Study on the Correlation between Interleukin-27 and CXCL10 in Pulmonary Tuberculosis
Objective. To investigate the correlation between interleukin-27 and CXCL10 and other cytokines in pulmonary tuberculosis and to further explore the related miRNAs through bioinformatics. Methods. Collect the lesion tissue and peripheral blood of pulmonary tuberculosis patients and the peripheral bl...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/2932837 |
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| _version_ | 1832551213163347968 |
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| author | Jiahui Fan Yefeng Yang Liang Wang Xiaoqian Shang Li Zhang Hu Sun Yujie Ma Ying Li Jing Wang Xiumin Ma |
| author_facet | Jiahui Fan Yefeng Yang Liang Wang Xiaoqian Shang Li Zhang Hu Sun Yujie Ma Ying Li Jing Wang Xiumin Ma |
| author_sort | Jiahui Fan |
| collection | DOAJ |
| description | Objective. To investigate the correlation between interleukin-27 and CXCL10 and other cytokines in pulmonary tuberculosis and to further explore the related miRNAs through bioinformatics. Methods. Collect the lesion tissue and peripheral blood of pulmonary tuberculosis patients and the peripheral blood of healthy controls. Immunohistochemical staining and qRT-PCR were used to observe the expression of interleukin-27, CXCL9, CXCL10, and CXCL11. Then, predict the key miRNA, qRT-PCR was used to verify the expression of miRNA in the peripheral blood and evaluated the correlation between them. Results. Both immunohistochemical staining and qRT-PCR indicated that the expressions of IL-27, CXCL9, CXCL10, and CXCL11 were significantly increased in tuberculosis patients, and IL-27 was significantly correlated with CXCL10 (r=0.68). Key molecules such as has-let-7b-5p, has-miR-30a-3p, and has-miR-320b were screened out. Among them, has-let-7b-5p was significantly downregulated, and has-miR-30a-3p was significantly upregulated; they were related to interleukin-27 and CXCL10. Conclusion. Our data shows that interleukin-27 and CXCL10 are significantly related in pulmonary tuberculosis, and has-let-7b-5p and has-miR-30a-3p are also related to interleukin-27 and CXCL10. It laid the foundation for subsequently exploiting the potential biomarkers in tuberculosis disease. |
| format | Article |
| id | doaj-art-c376afcf4c4140eeb1d7d7fd97d35a60 |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-c376afcf4c4140eeb1d7d7fd97d35a602025-02-03T06:04:44ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/2932837Study on the Correlation between Interleukin-27 and CXCL10 in Pulmonary TuberculosisJiahui Fan0Yefeng Yang1Liang Wang2Xiaoqian Shang3Li Zhang4Hu Sun5Yujie Ma6Ying Li7Jing Wang8Xiumin Ma9State Key Laboratory of PathogenesisDepartment of Respiratory MedicineState Key Laboratory of PathogenesisState Key Laboratory of PathogenesisState Key Laboratory of PathogenesisState Key Laboratory of PathogenesisState Key Laboratory of PathogenesisState Key Laboratory of PathogenesisDepartment of Respiratory MedicineState Key Laboratory of PathogenesisObjective. To investigate the correlation between interleukin-27 and CXCL10 and other cytokines in pulmonary tuberculosis and to further explore the related miRNAs through bioinformatics. Methods. Collect the lesion tissue and peripheral blood of pulmonary tuberculosis patients and the peripheral blood of healthy controls. Immunohistochemical staining and qRT-PCR were used to observe the expression of interleukin-27, CXCL9, CXCL10, and CXCL11. Then, predict the key miRNA, qRT-PCR was used to verify the expression of miRNA in the peripheral blood and evaluated the correlation between them. Results. Both immunohistochemical staining and qRT-PCR indicated that the expressions of IL-27, CXCL9, CXCL10, and CXCL11 were significantly increased in tuberculosis patients, and IL-27 was significantly correlated with CXCL10 (r=0.68). Key molecules such as has-let-7b-5p, has-miR-30a-3p, and has-miR-320b were screened out. Among them, has-let-7b-5p was significantly downregulated, and has-miR-30a-3p was significantly upregulated; they were related to interleukin-27 and CXCL10. Conclusion. Our data shows that interleukin-27 and CXCL10 are significantly related in pulmonary tuberculosis, and has-let-7b-5p and has-miR-30a-3p are also related to interleukin-27 and CXCL10. It laid the foundation for subsequently exploiting the potential biomarkers in tuberculosis disease.http://dx.doi.org/10.1155/2022/2932837 |
| spellingShingle | Jiahui Fan Yefeng Yang Liang Wang Xiaoqian Shang Li Zhang Hu Sun Yujie Ma Ying Li Jing Wang Xiumin Ma Study on the Correlation between Interleukin-27 and CXCL10 in Pulmonary Tuberculosis Journal of Immunology Research |
| title | Study on the Correlation between Interleukin-27 and CXCL10 in Pulmonary Tuberculosis |
| title_full | Study on the Correlation between Interleukin-27 and CXCL10 in Pulmonary Tuberculosis |
| title_fullStr | Study on the Correlation between Interleukin-27 and CXCL10 in Pulmonary Tuberculosis |
| title_full_unstemmed | Study on the Correlation between Interleukin-27 and CXCL10 in Pulmonary Tuberculosis |
| title_short | Study on the Correlation between Interleukin-27 and CXCL10 in Pulmonary Tuberculosis |
| title_sort | study on the correlation between interleukin 27 and cxcl10 in pulmonary tuberculosis |
| url | http://dx.doi.org/10.1155/2022/2932837 |
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