A differentiation protocol for generating pancreatic delta cells from human pluripotent stem cells

A differentiation protocol for generating pancreatic delta cells from human pluripotent stem cells

In this protocol, we detail a seven-stage differentiation methodology for generating pancreatic delta cells (SC-delta cells) from human pluripotent stem cells (hPSCs). In the first step, definitive endoderm is generated by activin A and CHIR99021, followed by induction of primitive gut tube and post...

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Bibliographic Details
Main Authors: Tongran Zhang, Nannan Wang, Zhiying Liao, Jingyi Chen, Hao Meng, Haopeng Lin, Tao Xu, Lihua Chen, Ling-Qiang Zhu, Huisheng Liu
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-10-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
hPSCs
stem cell differentiation
pancreatic delta cells
FGF
islet organoids
Online Access:https://www.frontiersin.org/articles/10.3389/fcell.2024.1490040/full
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