Investigating the Expression of c-Myc and BIRC5 in TRAIL and PBOX-15 treated Metastatic Prostate Cancer Cells
Purpose Metastatic prostate cancer is associated with poor survival rate and limited treatment options. Overexpression of c-Myc and BIRC5 has been reported in prostate cancer cells which contribute to the development of the tumour environment. The specific tumour receptor mechanism of the death liga...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Technological University Dublin
2024-12-01
|
| Series: | SURE Journal: (Science Undergraduate Research Experience Journal) |
| Subjects: | |
| Online Access: | https://arrow.tudublin.ie/sure_j/vol6/iss1/10 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832576229849432064 |
|---|---|
| author | Corinne Kai Leng Chew Hannah O'Toole Daniela M Zisterer Seema M. Nathwani Jade K. Pollock |
| author_facet | Corinne Kai Leng Chew Hannah O'Toole Daniela M Zisterer Seema M. Nathwani Jade K. Pollock |
| author_sort | Corinne Kai Leng Chew |
| collection | DOAJ |
| description | Purpose Metastatic prostate cancer is associated with poor survival rate and limited treatment options. Overexpression of c-Myc and BIRC5 has been reported in prostate cancer cells which contribute to the development of the tumour environment. The specific tumour receptor mechanism of the death ligand TRAIL (tumour necrosis factor related apoptosis inducing ligand) has attracted remarkable therapeutic interest. However, many cancer cells are resistant to TRAIL monotherapy. A novel microtubule-targeting agent, PBOX-15 is known to exhibit anti-tumour activity in cancer cell lines including those with multi-drug resistance. Previous studies demonstrated that PBOX-15 is capable in sensitising cancer cells to TRAIL-mediated cell death by upregulation of death receptors and downregulation of related oncogenes. This study examined the effect of TRAIL, PBOX-15 and the combination on the target genes in prostate cancer cells. Methods RNA was extracted from treated LNCaP cells. cDNA was synthesized, and gene specific primers were subsequently used for amplification in a polymerase chain reaction (PCR) assay. Gene expression was determined using gel electrophoresis and visualised using G:BOX. The intensity of DNA bands for each sample was analysed through gel densitometry. Results c-Myc and BIRC5 expression was detected in the LNCaP cell line following a 24-hour treatment time with each treatment. TRAIL increased expression of c-Myc and BIRC5. In contrast, c-Myc expression was downregulated by PBOX-15, and PBOX-15 in combination with TRAIL showed further decreased of both c-Myc and BIRC5 expression in the LNCaP cell line. Conclusions The presence of c-Myc and BIRC5 in LNCaP cell suggested the potential as therapeutic targets in metastatic prostate cancer. PBOX-15 presented an ability to sensitise cells to the effects of TRAIL, thus provide insight in potential application of the combination for metastatic prostate cancer. |
| format | Article |
| id | doaj-art-c9897cd6a4a441c6898f1dba572de869 |
| institution | Kabale University |
| issn | 2990-8167 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Technological University Dublin |
| record_format | Article |
| series | SURE Journal: (Science Undergraduate Research Experience Journal) |
