Association of Corticosteroid Inhaler Type with Saliva Microbiome in Moderate-to-Severe Pediatric Asthma
<b>Background/Objectives</b>: Metered-dose inhalers (MDIs) and dry powder inhalers (DPIs) are common inhaled corticosteroid (ICS) inhaler devices. The difference in formulation and administration technique of these devices may influence oral cavity microbiota composition. We aimed to com...
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2025-01-01
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| author | Amir Hossein Alizadeh Bahmani Mahmoud I. Abdel-Aziz Simone Hashimoto Corinna Bang Susanne Brandstetter Paula Corcuera-Elosegui Andre Franke Mario Gorenjak Susanne Harner Parastoo Kheiroddin Leyre López-Fernández Anne H. Neerincx Maria Pino-Yanes Uroš Potočnik Olaia Sardón-Prado Antoaneta A. Toncheva Christine Wolff Michael Kabesch Aletta D. Kraneveld Susanne J. H. Vijverberg Anke H. Maitland-van der Zee on behalf of the SysPharmPediA consortium |
| author_facet | Amir Hossein Alizadeh Bahmani Mahmoud I. Abdel-Aziz Simone Hashimoto Corinna Bang Susanne Brandstetter Paula Corcuera-Elosegui Andre Franke Mario Gorenjak Susanne Harner Parastoo Kheiroddin Leyre López-Fernández Anne H. Neerincx Maria Pino-Yanes Uroš Potočnik Olaia Sardón-Prado Antoaneta A. Toncheva Christine Wolff Michael Kabesch Aletta D. Kraneveld Susanne J. H. Vijverberg Anke H. Maitland-van der Zee on behalf of the SysPharmPediA consortium |
| author_sort | Amir Hossein Alizadeh Bahmani |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: Metered-dose inhalers (MDIs) and dry powder inhalers (DPIs) are common inhaled corticosteroid (ICS) inhaler devices. The difference in formulation and administration technique of these devices may influence oral cavity microbiota composition. We aimed to compare the saliva microbiome in children with moderate-to-severe asthma using ICS via MDIs versus DPIs. <b>Methods</b>: Saliva samples collected from 143 children (6–17 yrs) with moderate-to-severe asthma across four European countries (The Netherlands, Germany, Spain, and Slovenia) as part of the SysPharmPediA cohort were subjected to 16S rRNA sequencing. The microbiome was compared using global diversity (α and β) between two groups of participants based on inhaler devices (MDI (n = 77) and DPI (n = 65)), and differential abundance was compared using the Analysis of Compositions of Microbiomes with the Bias Correction (ANCOM-BC) method. <b>Results</b>: No significant difference was observed in α-diversity between the two groups. However, β-diversity analysis revealed significant differences between groups using both Bray–Curtis and weighted UniFrac methods (adjusted <i>p</i>-value = 0.015 and 0.044, respectively). Significant differential abundance between groups, with higher relative abundance in the MDI group compared to the DPI group, was detected at the family level [Carnobacteriaceae (adjusted <i>p</i> = 0.033)] and at the genus level [<i>Granulicatella</i> (adjusted <i>p</i> = 0.021) and <i>Aggregatibacter</i> (adjusted <i>p</i> = 0.011)]. <b>Conclusions</b>: Types of ICS devices are associated with different saliva microbiome compositions in moderate-to-severe pediatric asthma. The causal relation between inhaler types and changes in saliva microbiota composition needs to be further evaluated, as well as whether this leads to different potential adverse effects in terms of occurrence and level of severity. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Biomedicines |
| spelling | doaj-art-c83fa664204f4f21b6b95e30c52102632025-01-24T13:23:58ZengMDPI AGBiomedicines2227-90592025-01-011318910.3390/biomedicines13010089Association of Corticosteroid Inhaler Type with Saliva Microbiome in Moderate-to-Severe Pediatric AsthmaAmir Hossein Alizadeh Bahmani0Mahmoud I. Abdel-Aziz1Simone Hashimoto2Corinna Bang3Susanne Brandstetter4Paula Corcuera-Elosegui5Andre Franke6Mario Gorenjak7Susanne Harner8Parastoo Kheiroddin9Leyre López-Fernández10Anne H. Neerincx11Maria Pino-Yanes12Uroš Potočnik13Olaia Sardón-Prado14Antoaneta A. Toncheva15Christine Wolff16Michael Kabesch17Aletta D. Kraneveld18Susanne J. H. Vijverberg19Anke H. Maitland-van der Zee20on behalf of the SysPharmPediA consortiumDepartment of Pulmonary Medicine, Amsterdam UMC Location University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The NetherlandsDepartment of Pulmonary Medicine, Amsterdam UMC Location University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The NetherlandsDepartment of Pulmonary Medicine, Amsterdam UMC Location University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The NetherlandsInstitute of Clinical Molecular Biology, Christian-Albrechts-University of Kiel, D-24105 Kiel, GermanyUniversity Children’s Hospital Regensburg (KUNO), University of Regensburg, D-93049 Regensburg, GermanyDivision of Pediatric Respiratory Medicine, Hospital Universitario Donostia, 20014 San Sebastián, SpainInstitute of Clinical Molecular Biology, Christian-Albrechts-University of Kiel, D-24105 Kiel, GermanyCenter for Human Molecular Genetics and Pharmacogenomics, Faculty of Medicine, University of Maribor, 2000 Maribor, SloveniaDepartment of Pediatric Pneumology and Allergy, University Children’s