Sequentially Activated Smart DNA Nanospheres for Photoimmunotherapy and Immune Checkpoint Blockade
Abstract Due to the inherent immunosuppression and immune evasion of cancer cells, combining photoimmunotherapy with immune checkpoint blockade leverages phototherapy and immune enhancement, overcoming mutual limitations and demonstrating significant anticancer potential. The main challenges include...
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| Format: | Article |
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Wiley
2025-01-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202410632 |
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| author | Yu Chen Yu Guo Wen Cheng Jiahao Fan Jiacheng Li Jiajia Song Xiaohai Yang Kemin Wang Jin Huang |
| author_facet | Yu Chen Yu Guo Wen Cheng Jiahao Fan Jiacheng Li Jiajia Song Xiaohai Yang Kemin Wang Jin Huang |
| author_sort | Yu Chen |
| collection | DOAJ |
| description | Abstract Due to the inherent immunosuppression and immune evasion of cancer cells, combining photoimmunotherapy with immune checkpoint blockade leverages phototherapy and immune enhancement, overcoming mutual limitations and demonstrating significant anticancer potential. The main challenges include nonspecific accumulation of agents, uncontrolled activation, and drug carrier safety. Smart DNA nanospheres (NS) is developed with targeted delivery and controllable release of photosensitizers and immune agents to achieve effective synergistic therapy and minimize side effects. The multifunctional NS incorporate a targeting module for programming aptamers, a response module for programming i‐motif and DNA/RNA hybrid sequences, and a therapeutic module for packaging photosensitizers and PD‐L1 siRNA. NS navigate to the tumor site and are sequentially activated by intracellular acid and enzymes to release photosensitizers and programmed death ligand 1 (PD‐L1) small interfering RNA (siRNA)a. Besides tumor killing and immune promotion, activated NS downregulate PD‐L1 expression, alleviating immune tolerance and evasion, thus enhancing the immune response. These results indicate that NS significantly enhance antitumor immune responses, synergistically improve antitumor efficacy, and reduce systemic toxicity. This study broadens the application of DNA nanomaterials in precision drug delivery and tumor therapy. |
| format | Article |
| id | doaj-art-cdf1159516a544be8ad19cbd75ddc0e8 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-cdf1159516a544be8ad19cbd75ddc0e82025-01-20T13:04:18ZengWileyAdvanced Science2198-38442025-01-01123n/an/a10.1002/advs.202410632Sequentially Activated Smart DNA Nanospheres for Photoimmunotherapy and Immune Checkpoint BlockadeYu Chen0Yu Guo1Wen Cheng2Jiahao Fan3Jiacheng Li4Jiajia Song5Xiaohai Yang6Kemin Wang7Jin Huang8State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Key Laboratory for Bio‐Nanotechnology and Molecular Engineering of Hunan Province Hunan University Changsha 410082 P. R. ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Key Laboratory for Bio‐Nanotechnology and Molecular Engineering of Hunan Province Hunan University Changsha 410082 P. R. ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Key Laboratory for Bio‐Nanotechnology and Molecular Engineering of Hunan Province Hunan University Changsha 410082 P. R. ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Key Laboratory for Bio‐Nanotechnology and Molecular Engineering of Hunan Province Hunan University Changsha 410082 P. R. ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Key Laboratory for Bio‐Nanotechnology and Molecular Engineering of Hunan Province Hunan University Changsha 410082 P. R. ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Key Laboratory for Bio‐Nanotechnology and Molecular Engineering of Hunan Province Hunan University Changsha 410082 P. R. ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Key Laboratory for Bio‐Nanotechnology and Molecular Engineering of Hunan Province Hunan University Changsha 410082 P. R. ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Key Laboratory for Bio‐Nanotechnology and Molecular Engineering of Hunan Province Hunan University Changsha 410082 P. R. ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Key Laboratory for Bio‐Nanotechnology and Molecular Engineering of Hunan Province Hunan University Changsha 410082 P. R. ChinaAbstract Due to the inherent immunosuppression and immune evasion of cancer cells, combining photoimmunotherapy with immune checkpoint blockade leverages phototherapy and immune enhancement, overcoming mutual limitations and demonstrating significant anticancer potential. The main challenges include nonspecific accumulation of agents, uncontrolled activation, and drug carrier safety. Smart DNA nanospheres (NS) is developed with targeted delivery and controllable release of photosensitizers and immune agents to achieve effective synergistic therapy and minimize side effects. The multifunctional NS incorporate a targeting module for programming aptamers, a response module for programming i‐motif and DNA/RNA hybrid sequences, and a therapeutic module for packaging photosensitizers and PD‐L1 siRNA. NS navigate to the tumor site and are sequentially activated by intracellular acid and enzymes to release photosensitizers and programmed death ligand 1 (PD‐L1) small interfering RNA (siRNA)a. Besides tumor killing and immune promotion, activated NS downregulate PD‐L1 expression, alleviating immune tolerance and evasion, thus enhancing the immune response. These results indicate that NS significantly enhance antitumor immune responses, synergistically improve antitumor efficacy, and reduce systemic toxicity. This study broadens the application of DNA nanomaterials in precision drug delivery and tumor therapy.https://doi.org/10.1002/advs.202410632DNA nanospheresdrug deliveryimmune checkpoint blockadephotoimmunotherapytumor therapy |
| spellingShingle | Yu Chen Yu Guo Wen Cheng Jiahao Fan Jiacheng Li Jiajia Song Xiaohai Yang Kemin Wang Jin Huang Sequentially Activated Smart DNA Nanospheres for Photoimmunotherapy and Immune Checkpoint Blockade Advanced Science DNA nanospheres drug delivery immune checkpoint blockade photoimmunotherapy tumor therapy |
| title | Sequentially Activated Smart DNA Nanospheres for Photoimmunotherapy and Immune Checkpoint Blockade |
| title_full | Sequentially Activated Smart DNA Nanospheres for Photoimmunotherapy and Immune Checkpoint Blockade |
| title_fullStr | Sequentially Activated Smart DNA Nanospheres for Photoimmunotherapy and Immune Checkpoint Blockade |
| title_full_unstemmed | Sequentially Activated Smart DNA Nanospheres for Photoimmunotherapy and Immune Checkpoint Blockade |
| title_short | Sequentially Activated Smart DNA Nanospheres for Photoimmunotherapy and Immune Checkpoint Blockade |
| title_sort | sequentially activated smart dna nanospheres for photoimmunotherapy and immune checkpoint blockade |
| topic | DNA nanospheres drug delivery immune checkpoint blockade photoimmunotherapy tumor therapy |
| url | https://doi.org/10.1002/advs.202410632 |
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