Antimicrobial Activity of Aspergillus sp. from the Amazon Biome: Isolation of Kojic Acid
The antimicrobial potential of Aspergillus sp., isolated from the Amazon biome, which is stored at the Amazon Fungi Collection-CFAM at ILMD/FIOCRUZ, was evaluated. The fungal culture was cultivated in yeast extract agar and sucrose (YES) for cold extraction of the biocompounds in ethyl acetate at 28...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2022/4010018 |
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| author | Josy Caldas Rodrigues Weison Lima da Silva David Ribeiro da Silva Carolina Rabelo Maia Clarice Virginia Santos Goiabeira Haile Dean Figueiredo Chagas Gigliola Mayara Ayres D’Elia Gleica Soyan Barbosa Alves Viviane Zahner Cecilia Veronica Nunez Ormezinda Celeste Cristo Fernandes |
| author_facet | Josy Caldas Rodrigues Weison Lima da Silva David Ribeiro da Silva Carolina Rabelo Maia Clarice Virginia Santos Goiabeira Haile Dean Figueiredo Chagas Gigliola Mayara Ayres D’Elia Gleica Soyan Barbosa Alves Viviane Zahner Cecilia Veronica Nunez Ormezinda Celeste Cristo Fernandes |
| author_sort | Josy Caldas Rodrigues |
| collection | DOAJ |
| description | The antimicrobial potential of Aspergillus sp., isolated from the Amazon biome, which is stored at the Amazon Fungi Collection-CFAM at ILMD/FIOCRUZ, was evaluated. The fungal culture was cultivated in yeast extract agar and sucrose (YES) for cold extraction of the biocompounds in ethyl acetate at 28 °C for 7 days in a BOD type incubator. The obtained extract was evaluated for its antimicrobial activity against Candida albicans and Gram-positive and negative bacteria by the “cup plate” method and the determination of the minimum inhibitory concentration (MIC) by the broth microdilution method. The extract was subjected to thin layer chromatography (TLC) and fractionated by open and semipreparative column chromatography. The fractions of interest had their chemical constituents elucidated by nuclear magnetic resonance and mass spectrometry. The elucidated molecule was evaluated for cytotoxicity against the human fibroblast strain (MRC5). The extract presented inhibitory activity against both Gram-positive and negative bacteria, with the range of inhibition halos from 5.3 to 14 mm in diameter and an MIC ranging from 500 to 15.6 μg/mL. Seventy-one fractions were collected and TLC analysis suggested the presence of substances with double bond groups: coumarins, flavonoids, phenolic, alkaloids, and terpenes. NMR and MS analyses demonstrated that the isolated molecule was kojic acid. The results of the cytotoxicity test showed that MRC5 cells presented viability at concentrations from 500 to 7.81 μg/mL. The kojic acid molecule of Aspergillus sp., with antibacterial activity and moderate toxicity at the concentrations tested, is a promising prototype of an alternative active principle of an antimicrobial drug. |
| format | Article |
| id | doaj-art-c6c5a02064a440c88b90e786db0840fc |
| institution | Kabale University |
| issn | 1687-9198 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-c6c5a02064a440c88b90e786db0840fc2025-02-03T06:05:24ZengWileyInternational Journal of Microbiology1687-91982022-01-01202210.1155/2022/4010018Antimicrobial Activity of Aspergillus sp. from the Amazon Biome: Isolation of Kojic AcidJosy Caldas Rodrigues0Weison Lima da Silva1David Ribeiro da Silva2Carolina Rabelo Maia3Clarice Virginia Santos Goiabeira4Haile Dean Figueiredo Chagas5Gigliola Mayara Ayres D’Elia6Gleica Soyan Barbosa Alves7Viviane Zahner8Cecilia Veronica Nunez9Ormezinda Celeste Cristo Fernandes10Leônidas and Maria Deane Institute-ILMD/Fiocruz AmazôniaNational Institute of Amazonian ResearchNational Institute of Amazonian ResearchLeônidas and Maria Deane Institute-ILMD/Fiocruz AmazôniaLeônidas and Maria Deane Institute-ILMD/Fiocruz AmazôniaLeônidas and Maria Deane Institute-ILMD/Fiocruz AmazôniaLeônidas and Maria Deane Institute-ILMD/Fiocruz AmazôniaLeônidas and Maria Deane Institute-ILMD/Fiocruz AmazôniaOswaldo Cruz Institute/IOC/FiocruzNational Institute of Amazonian ResearchLeônidas and Maria Deane Institute-ILMD/Fiocruz AmazôniaThe antimicrobial potential of Aspergillus sp., isolated from the Amazon biome, which is stored at the Amazon Fungi Collection-CFAM at ILMD/FIOCRUZ, was evaluated. The fungal culture was cultivated in yeast extract agar and sucrose (YES) for cold extraction of the biocompounds in ethyl acetate at 28 °C for 7 days in a BOD type incubator. The obtained extract was evaluated for its antimicrobial activity against Candida albicans and Gram-positive and negative bacteria by the “cup plate” method and the determination of the minimum inhibitory concentration (MIC) by the broth microdilution method. The extract was subjected to thin layer chromatography (TLC) and fractionated by open and semipreparative column chromatography. The fractions of interest had their chemical constituents elucidated by nuclear magnetic resonance and mass spectrometry. The elucidated molecule was evaluated for cytotoxicity against the human fibroblast strain (MRC5). The extract presented inhibitory activity against both Gram-positive and negative bacteria, with the range of inhibition halos from 5.3 to 14 mm in diameter and an MIC ranging from 500 to 15.6 μg/mL. Seventy-one fractions were collected and TLC analysis suggested the presence of substances with double bond groups: coumarins, flavonoids, phenolic, alkaloids, and terpenes. NMR and MS analyses demonstrated that the isolated molecule was kojic acid. The results of the cytotoxicity test showed that MRC5 cells presented viability at concentrations from 500 to 7.81 μg/mL. The kojic acid molecule of Aspergillus sp., with antibacterial activity and moderate toxicity at the concentrations tested, is a promising prototype of an alternative active principle of an antimicrobial drug.http://dx.doi.org/10.1155/2022/4010018 |
| spellingShingle | Josy Caldas Rodrigues Weison Lima da Silva David Ribeiro da Silva Carolina Rabelo Maia Clarice Virginia Santos Goiabeira Haile Dean Figueiredo Chagas Gigliola Mayara Ayres D’Elia Gleica Soyan Barbosa Alves Viviane Zahner Cecilia Veronica Nunez Ormezinda Celeste Cristo Fernandes Antimicrobial Activity of Aspergillus sp. from the Amazon Biome: Isolation of Kojic Acid International Journal of Microbiology |
| title | Antimicrobial Activity of Aspergillus sp. from the Amazon Biome: Isolation of Kojic Acid |
| title_full | Antimicrobial Activity of Aspergillus sp. from the Amazon Biome: Isolation of Kojic Acid |
| title_fullStr | Antimicrobial Activity of Aspergillus sp. from the Amazon Biome: Isolation of Kojic Acid |
| title_full_unstemmed | Antimicrobial Activity of Aspergillus sp. from the Amazon Biome: Isolation of Kojic Acid |
| title_short | Antimicrobial Activity of Aspergillus sp. from the Amazon Biome: Isolation of Kojic Acid |
| title_sort | antimicrobial activity of aspergillus sp from the amazon biome isolation of kojic acid |
| url | http://dx.doi.org/10.1155/2022/4010018 |
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