Serum Cytokine Profile by ELISA in Patients with Echinococcal Cysts of the Liver: A Stage-Specific Approach to Assess Their Biological Activity
To investigate the usefulness of serum cytokine dosage in the clinical management of cystic echinococcosis (CE), we analyzed serum levels of Th1 and Th2 cytokines in patients with hepatic CE in different cyst stages, CE1-2 (active), CE3a-3b (transitional), and CE4-5 (inactive). Ex vivo assessment of...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/483935 |
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| author | Luca Piccoli Valeria Meroni Francesca Genco Francesca Tamarozzi Carmine Tinelli Carlo Filice Enrico Brunetti |
| author_facet | Luca Piccoli Valeria Meroni Francesca Genco Francesca Tamarozzi Carmine Tinelli Carlo Filice Enrico Brunetti |
| author_sort | Luca Piccoli |
| collection | DOAJ |
| description | To investigate the usefulness of serum cytokine dosage in the clinical management of cystic echinococcosis (CE), we analyzed serum levels of Th1 and Th2 cytokines in patients with hepatic CE in different cyst stages, CE1-2 (active), CE3a-3b (transitional), and CE4-5 (inactive). Ex vivo assessment of Th1 (IFN-γ) and Th2 (IL-4, IL-13, and IL-10) cytokines in sera was carried out using ELISA. IL-10 was undetectable in all serum samples of patients and controls, while a few sera contained measurable amounts of IFN-γ, IL-4, and IL-13. No statistically significant difference was found between the percentages of positive samples for each cytokine and the different groups analyzed (patients/controls, stage, number, location, and size of the cyst, serology, and sex of patients), with the exception of the association of IL-4 and IL-13 with the cyst stage. Overall, this investigation showed many limits of serum cytokine dosage as a marker of biological activity of echinococcal cysts. Because of low sensitivity and lack of specificity of this test, we believe that other ways to evaluate ex vivo biological activity of the cysts should be explored. |
| format | Article |
| id | doaj-art-c40a1202d7e347d99832ba828d9bffb1 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-c40a1202d7e347d99832ba828d9bffb12025-02-03T01:02:06ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/483935483935Serum Cytokine Profile by ELISA in Patients with Echinococcal Cysts of the Liver: A Stage-Specific Approach to Assess Their Biological ActivityLuca Piccoli0Valeria Meroni1Francesca Genco2Francesca Tamarozzi3Carmine Tinelli4Carlo Filice5Enrico Brunetti6Department of Infectious Diseases, IRCCS San Matteo Hospital Foundation, University of Pavia, and WHO Collaborating Centre for Clinical Management of Cystic Echinococcosis, Pavia 27100, ItalyLaboratory of Parasitology, Department of Infectious Diseases, IRCCS San Matteo Hospital Foundation, University of Pavia, Pavia 27100, ItalyLaboratory of Parasitology, Department of Infectious Diseases, IRCCS San Matteo Hospital Foundation, University of Pavia, Pavia 27100, ItalyDepartment of Infectious Diseases, IRCCS San Matteo Hospital Foundation, University of Pavia, and WHO Collaborating Centre for Clinical Management of Cystic Echinococcosis, Pavia 27100, ItalyClinical Epidemiology and Biometric Unit, IRCCS San Matteo Hospital Foundation, Pavia 27100, ItalyDepartment of Infectious Diseases, IRCCS San Matteo Hospital Foundation, University of Pavia, and WHO Collaborating Centre for Clinical Management of Cystic Echinococcosis, Pavia 27100, ItalyDepartment of Infectious Diseases, IRCCS San Matteo Hospital Foundation, University of Pavia, and WHO Collaborating Centre for Clinical Management of Cystic Echinococcosis, Pavia 27100, ItalyTo investigate the usefulness of serum cytokine dosage in the clinical management of cystic echinococcosis (CE), we analyzed serum levels of Th1 and Th2 cytokines in patients with hepatic CE in different cyst stages, CE1-2 (active), CE3a-3b (transitional), and CE4-5 (inactive). Ex vivo assessment of Th1 (IFN-γ) and Th2 (IL-4, IL-13, and IL-10) cytokines in sera was carried out using ELISA. IL-10 was undetectable in all serum samples of patients and controls, while a few sera contained measurable amounts of IFN-γ, IL-4, and IL-13. No statistically significant difference was found between the percentages of positive samples for each cytokine and the different groups analyzed (patients/controls, stage, number, location, and size of the cyst, serology, and sex of patients), with the exception of the association of IL-4 and IL-13 with the cyst stage. Overall, this investigation showed many limits of serum cytokine dosage as a marker of biological activity of echinococcal cysts. Because of low sensitivity and lack of specificity of this test, we believe that other ways to evaluate ex vivo biological activity of the cysts should be explored.http://dx.doi.org/10.1155/2012/483935 |
| spellingShingle | Luca Piccoli Valeria Meroni Francesca Genco Francesca Tamarozzi Carmine Tinelli Carlo Filice Enrico Brunetti Serum Cytokine Profile by ELISA in Patients with Echinococcal Cysts of the Liver: A Stage-Specific Approach to Assess Their Biological Activity Clinical and Developmental Immunology |
| title | Serum Cytokine Profile by ELISA in Patients with Echinococcal Cysts of the Liver: A Stage-Specific Approach to Assess Their Biological Activity |
| title_full | Serum Cytokine Profile by ELISA in Patients with Echinococcal Cysts of the Liver: A Stage-Specific Approach to Assess Their Biological Activity |
| title_fullStr | Serum Cytokine Profile by ELISA in Patients with Echinococcal Cysts of the Liver: A Stage-Specific Approach to Assess Their Biological Activity |
| title_full_unstemmed | Serum Cytokine Profile by ELISA in Patients with Echinococcal Cysts of the Liver: A Stage-Specific Approach to Assess Their Biological Activity |
| title_short | Serum Cytokine Profile by ELISA in Patients with Echinococcal Cysts of the Liver: A Stage-Specific Approach to Assess Their Biological Activity |
| title_sort | serum cytokine profile by elisa in patients with echinococcal cysts of the liver a stage specific approach to assess their biological activity |
| url | http://dx.doi.org/10.1155/2012/483935 |
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