One-pot synthesis and characterization of a BSA-Cu2S/CuS quantum dots drug delivery system based on biuret’s reaction
Research on chalcogenide-based quantum dots (QDs) has advanced significantly over recent decades, largely due to their excellent optical properties, particularly photoluminescence, and relatively low toxicity. Among these, Cu _2 S and CuS quantum dots have demonstrated notable photothermal effects a...
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| Format: | Article |
| Language: | English |
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IOP Publishing
2025-01-01
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| Series: | Materials Research Express |
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| Online Access: | https://doi.org/10.1088/2053-1591/ada5b0 |
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| author | Porfirio Estrada-Rojas Juan Francisco Hernández-Paz Claudia Alejandra Rodríguez-González Mónica Elvira Mendoza-Duarte Santos Adriana Martel-Estrada Laura Elizabeth Valencia-Gómez Héctor Camacho-Montes Imelda Olivas-Armendáriz |
| author_facet | Porfirio Estrada-Rojas Juan Francisco Hernández-Paz Claudia Alejandra Rodríguez-González Mónica Elvira Mendoza-Duarte Santos Adriana Martel-Estrada Laura Elizabeth Valencia-Gómez Héctor Camacho-Montes Imelda Olivas-Armendáriz |
| author_sort | Porfirio Estrada-Rojas |
| collection | DOAJ |
| description | Research on chalcogenide-based quantum dots (QDs) has advanced significantly over recent decades, largely due to their excellent optical properties, particularly photoluminescence, and relatively low toxicity. Among these, Cu _2 S and CuS quantum dots have demonstrated notable photothermal effects and antibacterial activity, making them promising candidates for various biomedical applications, including cancer treatment. This study focuses on the synthesis of CuS and Cu _2 S quantum dots functionalized with bovine serum albumin (BSA), marking an important step toward their potential biomedical use. The functionalization was achieved through a one-pot synthesis method, characterized by its ability to offer higher chemical yield, elimination of toxic solvents, and provide a method that is simple, economical, and well-controlled. This synthesis produced four samples with varying BSA concentrations. Due to the lower protein concentration, which provides fewer nucleation sites, sample A (BSA concentration 5 mg ml ^−1 ) showed a particle size distribution over 100 nm and low stability over time. Sample B (25 mg ml ^−1 BSA) and C (50 mg ml ^−1 BSA) showed excellent stability and particle sizes within the expected range for Cu _2 S and CuS quantum dots (<2.4 nm). Sample D was synthesized with lower BSA concentration (2.5 mg ml ^−1 ) and was used only for comparison in few analyses. Fourier-transform infrared (FTIR) spectroscopy confirmed the structural integrity of BSA after functionalization, while x-ray diffraction (XRD) analysis verified the formation of CuS and Cu _2 S crystals. Additional tests, including fluorescence and UV–vis spectroscopy, validated the optical properties of the quantum dots. Transmission electron microscopy (TEM) confirmed crystalline structure of the CuS and Cu _2 S quantum dots, with particle size measurements aligning with expectations. Finally, MTT assays confirmed that the synthesized quantum dots are non-toxic, further supporting their potential for biomedical applications. |
| format | Article |
| id | doaj-art-cab4208dc615490f99c0faa96ed66165 |
| institution | Kabale University |
| issn | 2053-1591 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | IOP Publishing |
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| series | Materials Research Express |
