Effect of Cow Urine on the Growth Characteristics of Fusarium lateritium, an Important Coffee Fungus in Zimbabwe
An in vitro assay was carried out to establish if cow urine at different concentrations (500 µL/mL, 300 µL/mL, 200 µL/mL, and 100 µL/mL) can be used to control the growth of F. lateritium, the fungal agent causing Fusarium bark disease in coffee. The growth characteristics selected were conidial ger...
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Format: | Article |
Language: | English |
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Wiley
2014-01-01
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Series: | International Journal of Agronomy |
Online Access: | http://dx.doi.org/10.1155/2014/986068 |
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author | Timothy Gotora Lawrence Masaka Marvelous Sungirai |
author_facet | Timothy Gotora Lawrence Masaka Marvelous Sungirai |
author_sort | Timothy Gotora |
collection | DOAJ |
description | An in vitro assay was carried out to establish if cow urine at different concentrations (500 µL/mL, 300 µL/mL, 200 µL/mL, and 100 µL/mL) can be used to control the growth of F. lateritium, the fungal agent causing Fusarium bark disease in coffee. The growth characteristics selected were conidial germination, germ tube length, mycelial growth rate, and sporulation. Copper oxychloride 50% W.P. was the standard, distilled water was the negative control, and undiluted cow urine was the positive control. The undiluted cow urine was most effective in inhibiting fungal growth with the rest of the cow urine concentrations showing dose dependent efficacy compared to the negative control (P < 0.01). Copper oxychloride had the highest efficacy of all treatments with the exception of the inhibition of mycelial growth where undiluted cow urine had higher efficacy and sporulation where efficacy was comparable to undiluted cow urine. There is potential for the use of cow urine as a means of controlling Fusarium bark disease with other advantages being availability, low cost, and limited environmental damage. |
format | Article |
id | doaj-art-c861d22f02834399bcbf6dacfe17cefe |
institution | Kabale University |
issn | 1687-8159 1687-8167 |
language | English |
publishDate | 2014-01-01 |
publisher | Wiley |
record_format | Article |
series | International Journal of Agronomy |
spelling | doaj-art-c861d22f02834399bcbf6dacfe17cefe2025-02-03T05:52:12ZengWileyInternational Journal of Agronomy1687-81591687-81672014-01-01201410.1155/2014/986068986068Effect of Cow Urine on the Growth Characteristics of Fusarium lateritium, an Important Coffee Fungus in ZimbabweTimothy Gotora0Lawrence Masaka1Marvelous Sungirai2Department of Applied Biosciences and Biotechnology, Midlands State University, Private Bag 9055, Gweru, ZimbabweDepartment of Livestock and Wildlife Management, Midlands State University, Private Bag 9055, Gweru, ZimbabweDepartment of Livestock and Wildlife Management, Midlands State University, Private Bag 9055, Gweru, ZimbabweAn in vitro assay was carried out to establish if cow urine at different concentrations (500 µL/mL, 300 µL/mL, 200 µL/mL, and 100 µL/mL) can be used to control the growth of F. lateritium, the fungal agent causing Fusarium bark disease in coffee. The growth characteristics selected were conidial germination, germ tube length, mycelial growth rate, and sporulation. Copper oxychloride 50% W.P. was the standard, distilled water was the negative control, and undiluted cow urine was the positive control. The undiluted cow urine was most effective in inhibiting fungal growth with the rest of the cow urine concentrations showing dose dependent efficacy compared to the negative control (P < 0.01). Copper oxychloride had the highest efficacy of all treatments with the exception of the inhibition of mycelial growth where undiluted cow urine had higher efficacy and sporulation where efficacy was comparable to undiluted cow urine. There is potential for the use of cow urine as a means of controlling Fusarium bark disease with other advantages being availability, low cost, and limited environmental damage.http://dx.doi.org/10.1155/2014/986068 |
spellingShingle | Timothy Gotora Lawrence Masaka Marvelous Sungirai Effect of Cow Urine on the Growth Characteristics of Fusarium lateritium, an Important Coffee Fungus in Zimbabwe International Journal of Agronomy |
title | Effect of Cow Urine on the Growth Characteristics of Fusarium lateritium, an Important Coffee Fungus in Zimbabwe |
title_full | Effect of Cow Urine on the Growth Characteristics of Fusarium lateritium, an Important Coffee Fungus in Zimbabwe |
title_fullStr | Effect of Cow Urine on the Growth Characteristics of Fusarium lateritium, an Important Coffee Fungus in Zimbabwe |
title_full_unstemmed | Effect of Cow Urine on the Growth Characteristics of Fusarium lateritium, an Important Coffee Fungus in Zimbabwe |
title_short | Effect of Cow Urine on the Growth Characteristics of Fusarium lateritium, an Important Coffee Fungus in Zimbabwe |
title_sort | effect of cow urine on the growth characteristics of fusarium lateritium an important coffee fungus in zimbabwe |
url | http://dx.doi.org/10.1155/2014/986068 |
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