Changes in Immunoreactivity of Sensory Substances within the Enteric Nervous System of the Porcine Stomach during Experimentally Induced Diabetes
One of the most frequently reported disorders associated with diabetes is gastrointestinal (GI) disturbance. Although pathogenesis of these complications is multifactorial, the complicity of the enteric nervous system (ENS) in this respect has significant importance. Therefore, this paper analysed c...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2018/4735659 |
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| author | Michał Bulc Katarzyna Palus Jarosław Całka Łukasz Zielonka |
| author_facet | Michał Bulc Katarzyna Palus Jarosław Całka Łukasz Zielonka |
| author_sort | Michał Bulc |
| collection | DOAJ |
| description | One of the most frequently reported disorders associated with diabetes is gastrointestinal (GI) disturbance. Although pathogenesis of these complications is multifactorial, the complicity of the enteric nervous system (ENS) in this respect has significant importance. Therefore, this paper analysed changes in substance P- (SP-), calcitonin gene-related peptide- (CGRP-), and leu5-enkephalin- (L-ENK-) like immunoreactivity (LI) in enteric stomach neurons caused by chemically induced diabetes in a porcine model. Using double immunofluorescent labelling, it was found that acute hyperglycaemia led to significant changes in the chemical coding of stomach enteric neurons. Generally, the response to artificially inducted diabetes depended on the “kind” of enteric plexus as well as the stomach region studied. A clear increase in the percentage of neurons immunoreactive to SP and CGRP was visible in the myenteric plexus (MP) in the antrum, corpus, and pylorus as well as in the submucosal plexus (SmP) in the corpus. For L-ENK, an increase in the number of L-ENK-LI neurons was observed in the MP of the antrum and SmP in the corpus, while in the MP of the corpus and pylorus, a decrease in the percentage of L-ENK-LI neurons was noted. |
| format | Article |
| id | doaj-art-cf1ad9da80f84217acdb8649f7c124af |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-cf1ad9da80f84217acdb8649f7c124af2025-02-03T06:05:21ZengWileyJournal of Diabetes Research2314-67452314-67532018-01-01201810.1155/2018/47356594735659Changes in Immunoreactivity of Sensory Substances within the Enteric Nervous System of the Porcine Stomach during Experimentally Induced DiabetesMichał Bulc0Katarzyna Palus1Jarosław Całka2Łukasz Zielonka3Department of Clinical Physiology Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str. 13, 10-719 Olsztyn, PolandDepartment of Clinical Physiology Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str. 13, 10-719 Olsztyn, PolandDepartment of Clinical Physiology Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str. 13, 10-719 Olsztyn, PolandDepartment of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, PolandOne of the most frequently reported disorders associated with diabetes is gastrointestinal (GI) disturbance. Although pathogenesis of these complications is multifactorial, the complicity of the enteric nervous system (ENS) in this respect has significant importance. Therefore, this paper analysed changes in substance P- (SP-), calcitonin gene-related peptide- (CGRP-), and leu5-enkephalin- (L-ENK-) like immunoreactivity (LI) in enteric stomach neurons caused by chemically induced diabetes in a porcine model. Using double immunofluorescent labelling, it was found that acute hyperglycaemia led to significant changes in the chemical coding of stomach enteric neurons. Generally, the response to artificially inducted diabetes depended on the “kind” of enteric plexus as well as the stomach region studied. A clear increase in the percentage of neurons immunoreactive to SP and CGRP was visible in the myenteric plexus (MP) in the antrum, corpus, and pylorus as well as in the submucosal plexus (SmP) in the corpus. For L-ENK, an increase in the number of L-ENK-LI neurons was observed in the MP of the antrum and SmP in the corpus, while in the MP of the corpus and pylorus, a decrease in the percentage of L-ENK-LI neurons was noted.http://dx.doi.org/10.1155/2018/4735659 |
| spellingShingle | Michał Bulc Katarzyna Palus Jarosław Całka Łukasz Zielonka Changes in Immunoreactivity of Sensory Substances within the Enteric Nervous System of the Porcine Stomach during Experimentally Induced Diabetes Journal of Diabetes Research |
| title | Changes in Immunoreactivity of Sensory Substances within the Enteric Nervous System of the Porcine Stomach during Experimentally Induced Diabetes |
| title_full | Changes in Immunoreactivity of Sensory Substances within the Enteric Nervous System of the Porcine Stomach during Experimentally Induced Diabetes |
| title_fullStr | Changes in Immunoreactivity of Sensory Substances within the Enteric Nervous System of the Porcine Stomach during Experimentally Induced Diabetes |
| title_full_unstemmed | Changes in Immunoreactivity of Sensory Substances within the Enteric Nervous System of the Porcine Stomach during Experimentally Induced Diabetes |
| title_short | Changes in Immunoreactivity of Sensory Substances within the Enteric Nervous System of the Porcine Stomach during Experimentally Induced Diabetes |
| title_sort | changes in immunoreactivity of sensory substances within the enteric nervous system of the porcine stomach during experimentally induced diabetes |
| url | http://dx.doi.org/10.1155/2018/4735659 |
| work_keys_str_mv | AT michałbulc changesinimmunoreactivityofsensorysubstanceswithintheentericnervoussystemoftheporcinestomachduringexperimentallyinduceddiabetes AT katarzynapalus changesinimmunoreactivityofsensorysubstanceswithintheentericnervoussystemoftheporcinestomachduringexperimentallyinduceddiabetes AT jarosławcałka changesinimmunoreactivityofsensorysubstanceswithintheentericnervoussystemoftheporcinestomachduringexperimentallyinduceddiabetes AT łukaszzielonka changesinimmunoreactivityofsensorysubstanceswithintheentericnervoussystemoftheporcinestomachduringexperimentallyinduceddiabetes |