Serum assisted PD-L1 aptamer screening for improving its stability
Abstract Aptamers have shown potential for diagnosing clinical markers and targeted treatment of diseases. However, their limited stability and short half-life hinder their broader applications. Here, a real sample assisted capture-SELEX strategy is proposed to enhance the aptamer stability, using t...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-85813-6 |
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| author | Yu Zhou Haozhe Zhang Yujing Ding Changyuan Yu Hao Li |
| author_facet | Yu Zhou Haozhe Zhang Yujing Ding Changyuan Yu Hao Li |
| author_sort | Yu Zhou |
| collection | DOAJ |
| description | Abstract Aptamers have shown potential for diagnosing clinical markers and targeted treatment of diseases. However, their limited stability and short half-life hinder their broader applications. Here, a real sample assisted capture-SELEX strategy is proposed to enhance the aptamer stability, using the selection of specific aptamer towards PD-L1 as an example. Through this developed selection strategy, the aptamer Apt-S1 with higher binding affinity and specificity towards PD-L1 was obtained as compared to the aptamer Apt-A2 which was screened by the traditional capture-SELEX strategy. Moreover, Apt-S1 exhibited a greater PD-L1 binding associated conformational change than Apt-A2, indicating its suitability as a biorecognition element. These findings highlight the potential of Apt-S1 in clinical applications requiring robust and specific targeting of PD-L1. Significantly, Apt-S1 exhibited a lower degradation rate in 10% diluted serum or pure human serum, under the physiological temperature and pH value, compared to Apt-A2. This observation suggested that Apt-S1 possesses higher stability and is more resistant to damage caused by the serum environmental factors, highlighting the superior stability of Apt-S1 over Apt-A2. Furthermore, defatted and deproteinized serum were used to investigate the potential reasons for the improved stability of Apt-S1. The results hinted that the pre-adaptation to nucleases present in serum during the selection process might have contributed to its higher stability. With its improved stability, higher affinity and specificity, Apt-S1 holds great potential for applications in PD-L1 assisted cancer diagnosis and treatment. Meanwhile, the results obtained in this work provide further evidence of the advantages of the real capture-SELEX strategy in improving aptamer stability compared to the traditional strategy. |
| format | Article |
| id | doaj-art-c617fc0854824f408ab3f7cfdd5906b9 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Scientific Reports |
| spelling | doaj-art-c617fc0854824f408ab3f7cfdd5906b92025-01-19T12:19:20ZengNature PortfolioScientific Reports2045-23222025-01-011511810.1038/s41598-025-85813-6Serum assisted PD-L1 aptamer screening for improving its stabilityYu Zhou0Haozhe Zhang1Yujing Ding2Changyuan Yu3Hao Li4School of Public Health, Jining Medical UniversityCollege of Life Science and Technology, Beijing University of Chemical TechnologyCollege of Life Science and Technology, Beijing University of Chemical TechnologyCollege of Life Science and Technology, Beijing University of Chemical TechnologySchool of Public Health, Jining Medical UniversityAbstract Aptamers have shown potential for diagnosing clinical markers and targeted treatment of diseases. However, their limited stability and short half-life hinder their broader applications. Here, a real sample assisted capture-SELEX strategy is proposed to enhance the aptamer stability, using the selection of specific aptamer towards PD-L1 as an example. Through this developed selection strategy, the aptamer Apt-S1 with higher binding affinity and specificity towards PD-L1 was obtained as compared to the aptamer Apt-A2 which was screened by the traditional capture-SELEX strategy. Moreover, Apt-S1 exhibited a greater PD-L1 binding associated conformational change than Apt-A2, indicating its suitability as a biorecognition element. These findings highlight the potential of Apt-S1 in clinical applications requiring robust and specific targeting of PD-L1. Significantly, Apt-S1 exhibited a lower degradation rate in 10% diluted serum or pure human serum, under the physiological temperature and pH value, compared to Apt-A2. This observation suggested that Apt-S1 possesses higher stability and is more resistant to damage caused by the serum environmental factors, highlighting the superior stability of Apt-S1 over Apt-A2. Furthermore, defatted and deproteinized serum were used to investigate the potential reasons for the improved stability of Apt-S1. The results hinted that the pre-adaptation to nucleases present in serum during the selection process might have contributed to its higher stability. With its improved stability, higher affinity and specificity, Apt-S1 holds great potential for applications in PD-L1 assisted cancer diagnosis and treatment. Meanwhile, the results obtained in this work provide further evidence of the advantages of the real capture-SELEX strategy in improving aptamer stability compared to the traditional strategy.https://doi.org/10.1038/s41598-025-85813-6Real capture-SELEX strategyAptamer stabilityAptamer selectionPD-L1Serum |
| spellingShingle | Yu Zhou Haozhe Zhang Yujing Ding Changyuan Yu Hao Li Serum assisted PD-L1 aptamer screening for improving its stability Scientific Reports Real capture-SELEX strategy Aptamer stability Aptamer selection PD-L1 Serum |
| title | Serum assisted PD-L1 aptamer screening for improving its stability |
| title_full | Serum assisted PD-L1 aptamer screening for improving its stability |
| title_fullStr | Serum assisted PD-L1 aptamer screening for improving its stability |
| title_full_unstemmed | Serum assisted PD-L1 aptamer screening for improving its stability |
| title_short | Serum assisted PD-L1 aptamer screening for improving its stability |
| title_sort | serum assisted pd l1 aptamer screening for improving its stability |
| topic | Real capture-SELEX strategy Aptamer stability Aptamer selection PD-L1 Serum |
| url | https://doi.org/10.1038/s41598-025-85813-6 |
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