Peach Leaf Extract (<i>Prunus persica</i> L.) Mitigates Metabolic Syndrome and Oxidative Stress in High-Fructose Diet Rats

This study aimed to evaluate the protective effects of peach leaf extract (<i>Prunus persica</i> L.) against metabolic syndrome and oxidative stress in <i>Wistar</i> rats subjected to a high-fructose diet. The <i>Wistar</i> rats were divided into groups and fed a...

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Main Authors: Djihane Bali, Zoubida Mami-Soualem, Nabila Belyagoubi-Benhammou, Nassima Benzazoua, Chahrazed Belarbi, Youssouf Kachekouche, Waleed Aldahmash, Md Ataur Rahman, Abdel Halim Harrath
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Language:English
Published: MDPI AG 2025-04-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/9/1332
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author Djihane Bali
Zoubida Mami-Soualem
Nabila Belyagoubi-Benhammou
Nassima Benzazoua
Chahrazed Belarbi
Youssouf Kachekouche
Waleed Aldahmash
Md Ataur Rahman
Abdel Halim Harrath
author_facet Djihane Bali
Zoubida Mami-Soualem
Nabila Belyagoubi-Benhammou
Nassima Benzazoua
Chahrazed Belarbi
Youssouf Kachekouche
Waleed Aldahmash
Md Ataur Rahman
Abdel Halim Harrath
author_sort Djihane Bali
collection DOAJ
description This study aimed to evaluate the protective effects of peach leaf extract (<i>Prunus persica</i> L.) against metabolic syndrome and oxidative stress in <i>Wistar</i> rats subjected to a high-fructose diet. The <i>Wistar</i> rats were divided into groups and fed a high-fructose diet, with or without supplementation of peach leaf extract. The extract was characterized by its bioactive compounds, including an organic acid yield of 53.8%, total phenolic content (TPC) of 273.36 ± 1.929 mg GAE/g DW, flavonoid content (TFC) at 149.02 ± 57.47 mg QE/g DW, condensed tannins (TCT) at 2.34 ± 0.171 mg CE/g DW, and flavonols at 81.67 ± 0.497 mg DE/g DW. In vitro tests showed significant antioxidant potential, with a total antioxidant capacity (TAC) of 44.11 ± 6.328 mg AAE/g DW, DPPH radical scavenging activity (IC<sub>50</sub> = 4.89 mg/mL), and reducing power assay (FRAP, IC<sub>50</sub> = 0.525 mg/mL). The results indicated that the extract significantly reduced body weight gain, plasma insulin levels (0.30 ± 0.00 U(IU)/mL), glycemia (0.955 ± 0.068 g/L), total cholesterol (0.555 ± 0.177 g/L), and triglycerides (0.720 ± 0.141 g/L). Regarding oxidative stress markers, the extract decreased levels of malondialdehyde (MDA, 4567 ± 121 μmol/L), hydroperoxides (1304 ± 288 μmol/L), and carbonylated proteins (0.029 ± 0.020 μmol/L), while increasing levels of vitamin C (25.84 ± 3.00 mg AAE/L), Oxygen Radical Absorbance Capacity (ORAC, 6.043 ± 0.345 UA), and catalase activity (0.0052 ± 0.00008 μL/mL). These findings suggest that <i>P. persica</i> L. may alleviate impairments related to metabolic syndrome by improving metabolic profiles and reducing oxidative stress in rats fed a high-fructose diet, making it a potential dietary supplement for managing metabolic syndrome.
