HEMAGGLUTINATION AND ANTIGENIC PROPERTIES OF AVIBACTERIUM PARAGALLINARUM ISOLATES RECOVERED IN THE RUSSIAN FEDERATION AND REPUBLIC OF BELARUS
The paper demonstrates examination results of antigenic properties of Avibacterium paragallinarum reference strain and ten isolates thereof recovered in the Russian Federation and Republic of Belarus. Nine isolates and the reference strain demonstrated type L hemagglutinin (thermoliable, trypsin-sen...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Da Vinci Media
2019-01-01
|
| Series: | Ветеринария сегодня |
| Subjects: | |
| Online Access: | https://veterinary.arriah.ru/jour/article/view/389 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832086759451656192 |
|---|---|
| author | A. V. Potehin V. A. Yevgrafova D. B. Andreychuk |
| author_facet | A. V. Potehin V. A. Yevgrafova D. B. Andreychuk |
| author_sort | A. V. Potehin |
| collection | DOAJ |
| description | The paper demonstrates examination results of antigenic properties of Avibacterium paragallinarum reference strain and ten isolates thereof recovered in the Russian Federation and Republic of Belarus. Nine isolates and the reference strain demonstrated type L hemagglutinin (thermoliable, trypsin-sensitive, active against fresh and glutaraldehyde-treated RBC). One isolate was marked by type Hl hemagglutinin (thermoliable, trypsin-resistant, active only against glutaraldehydetreated RBC). The reference strain antigen proved to be inagglutinable by homologous antiserum, and unable to agglutinate RBCs due to hyaluronic acid in the capsular substance. Serological and hemagglutination non-reactivity was removed through the cell treatment with hyaluronidase. The agglutination test demonstrated that seven out of ten tested isolates belonged to the same serological group; herewith, the proportion of the bilateral antigenic relatedness amounted to ≥78.4%. HI results demonstrated ≥92.6% antigenic relatedness of the tested isolates being indicative of the fact that they belonged not only to the same serological group but also to the same serotype. No serological relatedness was identifed between the tested isolates and reference strain of serogroup A both using agglutination test (≤23.6%) and HI (≤12.2%). Polymerase chain reaction demonstrated that all the isolates recovered in the Russian Federation and Republic of Belarus in 2014-2016 belonged to serogroup B. |
| format | Article |
| id | doaj-art-c983aa7893784cfaaa9083872e6ccc62 |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-c983aa7893784cfaaa9083872e6ccc622025-02-06T09:52:07ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592019-01-0104313810.29326/2304-196X-2018-4-27-31-38382HEMAGGLUTINATION AND ANTIGENIC PROPERTIES OF AVIBACTERIUM PARAGALLINARUM ISOLATES RECOVERED IN THE RUSSIAN FEDERATION AND REPUBLIC OF BELARUSA. V. Potehin0V. A. Yevgrafova1D. B. Andreychuk2FGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirThe paper demonstrates examination results of antigenic properties of Avibacterium paragallinarum reference strain and ten isolates thereof recovered in the Russian Federation and Republic of Belarus. Nine isolates and the reference strain demonstrated type L hemagglutinin (thermoliable, trypsin-sensitive, active against fresh and glutaraldehyde-treated RBC). One isolate was marked by type Hl hemagglutinin (thermoliable, trypsin-resistant, active only against glutaraldehydetreated RBC). The reference strain antigen proved to be inagglutinable by homologous antiserum, and unable to agglutinate RBCs due to hyaluronic acid in the capsular substance. Serological and hemagglutination non-reactivity was removed through the cell treatment with hyaluronidase. The agglutination test demonstrated that seven out of ten tested isolates belonged to the same serological group; herewith, the proportion of the bilateral antigenic relatedness amounted to ≥78.4%. HI results demonstrated ≥92.6% antigenic relatedness of the tested isolates being indicative of the fact that they belonged not only to the same serological group but also to the same serotype. No serological relatedness was identifed between the tested isolates and reference strain of serogroup A both using agglutination test (≤23.6%) and HI (≤12.2%). Polymerase chain reaction demonstrated that all the isolates recovered in the Russian Federation and Republic of Belarus in 2014-2016 belonged to serogroup B.https://veterinary.arriah.ru/jour/article/view/389chicken infectious coryza (haemophilus infection)isolatesagglutininshemagglutininsantigenic relatednessavibacterium paragallinarum |
| spellingShingle | A. V. Potehin V. A. Yevgrafova D. B. Andreychuk HEMAGGLUTINATION AND ANTIGENIC PROPERTIES OF AVIBACTERIUM PARAGALLINARUM ISOLATES RECOVERED IN THE RUSSIAN FEDERATION AND REPUBLIC OF BELARUS Ветеринария сегодня chicken infectious coryza (haemophilus infection) isolates agglutinins hemagglutinins antigenic relatedness avibacterium paragallinarum |
| title | HEMAGGLUTINATION AND ANTIGENIC PROPERTIES OF AVIBACTERIUM PARAGALLINARUM ISOLATES RECOVERED IN THE RUSSIAN FEDERATION AND REPUBLIC OF BELARUS |
| title_full | HEMAGGLUTINATION AND ANTIGENIC PROPERTIES OF AVIBACTERIUM PARAGALLINARUM ISOLATES RECOVERED IN THE RUSSIAN FEDERATION AND REPUBLIC OF BELARUS |
| title_fullStr | HEMAGGLUTINATION AND ANTIGENIC PROPERTIES OF AVIBACTERIUM PARAGALLINARUM ISOLATES RECOVERED IN THE RUSSIAN FEDERATION AND REPUBLIC OF BELARUS |
| title_full_unstemmed | HEMAGGLUTINATION AND ANTIGENIC PROPERTIES OF AVIBACTERIUM PARAGALLINARUM ISOLATES RECOVERED IN THE RUSSIAN FEDERATION AND REPUBLIC OF BELARUS |
| title_short | HEMAGGLUTINATION AND ANTIGENIC PROPERTIES OF AVIBACTERIUM PARAGALLINARUM ISOLATES RECOVERED IN THE RUSSIAN FEDERATION AND REPUBLIC OF BELARUS |
| title_sort | hemagglutination and antigenic properties of avibacterium paragallinarum isolates recovered in the russian federation and republic of belarus |
| topic | chicken infectious coryza (haemophilus infection) isolates agglutinins hemagglutinins antigenic relatedness avibacterium paragallinarum |
| url | https://veterinary.arriah.ru/jour/article/view/389 |
| work_keys_str_mv | AT avpotehin hemagglutinationandantigenicpropertiesofavibacteriumparagallinarumisolatesrecoveredintherussianfederationandrepublicofbelarus AT vayevgrafova hemagglutinationandantigenicpropertiesofavibacteriumparagallinarumisolatesrecoveredintherussianfederationandrepublicofbelarus AT dbandreychuk hemagglutinationandantigenicpropertiesofavibacteriumparagallinarumisolatesrecoveredintherussianfederationandrepublicofbelarus |