Dynamic Production of Soluble Extracellular Polysaccharides by Streptococcus mutans
Caries development in the presence of Streptococcus mutans is associated not only with the production of extracellular water-insoluble polymers but also is based on water-soluble polysaccharides. The aim of this study was the evaluation of a novel glucan-specific Lectin assay for monitoring water-so...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2011/435830 |
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| author | Eva-Maria Decker Ilka Dietrich Christian Klein Christiane von Ohle |
| author_facet | Eva-Maria Decker Ilka Dietrich Christian Klein Christiane von Ohle |
| author_sort | Eva-Maria Decker |
| collection | DOAJ |
| description | Caries development in the presence of Streptococcus mutans is associated not only with the production of extracellular water-insoluble polymers but also is based on water-soluble polysaccharides. The aim of this study was the evaluation of a novel glucan-specific Lectin assay for monitoring water-soluble EPS produced by S. mutans during several growth periods in different media. S. mutans cultures were grown for 24 h, 48 h, and 144 h in medium deficient of sucrose (A) and medium supplemented with 5% sucrose (B). Microtiter well plates were coated with cell-free supernatants followed by the addition of labeled Concanavalin-A and enzyme substrate. The substrate reactions were kinetically detected at 405 nm. The validation of the assay was performed using carbohydrates dextran, xanthan, and sucrose as reference. This new Concanavalin-A-based assay showed the highest sensitivity for dextran and revealed that the glucan production of S. mutans reached its maximum at 144 h in medium B according to bacterial maturation. |
| format | Article |
| id | doaj-art-c76d697d50814702bcdba5f5598b2940 |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-c76d697d50814702bcdba5f5598b29402025-02-03T01:31:13ZengWileyInternational Journal of Dentistry1687-87281687-87362011-01-01201110.1155/2011/435830435830Dynamic Production of Soluble Extracellular Polysaccharides by Streptococcus mutansEva-Maria Decker0Ilka Dietrich1Christian Klein2Christiane von Ohle3Department of Operative Dentistry and Periodontology, Center of Dentistry, Oral Medicine, and Maxillofacial Surgery, Eberhard-Karls University Tübingen, 72076 Tübingen, GermanyDepartment of Operative Dentistry and Periodontology, Center of Dentistry, Oral Medicine, and Maxillofacial Surgery, Eberhard-Karls University Tübingen, 72076 Tübingen, GermanyDepartment of Operative Dentistry and Periodontology, Center of Dentistry, Oral Medicine, and Maxillofacial Surgery, Eberhard-Karls University Tübingen, 72076 Tübingen, GermanyDepartment of Operative Dentistry and Periodontology, Center of Dentistry, Oral Medicine, and Maxillofacial Surgery, Eberhard-Karls University Tübingen, 72076 Tübingen, GermanyCaries development in the presence of Streptococcus mutans is associated not only with the production of extracellular water-insoluble polymers but also is based on water-soluble polysaccharides. The aim of this study was the evaluation of a novel glucan-specific Lectin assay for monitoring water-soluble EPS produced by S. mutans during several growth periods in different media. S. mutans cultures were grown for 24 h, 48 h, and 144 h in medium deficient of sucrose (A) and medium supplemented with 5% sucrose (B). Microtiter well plates were coated with cell-free supernatants followed by the addition of labeled Concanavalin-A and enzyme substrate. The substrate reactions were kinetically detected at 405 nm. The validation of the assay was performed using carbohydrates dextran, xanthan, and sucrose as reference. This new Concanavalin-A-based assay showed the highest sensitivity for dextran and revealed that the glucan production of S. mutans reached its maximum at 144 h in medium B according to bacterial maturation.http://dx.doi.org/10.1155/2011/435830 |
| spellingShingle | Eva-Maria Decker Ilka Dietrich Christian Klein Christiane von Ohle Dynamic Production of Soluble Extracellular Polysaccharides by Streptococcus mutans International Journal of Dentistry |
| title | Dynamic Production of Soluble Extracellular Polysaccharides by Streptococcus mutans |
| title_full | Dynamic Production of Soluble Extracellular Polysaccharides by Streptococcus mutans |
| title_fullStr | Dynamic Production of Soluble Extracellular Polysaccharides by Streptococcus mutans |
| title_full_unstemmed | Dynamic Production of Soluble Extracellular Polysaccharides by Streptococcus mutans |
| title_short | Dynamic Production of Soluble Extracellular Polysaccharides by Streptococcus mutans |
| title_sort | dynamic production of soluble extracellular polysaccharides by streptococcus mutans |
| url | http://dx.doi.org/10.1155/2011/435830 |
| work_keys_str_mv | AT evamariadecker dynamicproductionofsolubleextracellularpolysaccharidesbystreptococcusmutans AT ilkadietrich dynamicproductionofsolubleextracellularpolysaccharidesbystreptococcusmutans AT christianklein dynamicproductionofsolubleextracellularpolysaccharidesbystreptococcusmutans AT christianevonohle dynamicproductionofsolubleextracellularpolysaccharidesbystreptococcusmutans |