Inhibitory Effect and Mechanism of Hexanal on the Maturation of Peach-Shaped <i>Phallus impudicus</i>
<i>Phallus impudicus</i> is a fungus used as a medicine and nutrient-rich food. However, the shelf life of mature <i>Phallus impudicus</i> is only a few hours. Therefore, research on its preservation technology is essential for improving its economic value. This study investi...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/11/2/127 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <i>Phallus impudicus</i> is a fungus used as a medicine and nutrient-rich food. However, the shelf life of mature <i>Phallus impudicus</i> is only a few hours. Therefore, research on its preservation technology is essential for improving its economic value. This study investigated the effects of hexanal concentrations (25–100 μL/L) and treatment time (4–8 h) on the inhibition of peach-shaped <i>Phallus impudicus</i> (CK) maturation and found that the maturation rate was 25% under optimal conditions of 25 μL/L hexanal treatment for 6 h. Quantitative transcriptomic and lipidomic analyses were conducted among CK, mature <i>Phallus impudicus</i> (M-P), and hexanal-treated peach-shaped <i>Phallus impudicus</i> (H-P-P). In total, 2933 and 2746 differentially expressed genes (DEGs) and 156 and 111 differentially abundant lipids (DALs) were identified in CK vs. H-P-P and M-P vs. H-P-P, respectively. Functional analysis demonstrated that hexanal treatment inhibited phospholipase D gene expression and reduced phosphatidic acid abundance, thereby inhibiting the activation of the phosphatidylinositol signaling system and the signal amplification of the cell wall integrity mitogen-activated protein kinase pathway. These blocked signal transductions inhibited the gene expression of most β-glucanases, chitinases and chitin synthases, further affecting cell wall reconstruction. Moreover, hexanal treatment enhanced membrane stability by reducing the monogalactosyl diglyceride/digalactosyl diacylglycerol ratio and increasing the phosphatidylcholine/phosphatidylethanolamine ratio. This study contributed to the development of hexanal treatment as a postharvest preservation technology for <i>Phallus impudicus</i>. |
|---|---|
| ISSN: | 2309-608X |