Rapid Evaluation of Mutant Exon-11 in c-kit in a Recurrent MCT Case Using CD117 Immunocytofluorescence, FACS-Cell Sorting, and PCR
A 13-year-old, poodle-mixed, male dog was referred to the oncology unit in our faculty’s small animal teaching hospital with the problem of rapid recurrent MCT. The owner and the veterinarian would like to use a tyrosine kinase inhibitor (TKI) for the dog. Therefore, fine-needle aspiration (FNA) was...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Case Reports in Veterinary Medicine |
| Online Access: | http://dx.doi.org/10.1155/2013/728167 |
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| author | Dettachai Ketpun Achariya Sailasuta Prapruddee Piyaviriyakul Nattawat Onlamoon Kovit Pattanapanyasat |
| author_facet | Dettachai Ketpun Achariya Sailasuta Prapruddee Piyaviriyakul Nattawat Onlamoon Kovit Pattanapanyasat |
| author_sort | Dettachai Ketpun |
| collection | DOAJ |
| description | A 13-year-old, poodle-mixed, male dog was referred to the oncology unit in our faculty’s small animal teaching hospital with the problem of rapid recurrent MCT. The owner and the veterinarian would like to use a tyrosine kinase inhibitor (TKI) for the dog. Therefore, fine-needle aspiration (FNA) was performed to collect the MCT cells and these cells were submitted to our laboratory for the detection of internal-tandem-duplicated (ITD) mutation of exon-11 in c-kit, prior to the treatment. The aim of this paper is to demonstrate the use of combinatorial protocol for the rapid evaluation of ITD mutation in MCT cells harvested by FNA. However, there was no ITD-mutant exon-11 that had been observed in this case. |
| format | Article |
| id | doaj-art-c94e4eb783f84390ad83ce68750f6f86 |
| institution | Kabale University |
| issn | 2090-7001 2090-701X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Case Reports in Veterinary Medicine |
| spelling | doaj-art-c94e4eb783f84390ad83ce68750f6f862025-02-03T01:03:31ZengWileyCase Reports in Veterinary Medicine2090-70012090-701X2013-01-01201310.1155/2013/728167728167Rapid Evaluation of Mutant Exon-11 in c-kit in a Recurrent MCT Case Using CD117 Immunocytofluorescence, FACS-Cell Sorting, and PCRDettachai Ketpun0Achariya Sailasuta1Prapruddee Piyaviriyakul2Nattawat Onlamoon3Kovit Pattanapanyasat4STAR, Molecular Biology Research on Animal Oncology, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandSTAR, Molecular Biology Research on Animal Oncology, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandBiochemistry Unit, Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandOffice for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10770, ThailandOffice for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10770, ThailandA 13-year-old, poodle-mixed, male dog was referred to the oncology unit in our faculty’s small animal teaching hospital with the problem of rapid recurrent MCT. The owner and the veterinarian would like to use a tyrosine kinase inhibitor (TKI) for the dog. Therefore, fine-needle aspiration (FNA) was performed to collect the MCT cells and these cells were submitted to our laboratory for the detection of internal-tandem-duplicated (ITD) mutation of exon-11 in c-kit, prior to the treatment. The aim of this paper is to demonstrate the use of combinatorial protocol for the rapid evaluation of ITD mutation in MCT cells harvested by FNA. However, there was no ITD-mutant exon-11 that had been observed in this case.http://dx.doi.org/10.1155/2013/728167 |
| spellingShingle | Dettachai Ketpun Achariya Sailasuta Prapruddee Piyaviriyakul Nattawat Onlamoon Kovit Pattanapanyasat Rapid Evaluation of Mutant Exon-11 in c-kit in a Recurrent MCT Case Using CD117 Immunocytofluorescence, FACS-Cell Sorting, and PCR Case Reports in Veterinary Medicine |
| title | Rapid Evaluation of Mutant Exon-11 in c-kit in a Recurrent MCT Case Using CD117 Immunocytofluorescence, FACS-Cell Sorting, and PCR |
| title_full | Rapid Evaluation of Mutant Exon-11 in c-kit in a Recurrent MCT Case Using CD117 Immunocytofluorescence, FACS-Cell Sorting, and PCR |
| title_fullStr | Rapid Evaluation of Mutant Exon-11 in c-kit in a Recurrent MCT Case Using CD117 Immunocytofluorescence, FACS-Cell Sorting, and PCR |
| title_full_unstemmed | Rapid Evaluation of Mutant Exon-11 in c-kit in a Recurrent MCT Case Using CD117 Immunocytofluorescence, FACS-Cell Sorting, and PCR |
| title_short | Rapid Evaluation of Mutant Exon-11 in c-kit in a Recurrent MCT Case Using CD117 Immunocytofluorescence, FACS-Cell Sorting, and PCR |
| title_sort | rapid evaluation of mutant exon 11 in c kit in a recurrent mct case using cd117 immunocytofluorescence facs cell sorting and pcr |
| url | http://dx.doi.org/10.1155/2013/728167 |
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