| spelling | doaj-art-cab4208dc615490f99c0faa96ed661652025-01-22T20:15:30ZengIOP PublishingMaterials Research Express2053-15912025-01-0112101540610.1088/2053-1591/ada5b0One-pot synthesis and characterization of a BSA-Cu2S/CuS quantum dots drug delivery system based on biuret’s reactionPorfirio Estrada-Rojas0https://orcid.org/0000-0002-1583-9533Juan Francisco Hernández-Paz1https://orcid.org/0000-0002-1621-835XClaudia Alejandra Rodríguez-González2https://orcid.org/0000-0002-1820-1893Mónica Elvira Mendoza-Duarte3https://orcid.org/0000-0002-9614-2016Santos Adriana Martel-Estrada4https://orcid.org/0000-0002-8803-9406Laura Elizabeth Valencia-Gómez5https://orcid.org/0000-0002-2254-8013Héctor Camacho-Montes6https://orcid.org/0000-0002-2241-9545Imelda Olivas-Armendáriz7https://orcid.org/0000-0003-2233-0310Instituto de Ingeniería y Tecnología, Universidad Autónoma de Ciudad Juárez , Chih., MexicoInstituto de Ingeniería y Tecnología, Universidad Autónoma de Ciudad Juárez , Chih., MexicoInstituto de Ingeniería y Tecnología, Universidad Autónoma de Ciudad Juárez , Chih., MexicoCentro de Investigación en Materiales Avanzados, Chihuahua, Chih., MexicoInstituto de Arquitectura, Diseño y Arte, Universidad Autónoma de Ciudad Juárez , Chih., MexicoInstituto de Ingeniería y Tecnología, Universidad Autónoma de Ciudad Juárez , Chih., MexicoInstituto de Ingeniería y Tecnología, Universidad Autónoma de Ciudad Juárez , Chih., MexicoInstituto de Ingeniería y Tecnología, Universidad Autónoma de Ciudad Juárez , Chih., MexicoResearch on chalcogenide-based quantum dots (QDs) has advanced significantly over recent decades, largely due to their excellent optical properties, particularly photoluminescence, and relatively low toxicity. Among these, Cu _2 S and CuS quantum dots have demonstrated notable photothermal effects and antibacterial activity, making them promising candidates for various biomedical applications, including cancer treatment. This study focuses on the synthesis of CuS and Cu _2 S quantum dots functionalized with bovine serum albumin (BSA), marking an important step toward their potential biomedical use. The functionalization was achieved through a one-pot synthesis method, characterized by its ability to offer higher chemical yield, elimination of toxic solvents, and provide a method that is simple, economical, and well-controlled. This synthesis produced four samples with varying BSA concentrations. Due to the lower protein concentration, which provides fewer nucleation sites, sample A (BSA concentration 5 mg ml ^−1 ) showed a particle size distribution over 100 nm and low stability over time. Sample B (25 mg ml ^−1 BSA) and C (50 mg ml ^−1 BSA) showed excellent stability and particle sizes within the expected range for Cu _2 S and CuS quantum dots (<2.4 nm). Sample D was synthesized with lower BSA concentration (2.5 mg ml ^−1 ) and was used only for comparison in few analyses. Fourier-transform infrared (FTIR) spectroscopy confirmed the structural integrity of BSA after functionalization, while x-ray diffraction (XRD) analysis verified the formation of CuS and Cu _2 S crystals. Additional tests, including fluorescence and UV–vis spectroscopy, validated the optical properties of the quantum dots. Transmission electron microscopy (TEM) confirmed crystalline structure of the CuS and Cu _2 S quantum dots, with particle size measurements aligning with expectations. Finally, MTT assays confirmed that the synthesized quantum dots are non-toxic, further supporting their potential for biomedical applications.https://doi.org/10.1088/2053-1591/ada5b0one-pot synthesisCu2SCuSquantum dotsBSA |
| spellingShingle | Porfirio Estrada-Rojas Juan Francisco Hernández-Paz Claudia Alejandra Rodríguez-González Mónica Elvira Mendoza-Duarte Santos Adriana Martel-Estrada Laura Elizabeth Valencia-Gómez Héctor Camacho-Montes Imelda Olivas-Armendáriz One-pot synthesis and characterization of a BSA-Cu2S/CuS quantum dots drug delivery system based on biuret’s reaction Materials Research Express one-pot synthesis Cu2S CuS quantum dots BSA |
| title | One-pot synthesis and characterization of a BSA-Cu2S/CuS quantum dots drug delivery system based on biuret’s reaction |
| title_full | One-pot synthesis and characterization of a BSA-Cu2S/CuS quantum dots drug delivery system based on biuret’s reaction |
| title_fullStr | One-pot synthesis and characterization of a BSA-Cu2S/CuS quantum dots drug delivery system based on biuret’s reaction |
| title_full_unstemmed | One-pot synthesis and characterization of a BSA-Cu2S/CuS quantum dots drug delivery system based on biuret’s reaction |
| title_short | One-pot synthesis and characterization of a BSA-Cu2S/CuS quantum dots drug delivery system based on biuret’s reaction |
| title_sort | one pot synthesis and characterization of a bsa cu2s cus quantum dots drug delivery system based on biuret s reaction |
| topic | one-pot synthesis Cu2S CuS quantum dots BSA |
| url | https://doi.org/10.1088/2053-1591/ada5b0 |
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