microRNA-320d通过PBX3抑制子宫内膜癌JEC细胞上皮-间质转化功能

【目的】研究microRNA-320d(miR-320d)对子宫内膜癌JEC细胞上皮-间质转化功能的影响及其相关机制。【方 法】 JEC子宫内膜癌细胞株分别转染miR-320d mimics和negative control mimic,分别作为M320d、NCM组,并设立未转染对 照control组,采用RT-PCR法检测各组细胞miR-320d含量。Transwell实验检测3组细胞迁移能力和侵袭能力。Western- blot法检测3组细胞α-Catenin和PBX3表达水平,及PBX3过表达对miR-320d抑制EMT的拮抗作用。双荧光素酶实验检测 miR-320d与PBX3的...

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Bibliographic Details
Main Authors: 龚凤球, 姚书忠
Format: Article
Language:zho
Published: Editorial Office of Journal of Sun Yat-sen University 2017-01-01
Series:Zhongshan Daxue xuebao. Yixue kexue ban
Subjects:
Online Access:http://xuebaoyx.sysu.edu.cn/zh/article/43567995/
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