Human Mast Cells (HMC-1 5C6) Enhance Interleukin-6 Production by Quiescent and Lipopolysaccharide-Stimulated Human Coronary Artery Endothelial Cells
We examined the effect of intact human mast cells (HMC-1 5C6) and their selected mediators on interleukin-6 (IL-6) production and bone morphogenetic protein-2 (BMP-2) expression in human coronary artery endothelial cells (HCAEC) in the presence and absence of lipopolysaccharide (LPS). Scanning elect...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/274347 |
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| author | Damandeep S. Walia Mukut Sharma Vineesh V. Raveendran Jianping Zhou Ram Sharma Daniel J. Stechschulte Kottarappat N. Dileepan |
| author_facet | Damandeep S. Walia Mukut Sharma Vineesh V. Raveendran Jianping Zhou Ram Sharma Daniel J. Stechschulte Kottarappat N. Dileepan |
| author_sort | Damandeep S. Walia |
| collection | DOAJ |
| description | We examined the effect of intact human mast cells (HMC-1 5C6) and their selected mediators on interleukin-6 (IL-6) production and bone morphogenetic protein-2 (BMP-2) expression in human coronary artery endothelial cells (HCAEC) in the presence and absence of lipopolysaccharide (LPS). Scanning electron microscopy showed that HMC-1 5C6 cells adhere to HCAEC in cocultures. Addition of HMC-1 5C6 cells markedly enhanced the IL-6 production by quiescent and LPS-activated HCAEC even at the maximal concentration of LPS. Furthermore, mast cell-derived histamine and proteases accounted for the direct and synergistic effect of mast cells on IL-6 production that was completely blocked by the combination of histamine receptor-1 antagonist and protease inhibitors. Another novel finding is that histamine was able to induce BMP-2 expression in HCAEC. Collectively, our results suggest that endotoxin and mast cell products synergistically amplify vascular inflammation and that histamine participates in the early events of vascular calcification. |
| format | Article |
| id | doaj-art-cb0ceae9e5994d84a5245abfb18ce4c6 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-cb0ceae9e5994d84a5245abfb18ce4c62025-02-03T06:08:23ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/274347274347Human Mast Cells (HMC-1 5C6) Enhance Interleukin-6 Production by Quiescent and Lipopolysaccharide-Stimulated Human Coronary Artery Endothelial CellsDamandeep S. Walia0Mukut Sharma1Vineesh V. Raveendran2Jianping Zhou3Ram Sharma4Daniel J. Stechschulte5Kottarappat N. Dileepan6Division of Allergy, Clinical Immunology, and Rheumatology, Department of Internal Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USAKansas City, Veterans Affairs Medical Center, Kansas City, MO 64128, USADivision of Allergy, Clinical Immunology, and Rheumatology, Department of Internal Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USAKansas City, Veterans Affairs Medical Center, Kansas City, MO 64128, USAKansas City, Veterans Affairs Medical Center, Kansas City, MO 64128, USADivision of Allergy, Clinical Immunology, and Rheumatology, Department of Internal Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USADivision of Allergy, Clinical Immunology, and Rheumatology, Department of Internal Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USAWe examined the effect of intact human mast cells (HMC-1 5C6) and their selected mediators on interleukin-6 (IL-6) production and bone morphogenetic protein-2 (BMP-2) expression in human coronary artery endothelial cells (HCAEC) in the presence and absence of lipopolysaccharide (LPS). Scanning electron microscopy showed that HMC-1 5C6 cells adhere to HCAEC in cocultures. Addition of HMC-1 5C6 cells markedly enhanced the IL-6 production by quiescent and LPS-activated HCAEC even at the maximal concentration of LPS. Furthermore, mast cell-derived histamine and proteases accounted for the direct and synergistic effect of mast cells on IL-6 production that was completely blocked by the combination of histamine receptor-1 antagonist and protease inhibitors. Another novel finding is that histamine was able to induce BMP-2 expression in HCAEC. Collectively, our results suggest that endotoxin and mast cell products synergistically amplify vascular inflammation and that histamine participates in the early events of vascular calcification.http://dx.doi.org/10.1155/2012/274347 |
| spellingShingle | Damandeep S. Walia Mukut Sharma Vineesh V. Raveendran Jianping Zhou Ram Sharma Daniel J. Stechschulte Kottarappat N. Dileepan Human Mast Cells (HMC-1 5C6) Enhance Interleukin-6 Production by Quiescent and Lipopolysaccharide-Stimulated Human Coronary Artery Endothelial Cells Mediators of Inflammation |
| title | Human Mast Cells (HMC-1 5C6) Enhance Interleukin-6 Production by Quiescent and Lipopolysaccharide-Stimulated Human Coronary Artery Endothelial Cells |
| title_full | Human Mast Cells (HMC-1 5C6) Enhance Interleukin-6 Production by Quiescent and Lipopolysaccharide-Stimulated Human Coronary Artery Endothelial Cells |
| title_fullStr | Human Mast Cells (HMC-1 5C6) Enhance Interleukin-6 Production by Quiescent and Lipopolysaccharide-Stimulated Human Coronary Artery Endothelial Cells |
| title_full_unstemmed | Human Mast Cells (HMC-1 5C6) Enhance Interleukin-6 Production by Quiescent and Lipopolysaccharide-Stimulated Human Coronary Artery Endothelial Cells |
| title_short | Human Mast Cells (HMC-1 5C6) Enhance Interleukin-6 Production by Quiescent and Lipopolysaccharide-Stimulated Human Coronary Artery Endothelial Cells |
| title_sort | human mast cells hmc 1 5c6 enhance interleukin 6 production by quiescent and lipopolysaccharide stimulated human coronary artery endothelial cells |
| url | http://dx.doi.org/10.1155/2012/274347 |
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