Human citrate synthase kinetic simulation to fit rapid, direct, and thiol probe coupled kinetic data

Human citrate synthase kinetic simulation to fit rapid, direct, and thiol probe coupled kinetic data

Human citrate synthase (hCS) was kinetically characterized through full progress curve kinetic modelling using kinetic simulation, global fitting of the direct AcCoA to CoA transition, and a coupled thiol probe reaction to better determine the kinetics with low substrate concentration. Our analysis...

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Bibliographic Details
Main Authors: Noah Shackelford, Zach Zavodny, Samantha Schindler, Nathan Fancher, Allen A. Thomas, Michael A. Moxley
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:Biochemistry and Biophysics Reports
Subjects:
Enzyme kinetics
Global fitting
Kinetic simulation
Thiol probe
Citrate synthase
Online Access:http://www.sciencedirect.com/science/article/pii/S2405580825000019
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