Isolation, Identification, and Pathogenicity of Potential Probiotics Isolated from Intestinal Coconut Crab (Birgus latro Linnaeus, 1767)
Abstract The supply of coconut crabs (Birgus latro Linnaeus, 1767) has been sourced from capture in the wild. Development efforts in aquaculture have succeeded in producing eggs. However, further development with larval maintenance failed because it was constrained by a low survival rate. This stu...
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Faculty of Fisheries and Marine Universitas Airlangga
2023-11-01
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| Series: | Jurnal Ilmiah Perikanan dan Kelautan |
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| Online Access: | https://e-journal.unair.ac.id/JIPK/article/view/45647 |
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| author | Muhammad Aris Tamrin Tamrin Waode Munaeni Sudirto Malan Juharni Juharni Rusmawati Labenua |
| author_facet | Muhammad Aris Tamrin Tamrin Waode Munaeni Sudirto Malan Juharni Juharni Rusmawati Labenua |
| author_sort | Muhammad Aris |
| collection | DOAJ |
| description | Abstract
The supply of coconut crabs (Birgus latro Linnaeus, 1767) has been sourced from capture in the wild. Development efforts in aquaculture have succeeded in producing eggs. However, further development with larval maintenance failed because it was constrained by a low survival rate. This study aimed to identify the potential probiotic bacteria in coconut crabs. Coconut crab samples were collected from Moor Island, South Patani District, Central Halmahera Regency, North Maluku Province, Indonesia. The intestines of coconut crabs were taken as much as 1 g, then ground on a porcelain dish and added to Tri-Salt. The scouring liquid was taken in 0.1 ml and spread on a petri dish containing Nutrient Agar. The isolated used were marked with BL1, BL2, BL3, BL4, and BL5. Molecular identification was performed with sequencing and then analyzed using the Basic Local Alignment Search Tool (BLAST). The selection of isolates from the gastrointestinal tract of coconut crabs showed that the bacteria obtained were Enterobacter tabaci (BL1), Enterobacter hormaechei (BL2), Bacillus horneckiae (BL3), Pseudomonas stutzeri (BL4), and Acinetobacter variabilis (BL5). Further testing revealed that the bacteria A. variabilis (BL5) is a probiotic isolate in coconut crabs.
Highlight Research
• The intestinal bacteria from coconut crabs identified were E. tabaci, E. hormaechei, B. horneckiae, P. stutzeri, and A. variabilis.
• The E. tabaci strain BL1, E. hormaechei strain BL2, B. Horneckiae strain BL3, and P. stutzeri strain BL4 are pathogenic bacteria.
• The A. variabilis strain BL5 is potential probiotics bacteria.
• The A. variabilis strain BL5 showed a better survival rate than other isolates. |
| format | Article |
| id | doaj-art-cbc32b9f9c734de59aa7e739a457f90c |
| institution | Kabale University |
| issn | 2085-5842 2528-0759 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Faculty of Fisheries and Marine Universitas Airlangga |
| record_format | Article |
| series | Jurnal Ilmiah Perikanan dan Kelautan |
| spelling | doaj-art-cbc32b9f9c734de59aa7e739a457f90c2025-02-03T05:30:46ZengFaculty of Fisheries and Marine Universitas AirlanggaJurnal Ilmiah Perikanan dan Kelautan2085-58422528-07592023-11-0116124525410.20473/jipk.v16i1.4564743732Isolation, Identification, and Pathogenicity of Potential Probiotics Isolated from Intestinal Coconut Crab (Birgus latro Linnaeus, 1767)Muhammad Aris0https://orcid.org/0000-0001-7724-4175Tamrin Tamrin1Waode Munaeni2Sudirto Malan3Juharni Juharni4Rusmawati Labenua5Department of Aquaculture, Faculty of Fisheries and Marine Science, Khairun University, Ternate City, North Maluku Province, 97719. IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, Khairun University, Ternate City, North Maluku Province, 97719. IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, Khairun University, Ternate City, North Maluku Province, 97719. IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, Khairun University, Ternate City, North Maluku Province, 97719. IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, Khairun University, Ternate City, North Maluku Province, 97719. IndonesiaDepartment of Aquatic Resource Management, Faculty of Fisheries and Marine Science, Khairun University, Ternate City, North Maluku Province, 97719. IndonesiaAbstract The supply of coconut crabs (Birgus latro Linnaeus, 1767) has been sourced from capture in the wild. Development efforts in aquaculture have succeeded in producing eggs. However, further development with larval maintenance failed because it was constrained by a low survival rate. This study aimed to identify the potential probiotic bacteria in coconut crabs. Coconut crab samples were collected from Moor Island, South Patani District, Central Halmahera Regency, North Maluku Province, Indonesia. The intestines of coconut crabs were taken as much as 1 g, then ground on a porcelain dish and added to Tri-Salt. The scouring liquid was taken in 0.1 ml and spread on a petri dish containing Nutrient Agar. The isolated used were marked with BL1, BL2, BL3, BL4, and BL5. Molecular identification was performed with sequencing and then analyzed using the Basic Local Alignment Search Tool (BLAST). The selection of isolates from the gastrointestinal tract of coconut crabs showed that the bacteria obtained were Enterobacter tabaci (BL1), Enterobacter hormaechei (BL2), Bacillus horneckiae (BL3), Pseudomonas stutzeri (BL4), and Acinetobacter variabilis (BL5). Further testing revealed that the bacteria A. variabilis (BL5) is a probiotic isolate in coconut crabs. Highlight Research • The intestinal bacteria from coconut crabs identified were E. tabaci, E. hormaechei, B. horneckiae, P. stutzeri, and A. variabilis. • The E. tabaci strain BL1, E. hormaechei strain BL2, B. Horneckiae strain BL3, and P. stutzeri strain BL4 are pathogenic bacteria. • The A. variabilis strain BL5 is potential probiotics bacteria. • The A. variabilis strain BL5 showed a better survival rate than other isolates.https://e-journal.unair.ac.id/JIPK/article/view/45647acinetobacter variabilisbirgus latrocrustaceansprobiotic |
| spellingShingle | Muhammad Aris Tamrin Tamrin Waode Munaeni Sudirto Malan Juharni Juharni Rusmawati Labenua Isolation, Identification, and Pathogenicity of Potential Probiotics Isolated from Intestinal Coconut Crab (Birgus latro Linnaeus, 1767) Jurnal Ilmiah Perikanan dan Kelautan acinetobacter variabilis birgus latro crustaceans probiotic |
| title | Isolation, Identification, and Pathogenicity of Potential Probiotics Isolated from Intestinal Coconut Crab (Birgus latro Linnaeus, 1767) |
| title_full | Isolation, Identification, and Pathogenicity of Potential Probiotics Isolated from Intestinal Coconut Crab (Birgus latro Linnaeus, 1767) |
| title_fullStr | Isolation, Identification, and Pathogenicity of Potential Probiotics Isolated from Intestinal Coconut Crab (Birgus latro Linnaeus, 1767) |
| title_full_unstemmed | Isolation, Identification, and Pathogenicity of Potential Probiotics Isolated from Intestinal Coconut Crab (Birgus latro Linnaeus, 1767) |
| title_short | Isolation, Identification, and Pathogenicity of Potential Probiotics Isolated from Intestinal Coconut Crab (Birgus latro Linnaeus, 1767) |
| title_sort | isolation identification and pathogenicity of potential probiotics isolated from intestinal coconut crab birgus latro linnaeus 1767 |
| topic | acinetobacter variabilis birgus latro crustaceans probiotic |
| url | https://e-journal.unair.ac.id/JIPK/article/view/45647 |
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