Interleukin-5 Potentiates Sulfidopeptide Leukotriene Production by Human Eosinophils
Interleukin-5 (IL-5) has been shown to be a selective eosinophil growth and differentiation factor. In the present study, the effect of recombinant human IL-5 on human eosinophil sulfidopeptide leukotriene production was investigated. IL-5 did not affect leukotriene synthesis in unstimulated eosinop...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
1994-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935194000098 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832549264535846912 |
|---|---|
| author | A. J. M. Van Oosterhout A. Van Der Poel L. Koenderman D. Roos F. P. Nijkamp |
| author_facet | A. J. M. Van Oosterhout A. Van Der Poel L. Koenderman D. Roos F. P. Nijkamp |
| author_sort | A. J. M. Van Oosterhout |
| collection | DOAJ |
| description | Interleukin-5 (IL-5) has been shown to be a selective eosinophil
growth and differentiation factor. In the present study, the effect
of recombinant human IL-5 on human eosinophil sulfidopeptide
leukotriene production was investigated. IL-5 did not affect
leukotriene synthesis in unstimulated eosinophils. However, IL-5
potentiated leukotriene synthesis by eosinophils stimulated with
serum treated zymosan (STZ) or the calcium ionophore A23187 by
69% and 135%, respectively. The priming effect of IL-5
was dose dependent, with significant stimulation occurring at 1 000
U/ml for STZ and 100-1 000 U/ml for A23187. Pre-incubation with IL-5
did not increase leukotriene synthesis further. |
| format | Article |
| id | doaj-art-c8bb060f88f448d29e8c5a0f39d38a02 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1994-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-c8bb060f88f448d29e8c5a0f39d38a022025-02-03T06:11:46ZengWileyMediators of Inflammation0962-93511466-18611994-01-0131535510.1155/S0962935194000098Interleukin-5 Potentiates Sulfidopeptide Leukotriene Production by Human EosinophilsA. J. M. Van Oosterhout0A. Van Der Poel1L. Koenderman2D. Roos3F. P. Nijkamp4Department of Pharmacology, Faculty of Pharmacy, Utrecht University, P.O. Box 80.082, Utrecht 3508 TB, The NetherlandsDepartment of Pharmacology, Faculty of Pharmacy, Utrecht University, P.O. Box 80.082, Utrecht 3508 TB, The NetherlandsCentral Laboratory of the Netherlands Red Cross, Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology, University of Amsterdam, The NetherlandsCentral Laboratory of the Netherlands Red Cross, Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology, University of Amsterdam, The NetherlandsDepartment of Pharmacology, Faculty of Pharmacy, Utrecht University, P.O. Box 80.082, Utrecht 3508 TB, The NetherlandsInterleukin-5 (IL-5) has been shown to be a selective eosinophil growth and differentiation factor. In the present study, the effect of recombinant human IL-5 on human eosinophil sulfidopeptide leukotriene production was investigated. IL-5 did not affect leukotriene synthesis in unstimulated eosinophils. However, IL-5 potentiated leukotriene synthesis by eosinophils stimulated with serum treated zymosan (STZ) or the calcium ionophore A23187 by 69% and 135%, respectively. The priming effect of IL-5 was dose dependent, with significant stimulation occurring at 1 000 U/ml for STZ and 100-1 000 U/ml for A23187. Pre-incubation with IL-5 did not increase leukotriene synthesis further.http://dx.doi.org/10.1155/S0962935194000098 |
| spellingShingle | A. J. M. Van Oosterhout A. Van Der Poel L. Koenderman D. Roos F. P. Nijkamp Interleukin-5 Potentiates Sulfidopeptide Leukotriene Production by Human Eosinophils Mediators of Inflammation |
| title | Interleukin-5 Potentiates Sulfidopeptide Leukotriene Production by Human Eosinophils |
| title_full | Interleukin-5 Potentiates Sulfidopeptide Leukotriene Production by Human Eosinophils |
| title_fullStr | Interleukin-5 Potentiates Sulfidopeptide Leukotriene Production by Human Eosinophils |
| title_full_unstemmed | Interleukin-5 Potentiates Sulfidopeptide Leukotriene Production by Human Eosinophils |
| title_short | Interleukin-5 Potentiates Sulfidopeptide Leukotriene Production by Human Eosinophils |
| title_sort | interleukin 5 potentiates sulfidopeptide leukotriene production by human eosinophils |
| url | http://dx.doi.org/10.1155/S0962935194000098 |
| work_keys_str_mv | AT ajmvanoosterhout interleukin5potentiatessulfidopeptideleukotrieneproductionbyhumaneosinophils AT avanderpoel interleukin5potentiatessulfidopeptideleukotrieneproductionbyhumaneosinophils AT lkoenderman interleukin5potentiatessulfidopeptideleukotrieneproductionbyhumaneosinophils AT droos interleukin5potentiatessulfidopeptideleukotrieneproductionbyhumaneosinophils AT fpnijkamp interleukin5potentiatessulfidopeptideleukotrieneproductionbyhumaneosinophils |