Hospital Regensburg (KUNO), D-93049 Regensburg, GermanyDepartment of Pediatric Pneumology and Allergy, University Children’s Hospital Regensburg (KUNO), D-93049 Regensburg, GermanyDivision of Pediatric Respiratory Medicine, Hospital Universitario Donostia, 20014 San Sebastián, SpainDepartment of Pulmonary Medicine, Amsterdam UMC Location University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The NetherlandsGenomics and Health Group, Department of Biochemistry, Microbiology, Cell Biology and Genetics, Universidad de La Laguna (ULL), 38200 Santa Cruz de Tenerife, SpainCenter for Human Molecular Genetics and Pharmacogenomics, Faculty of Medicine, University of Maribor, 2000 Maribor, SloveniaDivision of Pediatric Respiratory Medicine, Hospital Universitario Donostia, 20014 San Sebastián, SpainDepartment of Pediatric Pneumology and Allergy, University Children’s Hospital Regensburg (KUNO), D-93049 Regensburg, GermanyDepartment of Pediatric Pneumology and Allergy, University Children’s Hospital Regensburg (KUNO), D-93049 Regensburg, GermanyDepartment of Pediatric Pneumology and Allergy, University Children’s Hospital Regensburg (KUNO), D-93049 Regensburg, GermanyDivision of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Universiteitsweg 99, 3584 CG Utrecht, The NetherlandsDepartment of Pulmonary Medicine, Amsterdam UMC Location University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The NetherlandsDepartment of Pulmonary Medicine, Amsterdam UMC Location University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands<b>Background/Objectives</b>: Metered-dose inhalers (MDIs) and dry powder inhalers (DPIs) are common inhaled corticosteroid (ICS) inhaler devices. The difference in formulation and administration technique of these devices may influence oral cavity microbiota composition. We aimed to compare the saliva microbiome in children with moderate-to-severe asthma using ICS via MDIs versus DPIs. <b>Methods</b>: Saliva samples collected from 143 children (6–17 yrs) with moderate-to-severe asthma across four European countries (The Netherlands, Germany, Spain, and Slovenia) as part of the SysPharmPediA cohort were subjected to 16S rRNA sequencing. The microbiome was compared using global diversity (α and β) between two groups of participants based on inhaler devices (MDI (n = 77) and DPI (n = 65)), and differential abundance was compared using the Analysis of Compositions of Microbiomes with the Bias Correction (ANCOM-BC) method. <b>Results</b>: No significant difference was observed in α-diversity between the two groups. However, β-diversity analysis revealed significant differences between groups using both Bray–Curtis and weighted UniFrac methods (adjusted <i>p</i>-value = 0.015 and 0.044, respectively). Significant differential abundance between groups, with higher relative abundance in the MDI group compared to the DPI group, was detected at the family level [Carnobacteriaceae (adjusted <i>p</i> = 0.033)] and at the genus level [<i>Granulicatella</i> (adjusted <i>p</i> = 0.021) and <i>Aggregatibacter</i> (adjusted <i>p</i> = 0.011)]. <b>Conclusions</b>: Types of ICS devices are associated with different saliva microbiome compositions in moderate-to-severe pediatric asthma. The causal relation between inhaler types and changes in saliva microbiota composition needs to be further evaluated, as well as whether this leads to different potential adverse effects in terms of occurrence and level of severity.https://www.mdpi.com/2227-9059/13/1/89asthmainhaled corticosteroidsmetered-dose inhalerdry powder inhalermicrobiomesaliva |
| spellingShingle | Amir Hossein Alizadeh Bahmani Mahmoud I. Abdel-Aziz Simone Hashimoto Corinna Bang Susanne Brandstetter Paula Corcuera-Elosegui Andre Franke Mario Gorenjak Susanne Harner Parastoo Kheiroddin Leyre López-Fernández Anne H. Neerincx Maria Pino-Yanes Uroš Potočnik Olaia Sardón-Prado Antoaneta A. Toncheva Christine Wolff Michael Kabesch Aletta D. Kraneveld Susanne J. H. Vijverberg Anke H. Maitland-van der Zee on behalf of the SysPharmPediA consortium Association of Corticosteroid Inhaler Type with Saliva Microbiome in Moderate-to-Severe Pediatric Asthma Biomedicines asthma inhaled corticosteroids metered-dose inhaler dry powder inhaler microbiome saliva |
| title | Association of Corticosteroid Inhaler Type with Saliva Microbiome in Moderate-to-Severe Pediatric Asthma |
| title_full | Association of Corticosteroid Inhaler Type with Saliva Microbiome in Moderate-to-Severe Pediatric Asthma |
| title_fullStr | Association of Corticosteroid Inhaler Type with Saliva Microbiome in Moderate-to-Severe Pediatric Asthma |
| title_full_unstemmed | Association of Corticosteroid Inhaler Type with Saliva Microbiome in Moderate-to-Severe Pediatric Asthma |
| title_short | Association of Corticosteroid Inhaler Type with Saliva Microbiome in Moderate-to-Severe Pediatric Asthma |
| title_sort | association of corticosteroid inhaler type with saliva microbiome in moderate to severe pediatric asthma |
| topic | asthma inhaled corticosteroids metered-dose inhaler dry powder inhaler microbiome saliva |
| url | https://www.mdpi.com/2227-9059/13/1/89 |
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