Morphology of the Myoepithelial Cell: Immunohistochemical Characterization from Resting to Motile Phase
Myoepithelium is present in canine mammary tumors as resting and proliferative suprabasal and spindle and stellate interstitial cells. The aim of this paper was to evaluate a panel of markers for the identification of four different myoepithelial cell morphological types in the normal and neoplastic...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/252034 |
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| author | Germana Beha Giuseppe Sarli Barbara Brunetti Francesco Sassi Domenico Ferrara Cinzia Benazzi |
| author_facet | Germana Beha Giuseppe Sarli Barbara Brunetti Francesco Sassi Domenico Ferrara Cinzia Benazzi |
| author_sort | Germana Beha |
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| description | Myoepithelium is present in canine mammary tumors as resting and proliferative suprabasal and spindle and stellate interstitial cells. The aim of this paper was to evaluate a panel of markers for the identification of four different myoepithelial cell morphological types in the normal and neoplastic mammary gland and to investigate immunohistochemical changes from an epithelial to a mesenchymal phenotype. Cytokeratin 19 (CK19), cytokeratin 5/6 (CK5/6), cytokeratin 14 (CK14), estrogen receptor (ER), p63 protein, vimentin (VIM), and α-smooth muscle actin (Alpha-SMA) antibodies were used on 29 neoplasms (3 benign and 3 malignant myoepithelial tumors, 7 carcinomas in benign-mixed tumors and 16 complex carcinomas) and on normal tissue of mammary glands. All these antibodies were also tested on 3 mammary tissues from animals with no mammary pathology. The myoepithelial markers were well expressed in the suprabasal cells and gradually lost in the motile types, with the stellate cells maintaining only VIM expression typical of mesenchyma. ER labeled some resting and motile myoepithelial cells. On the basis of our results, we propose a transition from myoepithelial immotile cells into migratory fibroblast-like cells. This transition and the characterization of an immunohistochemical panel for resting and motile myoepithelial cells shed more light on the biological behavior of myoepithelial cells. |
| format | Article |
| id | doaj-art-cebd6dda1fdb4b9fa90773da1cf058b5 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-cebd6dda1fdb4b9fa90773da1cf058b52025-02-03T01:30:01ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/252034252034Morphology of the Myoepithelial Cell: Immunohistochemical Characterization from Resting to Motile PhaseGermana Beha0Giuseppe Sarli1Barbara Brunetti2Francesco Sassi3Domenico Ferrara4Cinzia Benazzi5Department of Veterinary Medical Science, University of Bologna, 40064 Ozzano Emilia, ItalyDepartment of Veterinary Medical Science, University of Bologna, 40064 Ozzano Emilia, ItalyDepartment of Veterinary Medical Science, University of Bologna, 40064 Ozzano Emilia, ItalyInstitute for Cancer Research and Treatment (IRCC), Fourth Floor, 10060 Candiolo, ItalyDepartment of Veterinary Medical Science, University of Bologna, 40064 Ozzano Emilia, ItalyDepartment of Veterinary Medical Science, University of Bologna, 40064 Ozzano Emilia, ItalyMyoepithelium is present in canine mammary tumors as resting and proliferative suprabasal and spindle and stellate interstitial cells. The aim of this paper was to evaluate a panel of markers for the identification of four different myoepithelial cell morphological types in the normal and neoplastic mammary gland and to investigate immunohistochemical changes from an epithelial to a mesenchymal phenotype. Cytokeratin 19 (CK19), cytokeratin 5/6 (CK5/6), cytokeratin 14 (CK14), estrogen receptor (ER), p63 protein, vimentin (VIM), and α-smooth muscle actin (Alpha-SMA) antibodies were used on 29 neoplasms (3 benign and 3 malignant myoepithelial tumors, 7 carcinomas in benign-mixed tumors and 16 complex carcinomas) and on normal tissue of mammary glands. All these antibodies were also tested on 3 mammary tissues from animals with no mammary pathology. The myoepithelial markers were well expressed in the suprabasal cells and gradually lost in the motile types, with the stellate cells maintaining only VIM expression typical of mesenchyma. ER labeled some resting and motile myoepithelial cells. On the basis of our results, we propose a transition from myoepithelial immotile cells into migratory fibroblast-like cells. This transition and the characterization of an immunohistochemical panel for resting and motile myoepithelial cells shed more light on the biological behavior of myoepithelial cells.http://dx.doi.org/10.1100/2012/252034 |
| spellingShingle | Germana Beha Giuseppe Sarli Barbara Brunetti Francesco Sassi Domenico Ferrara Cinzia Benazzi Morphology of the Myoepithelial Cell: Immunohistochemical Characterization from Resting to Motile Phase The Scientific World Journal |
| title | Morphology of the Myoepithelial Cell: Immunohistochemical Characterization from Resting to Motile Phase |
| title_full | Morphology of the Myoepithelial Cell: Immunohistochemical Characterization from Resting to Motile Phase |
| title_fullStr | Morphology of the Myoepithelial Cell: Immunohistochemical Characterization from Resting to Motile Phase |
| title_full_unstemmed | Morphology of the Myoepithelial Cell: Immunohistochemical Characterization from Resting to Motile Phase |
| title_short | Morphology of the Myoepithelial Cell: Immunohistochemical Characterization from Resting to Motile Phase |
| title_sort | morphology of the myoepithelial cell immunohistochemical characterization from resting to motile phase |
| url | http://dx.doi.org/10.1100/2012/252034 |
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