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spelling doaj-art-cf567d016f054c20afb0dc28ecd8f5af2025-08-20T01:49:20ZengMDPI AGPlants2223-77472025-04-01149133210.3390/plants14091332Peach Leaf Extract (<i>Prunus persica</i> L.) Mitigates Metabolic Syndrome and Oxidative Stress in High-Fructose Diet RatsDjihane Bali0Zoubida Mami-Soualem1Nabila Belyagoubi-Benhammou2Nassima Benzazoua3Chahrazed Belarbi4Youssouf Kachekouche5Waleed Aldahmash6Md Ataur Rahman7Abdel Halim Harrath8Natural Products Laboratory, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe Sciences, University Abou-Bekr Belkaïd, Tlemcen 13000, AlgeriaNatural Products Laboratory, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe Sciences, University Abou-Bekr Belkaïd, Tlemcen 13000, AlgeriaNatural Products Laboratory, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe Sciences, University Abou-Bekr Belkaïd, Tlemcen 13000, AlgeriaLaboratory of Histology-Embryology, Faculty of Medicine Dr. Benzerdjeb Benaouda, University of Tlemcen, Tlemcen 13000, AlgeriaNatural Products Laboratory, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe Sciences, University Abou-Bekr Belkaïd, Tlemcen 13000, AlgeriaDepartment of Biology, Faculty of SNV, University of Hassiba Benbouali, Chlef 02000, AlgeriaDepartment of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Oncology, Karmanos Cancer Institute, School of Medicine, Wayne State University, Detroit, MI 48201, USADepartment of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaThis study aimed to evaluate the protective effects of peach leaf extract (<i>Prunus persica</i> L.) against metabolic syndrome and oxidative stress in <i>Wistar</i> rats subjected to a high-fructose diet. The <i>Wistar</i> rats were divided into groups and fed a high-fructose diet, with or without supplementation of peach leaf extract. The extract was characterized by its bioactive compounds, including an organic acid yield of 53.8%, total phenolic content (TPC) of 273.36 ± 1.929 mg GAE/g DW, flavonoid content (TFC) at 149.02 ± 57.47 mg QE/g DW, condensed tannins (TCT) at 2.34 ± 0.171 mg CE/g DW, and flavonols at 81.67 ± 0.497 mg DE/g DW. In vitro tests showed significant antioxidant potential, with a total antioxidant capacity (TAC) of 44.11 ± 6.328 mg AAE/g DW, DPPH radical scavenging activity (IC<sub>50</sub> = 4.89 mg/mL), and reducing power assay (FRAP, IC<sub>50</sub> = 0.525 mg/mL). The results indicated that the extract significantly reduced body weight gain, plasma insulin levels (0.30 ± 0.00 U(IU)/mL), glycemia (0.955 ± 0.068 g/L), total cholesterol (0.555 ± 0.177 g/L), and triglycerides (0.720 ± 0.141 g/L). Regarding oxidative stress markers, the extract decreased levels of malondialdehyde (MDA, 4567 ± 121 μmol/L), hydroperoxides (1304 ± 288 μmol/L), and carbonylated proteins (0.029 ± 0.020 μmol/L), while increasing levels of vitamin C (25.84 ± 3.00 mg AAE/L), Oxygen Radical Absorbance Capacity (ORAC, 6.043 ± 0.345 UA), and catalase activity (0.0052 ± 0.00008 μL/mL). These findings suggest that <i>P. persica</i> L. may alleviate impairments related to metabolic syndrome by improving metabolic profiles and reducing oxidative stress in rats fed a high-fructose diet, making it a potential dietary supplement for managing metabolic syndrome.https://www.mdpi.com/2223-7747/14/9/1332<i>Prunus persica</i> L.phenolic compoundsoxidative stressmetabolic syndromehigh-fructose diet
spellingShingle Djihane Bali
Zoubida Mami-Soualem
Nabila Belyagoubi-Benhammou
Nassima Benzazoua
Chahrazed Belarbi
Youssouf Kachekouche
Waleed Aldahmash
Md Ataur Rahman
Abdel Halim Harrath
Peach Leaf Extract (<i>Prunus persica</i> L.) Mitigates Metabolic Syndrome and Oxidative Stress in High-Fructose Diet Rats
Plants
<i>Prunus persica</i> L.
phenolic compounds
oxidative stress
metabolic syndrome
high-fructose diet
title Peach Leaf Extract (<i>Prunus persica</i> L.) Mitigates Metabolic Syndrome and Oxidative Stress in High-Fructose Diet Rats
title_full Peach Leaf Extract (<i>Prunus persica</i> L.) Mitigates Metabolic Syndrome and Oxidative Stress in High-Fructose Diet Rats
title_fullStr Peach Leaf Extract (<i>Prunus persica</i> L.) Mitigates Metabolic Syndrome and Oxidative Stress in High-Fructose Diet Rats
title_full_unstemmed Peach Leaf Extract (<i>Prunus persica</i> L.) Mitigates Metabolic Syndrome and Oxidative Stress in High-Fructose Diet Rats
title_short Peach Leaf Extract (<i>Prunus persica</i> L.) Mitigates Metabolic Syndrome and Oxidative Stress in High-Fructose Diet Rats
title_sort peach leaf extract i prunus persica i l mitigates metabolic syndrome and oxidative stress in high fructose diet rats
topic <i>Prunus persica</i> L.
phenolic compounds
oxidative stress
metabolic syndrome
high-fructose diet
url https://www.mdpi.com/2223-7747/14/9/1332
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