Optimization and characterization of bioactive secondary metabolites from Streptomyces sp CMSTAAHL-4 isolated from mangrove sediment
Abstract Background Ten morphologically different actinomycetes were isolated from mangrove sediments of Manakudy, Kanyakumari District, India. The potent strain was selected based on their primary screening against Gram positive Staphylococcus aureus, Enterococcus faecalis and Gram negative Klebsie...
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2025-01-01
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| author | Selvaraj Jeraldin Nisha Ganapathi Uma Ramamoorthy Sathishkumar Vincent Samuel Gnana Prakash Rimal Isaac Thavasimuthu Citarasu |
| author_facet | Selvaraj Jeraldin Nisha Ganapathi Uma Ramamoorthy Sathishkumar Vincent Samuel Gnana Prakash Rimal Isaac Thavasimuthu Citarasu |
| author_sort | Selvaraj Jeraldin Nisha |
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| description | Abstract Background Ten morphologically different actinomycetes were isolated from mangrove sediments of Manakudy, Kanyakumari District, India. The potent strain was selected based on their primary screening against Gram positive Staphylococcus aureus, Enterococcus faecalis and Gram negative Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi bacterial pathogens. The selected strain was identified as Streptomyces sp CMSTAAHL-4 by 16S rRNA sequencing. The media optimization for secondary metabolites production was performed by One-Variable at a Time and Response Surface Methodology-Central Composite Design. Minimum inhibitory concentration and minimum bacterial concentration for the extracted secondary metabolites were determined. The antioxidant potential of the secondary metabolites showed that the concentration of the metabolites increases, with the percentage of inhibition. The anti-inflammatory activity of the secondary metabolites found that maximum activity was observed at 500 µg/ml of the metabolites. Alcohols, alkenes, alkynes, alkyl halides, carboxylic acids, aliphatic esters functional groups were identified by fourier transform infrared spectroscopy, gas chromatography and mass spectrometer analysis of the secondary metabolites revealed five bioactive compounds. The X-ray diffraction analysis revealed that the secondary metabolites are amorphous. The thermogravimetric analysis showed the thermal stability of secondary metabolites. Atomic force microscopy analysis revealed specific structural characteristics of the secondary metabolites, which may be associated with their potential biological activities. Conclusions The results showed that the antibacterial, antioxidant, and anti-inflammatory chemicals present in the isolated secondary metabolites give them therapeutic properties. |
| format | Article |
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| institution | Kabale University |
| issn | 1471-2180 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-ca2ff0bc7ea24d2ea2c9e1945d9a19ef2025-02-02T12:11:17ZengBMCBMC Microbiology1471-21802025-01-0125112110.1186/s12866-025-03763-5Optimization and characterization of bioactive secondary metabolites from Streptomyces sp CMSTAAHL-4 isolated from mangrove sedimentSelvaraj Jeraldin Nisha0Ganapathi Uma1Ramamoorthy Sathishkumar2Vincent Samuel Gnana Prakash3Rimal Isaac4Thavasimuthu Citarasu5Centre for Marine Science and Technology (CMST), Manonmaniam Sundaranar UniversityCentre for Marine Science and Technology (CMST), Manonmaniam Sundaranar UniversityCentre for Marine Science and Technology (CMST), Manonmaniam Sundaranar UniversityCentre for Marine Science and Technology (CMST), Manonmaniam Sundaranar UniversityDepartment of Nanotechnology, Noorul Islam Centre for Higher EducationCentre for Marine Science and Technology (CMST), Manonmaniam Sundaranar UniversityAbstract Background Ten morphologically different actinomycetes were isolated from mangrove sediments of Manakudy, Kanyakumari District, India. The potent strain was selected based on their primary screening against Gram positive Staphylococcus aureus, Enterococcus faecalis and Gram negative Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi bacterial pathogens. The selected strain was identified as Streptomyces sp CMSTAAHL-4 by 16S rRNA sequencing. The media optimization for secondary metabolites production was performed by One-Variable at a Time and Response Surface Methodology-Central Composite Design. Minimum inhibitory concentration and minimum bacterial concentration for the extracted secondary metabolites were determined. The antioxidant potential of the secondary metabolites showed that the concentration of the metabolites increases, with the percentage of inhibition. The anti-inflammatory activity of the secondary metabolites found that maximum activity was observed at 500 µg/ml of the metabolites. Alcohols, alkenes, alkynes, alkyl halides, carboxylic acids, aliphatic esters functional groups were identified by fourier transform infrared spectroscopy, gas chromatography and mass spectrometer analysis of the secondary metabolites revealed five bioactive compounds. The X-ray diffraction analysis revealed that the secondary metabolites are amorphous. The thermogravimetric analysis showed the thermal stability of secondary metabolites. Atomic force microscopy analysis revealed specific structural characteristics of the secondary metabolites, which may be associated with their potential biological activities. Conclusions The results showed that the antibacterial, antioxidant, and anti-inflammatory chemicals present in the isolated secondary metabolites give them therapeutic properties.https://doi.org/10.1186/s12866-025-03763-5AntimicrobialAntioxidantsMangrove Bio-prospectingStreptomyces sp. CMSTAAHI-4Secondary metabolites |
| spellingShingle | Selvaraj Jeraldin Nisha Ganapathi Uma Ramamoorthy Sathishkumar Vincent Samuel Gnana Prakash Rimal Isaac Thavasimuthu Citarasu Optimization and characterization of bioactive secondary metabolites from Streptomyces sp CMSTAAHL-4 isolated from mangrove sediment BMC Microbiology Antimicrobial Antioxidants Mangrove Bio-prospecting Streptomyces sp. CMSTAAHI-4 Secondary metabolites |
| title | Optimization and characterization of bioactive secondary metabolites from Streptomyces sp CMSTAAHL-4 isolated from mangrove sediment |
| title_full | Optimization and characterization of bioactive secondary metabolites from Streptomyces sp CMSTAAHL-4 isolated from mangrove sediment |
| title_fullStr | Optimization and characterization of bioactive secondary metabolites from Streptomyces sp CMSTAAHL-4 isolated from mangrove sediment |
| title_full_unstemmed | Optimization and characterization of bioactive secondary metabolites from Streptomyces sp CMSTAAHL-4 isolated from mangrove sediment |
| title_short | Optimization and characterization of bioactive secondary metabolites from Streptomyces sp CMSTAAHL-4 isolated from mangrove sediment |
| title_sort | optimization and characterization of bioactive secondary metabolites from streptomyces sp cmstaahl 4 isolated from mangrove sediment |
| topic | Antimicrobial Antioxidants Mangrove Bio-prospecting Streptomyces sp. CMSTAAHI-4 Secondary metabolites |
| url | https://doi.org/10.1186/s12866-025-03763-5 |
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