Assembly of ascovirus HvAV-3h long DNA fragment using the Transformation-Associated Recombination (TAR) approach in yeast cells

Abstract Background Synthetic biology is a young but rapidly growing field that allows for assembling long DNA fragments, including complete chromosomes. A key approach for long-DNA assembly is the Transformation Associated Recombination (TAR), which relies on efficient homologous recombination in y...

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Bibliographic Details
Main Authors: Heba A. H. Zaghloul, Zhengkun Xiao, Hengrui Hu, Guo-Hua Huang
Format: Article
Language:English
Published: BMC 2025-05-01
Series:BMC Biotechnology
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Online Access:https://doi.org/10.1186/s12896-025-00964-8
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