| spelling | doaj-art-c9897cd6a4a441c6898f1dba572de8692025-01-31T10:28:14ZengTechnological University DublinSURE Journal: (Science Undergraduate Research Experience Journal)2990-81672024-12-016110.21427/jxan-jh87Investigating the Expression of c-Myc and BIRC5 in TRAIL and PBOX-15 treated Metastatic Prostate Cancer CellsCorinne Kai Leng Chew0Hannah O'Toole1Daniela M Zisterer2Seema M. Nathwani3Jade K. Pollock4Dundalk Institute of TechnologyUniversity College DublinTrinity College DublinUniversity College DublinDundalk Institute of TechnologyPurpose Metastatic prostate cancer is associated with poor survival rate and limited treatment options. Overexpression of c-Myc and BIRC5 has been reported in prostate cancer cells which contribute to the development of the tumour environment. The specific tumour receptor mechanism of the death ligand TRAIL (tumour necrosis factor related apoptosis inducing ligand) has attracted remarkable therapeutic interest. However, many cancer cells are resistant to TRAIL monotherapy. A novel microtubule-targeting agent, PBOX-15 is known to exhibit anti-tumour activity in cancer cell lines including those with multi-drug resistance. Previous studies demonstrated that PBOX-15 is capable in sensitising cancer cells to TRAIL-mediated cell death by upregulation of death receptors and downregulation of related oncogenes. This study examined the effect of TRAIL, PBOX-15 and the combination on the target genes in prostate cancer cells. Methods RNA was extracted from treated LNCaP cells. cDNA was synthesized, and gene specific primers were subsequently used for amplification in a polymerase chain reaction (PCR) assay. Gene expression was determined using gel electrophoresis and visualised using G:BOX. The intensity of DNA bands for each sample was analysed through gel densitometry. Results c-Myc and BIRC5 expression was detected in the LNCaP cell line following a 24-hour treatment time with each treatment. TRAIL increased expression of c-Myc and BIRC5. In contrast, c-Myc expression was downregulated by PBOX-15, and PBOX-15 in combination with TRAIL showed further decreased of both c-Myc and BIRC5 expression in the LNCaP cell line. Conclusions The presence of c-Myc and BIRC5 in LNCaP cell suggested the potential as therapeutic targets in metastatic prostate cancer. PBOX-15 presented an ability to sensitise cells to the effects of TRAIL, thus provide insight in potential application of the combination for metastatic prostate cancer.https://arrow.tudublin.ie/sure_j/vol6/iss1/10metastatic prostate cancerc-mycbirc5pbox-15microtubule-targeting agent |
| spellingShingle | Corinne Kai Leng Chew Hannah O'Toole Daniela M Zisterer Seema M. Nathwani Jade K. Pollock Investigating the Expression of c-Myc and BIRC5 in TRAIL and PBOX-15 treated Metastatic Prostate Cancer Cells SURE Journal: (Science Undergraduate Research Experience Journal) metastatic prostate cancer c-myc birc5 pbox-15 microtubule-targeting agent |
| title | Investigating the Expression of c-Myc and BIRC5 in TRAIL and PBOX-15 treated Metastatic Prostate Cancer Cells |
| title_full | Investigating the Expression of c-Myc and BIRC5 in TRAIL and PBOX-15 treated Metastatic Prostate Cancer Cells |
| title_fullStr | Investigating the Expression of c-Myc and BIRC5 in TRAIL and PBOX-15 treated Metastatic Prostate Cancer Cells |
| title_full_unstemmed | Investigating the Expression of c-Myc and BIRC5 in TRAIL and PBOX-15 treated Metastatic Prostate Cancer Cells |
| title_short | Investigating the Expression of c-Myc and BIRC5 in TRAIL and PBOX-15 treated Metastatic Prostate Cancer Cells |
| title_sort | investigating the expression of c myc and birc5 in trail and pbox 15 treated metastatic prostate cancer cells |
| topic | metastatic prostate cancer c-myc birc5 pbox-15 microtubule-targeting agent |
| url | https://arrow.tudublin.ie/sure_j/vol6/iss1/10 |
| work_keys_str_mv | AT corinnekailengchew investigatingtheexpressionofcmycandbirc5intrailandpbox15treatedmetastaticprostatecancercells AT hannahotoole investigatingtheexpressionofcmycandbirc5intrailandpbox15treatedmetastaticprostatecancercells AT danielamzisterer investigatingtheexpressionofcmycandbirc5intrailandpbox15treatedmetastaticprostatecancercells AT seemamnathwani investigatingtheexpressionofcmycandbirc5intrailandpbox15treatedmetastaticprostatecancercells AT jadekpollock investigatingtheexpressionofcmycandbirc5intrailandpbox15treatedmetastaticprostatecancercells |