Physiological and Metabolomics Analyses Revealed That Overexpression of CBL-Interacting Protein Kinase 23 Accelerate Tuber Sprouting in Potato
The potato (<i>Solanum tuberosum</i> L.) plays an important role in ensuring global food security. Potato tubers store abundant nutrients and are also reproductive organs. The adjustment of tuber sprouting plays a vital role in timely sowing and improving tuber product quality. CBL-inter...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
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| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/11/4/342 |
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| Summary: | The potato (<i>Solanum tuberosum</i> L.) plays an important role in ensuring global food security. Potato tubers store abundant nutrients and are also reproductive organs. The adjustment of tuber sprouting plays a vital role in timely sowing and improving tuber product quality. CBL-interacting protein kinases (CIPKs) exert an important function in the entire life cycle of plants and in coping with stress. In our present study, we found that the <i>StCIPK23</i> expression level increased during storage and that overexpression of <i>StCIPK23</i> can accelerate tuber sprouting. Physiological assays indicated that overexpressing <i>StCIPK23</i> altered carbohydrate metabolism and antioxidant-related enzyme activities during storage. Starch branching enzyme (<i>SBEI</i>) gene expression was upregulated, while sucrose synthase (<i>SS</i>), 3-phosphoglyceric phosphokinase (<i>PGK</i>), and glyceraldehyde-3-phosphate dehydrogenase 1 (<i>GAPC1</i>) gene expression were downregulated in <i>StCIPK23</i>-overexpressing potato. High gibberellin (GA) content and low abscisic acid (ABA) content were also detected in transgenic tubers. We conducted metabolomics analysis on bud eyes, and the results showed a total of 94 differential metabolites were found. Among them, 61 metabolites were increased, the top three metabolites were coumaryl alcohol, glutathione and quercetin–glucoside–glucoside–rhamnoside. Our results suggest that StCIPK23 is a positive regulator of potato tuber sprouting. |
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| ISSN: | 2311-7524 |