The Effect of Selenium Against Cadmium-Induced Nephrotoxicity in Rats: The Role of the TRPM2 Channel
This study investigated the protective effect of selenium (Se) in a cadmium (Cd)-induced nephrotoxicity model in rats and the role of the TRPM2 channel in this mechanism. For this purpose, Cd (25 mg/kg orally), Se (0.5 mg/kg i.p.), and 2-aminoethoxydiphenyl borate (2-APB), a TRPM2 channel antagonist...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Toxics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2305-6304/13/2/87 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850231383556882432 |
|---|---|
| author | Ömer Faruk Keleş Mehmet Hafit Bayir Hacı Ahmet Çiçek Adem Ahlatcı Kenan Yıldızhan |
| author_facet | Ömer Faruk Keleş Mehmet Hafit Bayir Hacı Ahmet Çiçek Adem Ahlatcı Kenan Yıldızhan |
| author_sort | Ömer Faruk Keleş |
| collection | DOAJ |
| description | This study investigated the protective effect of selenium (Se) in a cadmium (Cd)-induced nephrotoxicity model in rats and the role of the TRPM2 channel in this mechanism. For this purpose, Cd (25 mg/kg orally), Se (0.5 mg/kg i.p.), and 2-aminoethoxydiphenyl borate (2-APB), a TRPM2 channel antagonist, (3 mg/kg i.p.) were administered to rats every day for 5 days. At the end of the study, kidney tissues were analysed using histological and biochemical methods. A histopathological examination revealed congestion, tubular degeneration, necrosis, and glomerular adhesion in the Cd group. However, these lesions were significantly reduced in the Cd + Se and Cd + 2-APB groups, while the Cd + Se + 2-APB group showed a histological appearance similar to the control group. Immunohistochemical analysis revealed that Caspase-3, Bax, and TRPM2 expression was higher in the Cd group, while these levels were lower in the Se and 2-APB treatment groups (<i>p</i> < 0.05). Among the groups that received Cd, urea, creatinine, TOS, TNF-α, and IL-1β levels were at the highest level in the Cd group, while TAS level was at the lowest level (<i>p</i> < 0.05). The Se and 2-APB treatment modulated these parameters; however, Se + 2-APB treatment reduced urea, creatinine, TOS, TNF-α, and IL-1β levels to the lowest level compared to the Cd groups and brought the TAS level closer to the control group (<i>p</i> < 0.05). These findings indicated that targeting TRPM2 channel inactivation together with the selenium treatment could alleviate Cd-induced nephrotoxicity. |
| format | Article |
| id | doaj-art-c8a7bf18cf1c49ad9d83d19e61d8d8e3 |
| institution | OA Journals |
| issn | 2305-6304 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj-art-c8a7bf18cf1c49ad9d83d19e61d8d8e32025-08-20T02:03:32ZengMDPI AGToxics2305-63042025-01-011328710.3390/toxics13020087The Effect of Selenium Against Cadmium-Induced Nephrotoxicity in Rats: The Role of the TRPM2 ChannelÖmer Faruk Keleş0Mehmet Hafit Bayir1Hacı Ahmet Çiçek2Adem Ahlatcı3Kenan Yıldızhan4Department of Pathology, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, 65080 Van, TürkiyeDepartment of Histology, Faculty of Medicine, Van Yuzuncu Yil University, 65080 Van, TürkiyeDepartment of Pathology, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, 65080 Van, TürkiyeVocational School of Health Services, Van Yuzuncu Yil University, 65080 Van, TürkiyeDepartment of Biophysics, Faculty of Medicine, Van Yuzuncu Yil University, 65080 Van, TürkiyeThis study investigated the protective effect of selenium (Se) in a cadmium (Cd)-induced nephrotoxicity model in rats and the role of the TRPM2 channel in this mechanism. For this purpose, Cd (25 mg/kg orally), Se (0.5 