A One-Step Reverse Transcription PCR Assay for the Detection of Transmissible Gastroenteritis Virus Genome in Pigs

A One-Step Reverse Transcription PCR Assay for the Detection of Transmissible Gastroenteritis Virus Genome in Pigs

The study focused on developing an indigenous, one-step reverse transcription (RT)-PCR assay for detecting the porcine transmissible gastroenteritis virus (TGEV) genome. In summary, a gene construct and two sets of primers were designed by aligning N gene sequences from various TGEV strains, which w...

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Bibliographic Details
Main Authors: Fateh Singh, Katherukamem Rajukumar, Dhanapal Senthilkumar, Govindarajulu Venkatesh, Gopal Sarkar, Jaswant Patel, Rohit Sahu, Nourin Khan, Atul Kumar Pateriya, Vijendra Pal Singh, Aniket Sanyal
Format: Article
Language:English
Published: Journal of Pure and Applied Microbiology 2025-03-01
Series:Journal of Pure and Applied Microbiology
Subjects:
diagnostic preparedness
disease monitoring
genomic detection
pigs
reverse transcription-pcr
transmissible gastroenteritis
tge virus
Online Access:https://microbiologyjournal.org/a-one-step-reverse-transcription-pcr-assay-for-the-detection-of-transmissible-gastroenteritis-virus-genome-in-pigs/
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https://microbiologyjournal.org/a-one-step-reverse-transcription-pcr-assay-for-the-detection-of-transmissible-gastroenteritis-virus-genome-in-pigs/

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