Comparative performance evaluation of bisulfite- and enzyme-based DNA conversion methods

Abstract Background Bisulfite conversion (BC) has been the gold standard in DNA methylation profiling for decades. During this chemical process, non-methylated cytosines are converted into uracils, while methylated cytosines remain intact. Despite its popularity, BC has major drawbacks when used for...

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Bibliographic Details
Main Authors: Roy B. Simons, Faidra Karkala, Marta M. Kukk, Hieab H. H. Adams, Manfred Kayser, Athina Vidaki
Format: Article
Language:English
Published: BMC 2025-04-01
Series:Clinical Epigenetics
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Online Access:https://doi.org/10.1186/s13148-025-01855-7
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