mg/kg i.p.), and 2-aminoethoxydiphenyl borate (2-APB), a TRPM2 channel antagonist, (3 mg/kg i.p.) were administered to rats every day for 5 days. At the end of the study, kidney tissues were analysed using histological and biochemical methods. A histopathological examination revealed congestion, tubular degeneration, necrosis, and glomerular adhesion in the Cd group. However, these lesions were significantly reduced in the Cd + Se and Cd + 2-APB groups, while the Cd + Se + 2-APB group showed a histological appearance similar to the control group. Immunohistochemical analysis revealed that Caspase-3, Bax, and TRPM2 expression was higher in the Cd group, while these levels were lower in the Se and 2-APB treatment groups (<i>p</i> < 0.05). Among the groups that received Cd, urea, creatinine, TOS, TNF-α, and IL-1β levels were at the highest level in the Cd group, while TAS level was at the lowest level (<i>p</i> < 0.05). The Se and 2-APB treatment modulated these parameters; however, Se + 2-APB treatment reduced urea, creatinine, TOS, TNF-α, and IL-1β levels to the lowest level compared to the Cd groups and brought the TAS level closer to the control group (<i>p</i> < 0.05). These findings indicated that targeting TRPM2 channel inactivation together with the selenium treatment could alleviate Cd-induced nephrotoxicity.https://www.mdpi.com/2305-6304/13/2/87cadmiumseleniumTRPM2 channelhistopathologyimmunohistochemicallyrat |
| spellingShingle | Ömer Faruk Keleş Mehmet Hafit Bayir Hacı Ahmet Çiçek Adem Ahlatcı Kenan Yıldızhan The Effect of Selenium Against Cadmium-Induced Nephrotoxicity in Rats: The Role of the TRPM2 Channel Toxics cadmium selenium TRPM2 channel histopathology immunohistochemically rat |
| title | The Effect of Selenium Against Cadmium-Induced Nephrotoxicity in Rats: The Role of the TRPM2 Channel |
| title_full | The Effect of Selenium Against Cadmium-Induced Nephrotoxicity in Rats: The Role of the TRPM2 Channel |
| title_fullStr | The Effect of Selenium Against Cadmium-Induced Nephrotoxicity in Rats: The Role of the TRPM2 Channel |
| title_full_unstemmed | The Effect of Selenium Against Cadmium-Induced Nephrotoxicity in Rats: The Role of the TRPM2 Channel |
| title_short | The Effect of Selenium Against Cadmium-Induced Nephrotoxicity in Rats: The Role of the TRPM2 Channel |
| title_sort | effect of selenium against cadmium induced nephrotoxicity in rats the role of the trpm2 channel |
| topic | cadmium selenium TRPM2 channel histopathology immunohistochemically rat |
| url | https://www.mdpi.com/2305-6304/13/2/87 |
| work_keys_str_mv | AT omerfarukkeles theeffectofseleniumagainstcadmiuminducednephrotoxicityinratstheroleofthetrpm2channel AT mehmethafitbayir theeffectofseleniumagainstcadmiuminducednephrotoxicityinratstheroleofthetrpm2channel AT hacıahmetcicek theeffectofseleniumagainstcadmiuminducednephrotoxicityinratstheroleofthetrpm2channel AT ademahlatcı theeffectofseleniumagainstcadmiuminducednephrotoxicityinratstheroleofthetrpm2channel AT kenanyıldızhan theeffectofseleniumagainstcadmiuminducednephrotoxicityinratstheroleofthetrpm2channel AT omerfarukkeles effectofseleniumagainstcadmiuminducednephrotoxicityinratstheroleofthetrpm2channel AT mehmethafitbayir effectofseleniumagainstcadmiuminducednephrotoxicityinratstheroleofthetrpm2channel AT hacıahmetcicek effectofseleniumagainstcadmiuminducednephrotoxicityinratstheroleofthetrpm2channel AT ademahlatcı effectofseleniumagainstcadmiuminducednephrotoxicityinratstheroleofthetrpm2channel AT kenanyıldızhan effectofseleniumagainstcadmiuminducednephrotoxicityinratstheroleofthetrpm2channel |