Endothelin-1 Stimulates Monocytes in vitro to Release Chemotactic Activity Identified as Interleukin-8 and Monocyte Chemotactic Protein-1
In the present study we examined whether endothelin-1 stimulation of human monocytes causes release of chemotactic factors. It was found that monocytes released neutrophil- and monocyte-chemotactic activity in a dose- and time-dependent manner in response to ET-1. ET-1 did not show any chemotactic a...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
1994-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935194000207 |
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| Summary: | In the present study we examined whether endothelin-1 stimulation of
human monocytes causes release of chemotactic factors. It was found
that monocytes released neutrophil- and monocyte-chemotactic
activity in a dose- and time-dependent manner in response to ET-1.
ET-1 did not show any chemotactic activity by itself. NCA was
detected in monocyte supernatants in response to ET-1
(0.01–100 nM) after 1, 4, 8 and 24 h stimulation. MCA was
detected only after 24 h stimulation with ET-1 (0.1–100 nM).
Preincubation of the monocyte cultures with the lipoxygenase
inhibitors nordihydroguaiaretic acid (10−4 M) or
diethylcarbamazine (10−9 M) completely abolished
the appearance of NCA and MCA. NCA was neutralized by >
75% using a polyclonal antibody against human interleuktn-8.
The ET-1 induced release of IL-8 was confirmed by IL-8 ELISA. A
monoclonal antibody against human monocyte chemotactic protein-1
neutralized MCA by > 80%. It is concluded that ET-1
stimulation of monocytes in vitro causes release of
neutrophil- and monocyte-chemotactic activity identified as IL-8 and
MCP-I respectively. An intact lipoxygenase pathway is crucial for
this effect of ET-1 to occur. |
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| ISSN: | 0962-9351 1466-1861 |