Interlaboratory validation trial report on multiplex real-time PCR method for molecular serotyping and identification of the 30 major clonal complexes of Listeria monocytogenes circulating in food in Europe

Interlaboratory validation trial report on multiplex real-time PCR method for molecular serotyping and identification of the 30 major clonal complexes of Listeria monocytogenes circulating in food in Europe

ABSTRACT The performance of a new method developed in 2021 by the European Union Reference Laboratory (EURL) for Listeria monocytogenes based on 12 multiplex real-time PCR, allowing the identification of the molecular serotype and the 30 major L. monocytogenes multilocus sequence typing clonal compl...

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Main Authors: Karine Capitaine, Sandrine Te, Adrien Asséré, Hana Plodková, Valerie Michel, Pauline Sabrou, Erwan Bourdonnais, Guillaume Gillot, Nassim Mouhali, Thomas Brauge, Cécile Dumaire, Carole Feurer, Baptiste Houry, Stefanie Lueth, Zsuzsanna Sréterné Lancz, Gabriella Centorotola, Fabrizia Guidi, Marina Torresi, Tone Mathisen Fagereng, Taran Skjerdal, Hugo Guedes, Gonçalo Nieto Almeida, Laurentiu Mihai Ciupescu, Paula Ågren, Monica Ricão, Elisabet Marti, Wilma Jacobs-Reitsma, Angela van Hoek, Benjamin Félix
Format: Article
Language:English
Published: American Society for Microbiology 2025-07-01
Series:Microbiology Spectrum
Subjects:
Listeria monocytogenes
real-time PCR
clonal complex
MLST
molecular serotype
interlaboratory validation trial
Online Access:https://journals.asm.org/doi/10.1128/spectrum.00116-25
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https://journals.asm.org/doi/10.1128/spectrum.00116-25

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