysiology, Dermatology Institute (IDI IRCCS), Via Monti di Creta 104, 00167 Rome, ItalyLaboratory of Tissue Engineering and Skin Pathophysiology, Dermatology Institute (IDI IRCCS), Via Monti di Creta 104, 00167 Rome, ItalyEdelweiss (Leontopodium alpinum Cass.) is traditionally employed in folk medicine as an anti-inflammatory remedy. In nature, the plant is sparsely available and protected; therefore production of callus cultures was established. A concentrated ethanolic extract of culture homogenate, with leontopodic acid representing 55±2% of the total phenolic fraction (ECC55), was characterized for anti-inflammatory properties in primary human keratinocytes (PHKs) and endotheliocytes (HUVECs). Inflammatory responses were induced by UVA+UVB, lipopolysaccharide (LPS), oxidized low-density lipoprotein (oxLDL), and a mixture of proinflammatory cytokines. Trichostatin A, a sirtuin inhibitor, was used to induce keratinocyte inflammatory senescence. ECC55 (10–50 μg/mL) protected PHK from solar UV-driven damage, by enhancing early intracellular levels of nitric oxide, although not affecting UV-induced expression of inflammatory genes. Comparison of the dose-dependent inhibition of chemokine (IL-8, IP-10, MCP-1) and growth factor (GM-CSF) release from PHK activated by TNFα + IFNγ showed that leontopodic acid was mainly responsible for the inhibitory effects of ECC55. Sirtuin-inhibited cell cycle, proliferation, and apoptosis markers were restored by ECC55. The extract inhibited LPS-induced IL-6 and VCAM1 genes in HUVEC, as well as oxLDL-induced selective VCAM1 overexpression. Conclusion. Edelweiss cell cultures could be a valuable source of anti-inflammatory substances potentially applicable for chronic inflammatory skin diseases and bacterial and atherogenic inflammation.http://dx.doi.org/10.1155/2012/498373 |
| spellingShingle | Lulli Daniela Potapovich Alla Riccardo Maurelli Dellambra Elena Pressi Giovanna Kostyuk Vladimir Dal Toso Roberto De Luca Chiara Pastore Saveria Korkina Liudmila Anti-Inflammatory Effects of Concentrated Ethanol Extracts of Edelweiss (Leontopodium alpinum Cass.) Callus Cultures towards Human Keratinocytes and Endothelial Cells Mediators of Inflammation |
| title | Anti-Inflammatory Effects of Concentrated Ethanol Extracts of Edelweiss (Leontopodium alpinum Cass.) Callus Cultures towards Human Keratinocytes and Endothelial Cells |
| title_full | Anti-Inflammatory Effects of Concentrated Ethanol Extracts of Edelweiss (Leontopodium alpinum Cass.) Callus Cultures towards Human Keratinocytes and Endothelial Cells |
| title_fullStr | Anti-Inflammatory Effects of Concentrated Ethanol Extracts of Edelweiss (Leontopodium alpinum Cass.) Callus Cultures towards Human Keratinocytes and Endothelial Cells |
| title_full_unstemmed | Anti-Inflammatory Effects of Concentrated Ethanol Extracts of Edelweiss (Leontopodium alpinum Cass.) Callus Cultures towards Human Keratinocytes and Endothelial Cells |
| title_short | Anti-Inflammatory Effects of Concentrated Ethanol Extracts of Edelweiss (Leontopodium alpinum Cass.) Callus Cultures towards Human Keratinocytes and Endothelial Cells |
| title_sort | anti inflammatory effects of concentrated ethanol extracts of edelweiss leontopodium alpinum cass callus cultures towards human keratinocytes and endothelial cells |
| url | http://dx.doi.org/10.1155/2012/498373 |
| work_keys_str_mv | AT lullidaniela antiinflammatoryeffectsofconcentratedethanolextractsofedelweissleontopodiumalpinumcasscallusculturestowardshumankeratinocytesandendothelialcells AT potapovichalla antiinflammatoryeffectsofconcentratedethanolextractsofedelweissleontopodiumalpinumcasscallusculturestowardshumankeratinocytesandendothelialcells AT riccardomaurelli antiinflammatoryeffectsofconcentratedethanolextractsofedelweissleontopodiumalpinumcasscallusculturestowardshumankeratinocytesandendothelialcells AT dellambraelena antiinflammatoryeffectsofconcentratedethanolextractsofedelweissleontopodiumalpinumcasscallusculturestowardshumankeratinocytesandendothelialcells AT pressigiovanna antiinflammatoryeffectsofconcentratedethanolextractsofedelweissleontopodiumalpinumcasscallusculturestowardshumankeratinocytesandendothelialcells AT kostyukvladimir antiinflammatoryeffectsofconcentratedethanolextractsofedelweissleontopodiumalpinumcasscallusculturestowardshumankeratinocytesandendothelialcells AT daltosoroberto antiinflammatoryeffectsofconcentratedethanolextractsofedelweissleontopodiumalpinumcasscallusculturestowardshumankeratinocytesandendothelialcells AT delucachiara antiinflammatoryeffectsofconcentratedethanolextractsofedelweissleontopodiumalpinumcasscallusculturestowardshumankeratinocytesandendothelialcells AT pastoresaveria antiinflammatoryeffectsofconcentratedethanolextractsofedelweissleontopodiumalpinumcasscallusculturestowardshumankeratinocytesandendothelialcells AT korkinaliudmila antiinflammatoryeffectsofconcentratedethanolextractsofedelweissleontopodiumalpinumcasscallusculturestowardshumankeratinocytesandendothelialcells |