靶向调控非小细胞肺癌的作用机制
【目的】探究miR-204-3p 靶向EphB2 基因对非小细胞肺癌增殖、凋亡、侵袭的调控机制。【方法】培养将非小细胞肺癌A549 细胞系分为 5 组 NC mimic 组(miR-204-3p 过表达阴性对照组)、miR-204-3p mimic 组(miR-204-3p 过表达组)、oe-NC 组(EphB2 过表达阴性对照组)、oe-EphB2 组(EphB2 过表达组)、miR-204-3p mimic+oe-EphB2 组(miR-204-3p 与EphB2 均过表达组)。MTT、流式细胞术、Transwell、划痕实验分别检测细胞增殖、周期、凋亡、侵袭、迁移情况。双荧光素酶报告分析...
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Main Authors: | , , , , , , , |
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Format: | Article |
Language: | zho |
Published: |
Editorial Office of Journal of Sun Yat-sen University
2020-01-01
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Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
Subjects: | |
Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/43723543/ |
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