First whole genome report of Mangrovibacter phragmitis PSU-3885–11 isolated from a patient in Thailand
Mangrovibacter phragmitis is a Gram-negative bacterium typically found in plant roots that supports nitrogen fixation in nutrient-poor environments such as mangrove ecosystems. Although primarily found in environmental niches, an unusual case in Thailand of M. phragmitis strain PSU-3885–11 isolated...
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Elsevier
2025-01-01
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| Series: | Current Research in Microbial Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666517425000124 |
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| author | Nattarika Chaichana Thunchanok Yaikhan Mingkwan Yingkajorn Nonthawat Thepsimanon Sirikan Suwannasin Kamonnut Singkhamanan Sarunyou Chusri Rattanaruji Pomwised Monwadee Wonglapsuwan Komwit Surachat |
| author_facet | Nattarika Chaichana Thunchanok Yaikhan Mingkwan Yingkajorn Nonthawat Thepsimanon Sirikan Suwannasin Kamonnut Singkhamanan Sarunyou Chusri Rattanaruji Pomwised Monwadee Wonglapsuwan Komwit Surachat |
| author_sort | Nattarika Chaichana |
| collection | DOAJ |
| description | Mangrovibacter phragmitis is a Gram-negative bacterium typically found in plant roots that supports nitrogen fixation in nutrient-poor environments such as mangrove ecosystems. Although primarily found in environmental niches, an unusual case in Thailand of M. phragmitis strain PSU-3885–11 isolated from the sputum of a 29-year-old female patient with spinal tuberculosis. This isolate was initially misidentified as part of the Enterobacter cloacae complex (ECC) by MALDI-TOF. However, WGS subsequently confirmed its correct identity as M. phragmitis. The genome contains 4,651 coding sequences, along with 72 tRNA genes and 1 tmRNA. Moreover, comparative genomic analysis showed 99.32 % average nucleotide identity (ANI) similar to M. phragmitis MP23, and several antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) were identified in the PSU-3885–11 genome which may contribute to its ability to survive in diverse environments, including human hosts. The PSU-3885–11 displayed resistance to beta-lactam antibiotics such as ampicillin and cefotaxime, while remaining sensitive to a wide range of other antibiotics. Key virulence genes including ompA, hcp/tssD, and rpoS, were identified which may play a role in its persistence in human hosts as an opportunistic pathogen. The presence of ribosomally synthesized and post-translationally modified peptides (RiPPs) and bacteriocins indicates the antimicrobial properties that may provide a competitive advantage in both environmental and clinical settings of this strain. Therefore, this study provides valuable insights into the genomic features, antibiotic resistance, and potential pathogenicity of M. phragmitis PSU-3885–11. The findings also emphasize the importance of continued surveillance and genomic analysis of environmental bacteria that may emerge as opportunistic pathogens in human infections. |
| format | Article |
| id | doaj-art-cd7778e084e24f508d5b6ec2bbf4d44d |
| institution | Kabale University |
| issn | 2666-5174 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Current Research in Microbial Sciences |
| spelling | doaj-art-cd7778e084e24f508d5b6ec2bbf4d44d2025-01-27T04:22:17ZengElsevierCurrent Research in Microbial Sciences2666-51742025-01-018100350First whole genome report of Mangrovibacter phragmitis PSU-3885–11 isolated from a patient in ThailandNattarika Chaichana0Thunchanok Yaikhan1Mingkwan Yingkajorn2Nonthawat Thepsimanon3Sirikan Suwannasin4Kamonnut Singkhamanan5Sarunyou Chusri6Rattanaruji Pomwised7Monwadee Wonglapsuwan8Komwit Surachat9Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, ThailandDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, ThailandDepartment of Pathology, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, ThailandDepartment of Pathology, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, ThailandDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, ThailandDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, ThailandDivision of Infectious Diseases, Department of Internal Medicine, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, ThailandDivision of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla 90110, ThailandDivision of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla 90110, ThailandDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, Thailand; Translational Medicine Research Center, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, Thailand; Corresponding author.Mangrovibacter phragmitis is a Gram-negative bacterium typically found in plant roots that supports nitrogen fixation in nutrient-poor environments such as mangrove ecosystems. Although primarily found in environmental niches, an unusual case in Thailand of M. phragmitis strain PSU-3885–11 isolated from the sputum of a 29-year-old female patient with spinal tuberculosis. This isolate was initially misidentified as part of the Enterobacter cloacae complex (ECC) by MALDI-TOF. However, WGS subsequently confirmed its correct identity as M. phragmitis. The genome contains 4,651 coding sequences, along with 72 tRNA genes and 1 tmRNA. Moreover, comparative genomic analysis showed 99.32 % average nucleotide identity (ANI) similar to M. phragmitis MP23, and several antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) were identified in the PSU-3885–11 genome which may contribute to its ability to survive in diverse environments, including human hosts. The PSU-3885–11 displayed resistance to beta-lactam antibiotics such as ampicillin and cefotaxime, while remaining sensitive to a wide range of other antibiotics. Key virulence genes including ompA, hcp/tssD, and rpoS, were identified which may play a role in its persistence in human hosts as an opportunistic pathogen. The presence of ribosomally synthesized and post-translationally modified peptides (RiPPs) and bacteriocins indicates the antimicrobial properties that may provide a competitive advantage in both environmental and clinical settings of this strain. Therefore, this study provides valuable insights into the genomic features, antibiotic resistance, and potential pathogenicity of M. phragmitis PSU-3885–11. The findings also emphasize the importance of continued surveillance and genomic analysis of environmental bacteria that may emerge as opportunistic pathogens in human infections.http://www.sciencedirect.com/science/article/pii/S2666517425000124Whole genome sequencingMangrovibacter phragmitisDraft genomeBioinformaticsAntibiotic resistance |
| spellingShingle | Nattarika Chaichana Thunchanok Yaikhan Mingkwan Yingkajorn Nonthawat Thepsimanon Sirikan Suwannasin Kamonnut Singkhamanan Sarunyou Chusri Rattanaruji Pomwised Monwadee Wonglapsuwan Komwit Surachat First whole genome report of Mangrovibacter phragmitis PSU-3885–11 isolated from a patient in Thailand Current Research in Microbial Sciences Whole genome sequencing Mangrovibacter phragmitis Draft genome Bioinformatics Antibiotic resistance |
| title | First whole genome report of Mangrovibacter phragmitis PSU-3885–11 isolated from a patient in Thailand |
| title_full | First whole genome report of Mangrovibacter phragmitis PSU-3885–11 isolated from a patient in Thailand |
| title_fullStr | First whole genome report of Mangrovibacter phragmitis PSU-3885–11 isolated from a patient in Thailand |
| title_full_unstemmed | First whole genome report of Mangrovibacter phragmitis PSU-3885–11 isolated from a patient in Thailand |
| title_short | First whole genome report of Mangrovibacter phragmitis PSU-3885–11 isolated from a patient in Thailand |
| title_sort | first whole genome report of mangrovibacter phragmitis psu 3885 11 isolated from a patient in thailand |
| topic | Whole genome sequencing Mangrovibacter phragmitis Draft genome Bioinformatics Antibiotic resistance |
| url | http://www.sciencedirect.com/science/article/pii/S2666517425000124 |
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