Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs
Objectives. Endothelial cell injury is a critical pathological change during the development of atherosclerosis. Here, we explored the effect of omentin-1 on free fatty acid- (FFA-) induced endothelial cell injury. Methods. An FFA-induced endothelial cell injury model was established to investigate...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Cardiology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2020/3054379 |
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| author | Yubin Chen Fen Liu Fei Han Lizhi Lv Can-e Tang Fanyan Luo |
| author_facet | Yubin Chen Fen Liu Fei Han Lizhi Lv Can-e Tang Fanyan Luo |
| author_sort | Yubin Chen |
| collection | DOAJ |
| description | Objectives. Endothelial cell injury is a critical pathological change during the development of atherosclerosis. Here, we explored the effect of omentin-1 on free fatty acid- (FFA-) induced endothelial cell injury. Methods. An FFA-induced endothelial cell injury model was established to investigate the role of omentin-1 in this process. Cell proliferation was analyzed with the Cell Counting Kit assay and flow cytometry. Scratch and transwell assays were used to evaluate cell migration. Factors secreted by endothelial cells after injury were detected by western blotting, reverse-transcription quantitative polymerase chain reaction, and cellular fluorescence assay. Results. Omentin-1 rescued the FFA-induced impaired proliferation and migration capabilities of human umbilical vein endothelial cells (HUVECs). It decreased the number of THP-1 cells attached to HUVECs in response to injury and inhibited the FFA-induced proinflammatory state of HUVECs. Conclusion. Omentin-1 could partly ameliorate FFA-induced endothelial cell injury. |
| format | Article |
| id | doaj-art-c3355441f26443d99e23dba20050751c |
| institution | Kabale University |
| issn | 2090-8016 2090-0597 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cardiology Research and Practice |
| spelling | doaj-art-c3355441f26443d99e23dba20050751c2025-02-03T00:59:43ZengWileyCardiology Research and Practice2090-80162090-05972020-01-01202010.1155/2020/30543793054379Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECsYubin Chen0Fen Liu1Fei Han2Lizhi Lv3Can-e Tang4Fanyan Luo5Department of Cardiac Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, ChinaThe Institute of Medical Science Research, Xiangya Hospital, Central South University, Changsha 410008, Hunan, ChinaDepartment of Cardiac Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, ChinaDepartment of Cardiac Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, ChinaThe Institute of Medical Science Research, Xiangya Hospital, Central South University, Changsha 410008, Hunan, ChinaDepartment of Cardiac Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, ChinaObjectives. Endothelial cell injury is a critical pathological change during the development of atherosclerosis. Here, we explored the effect of omentin-1 on free fatty acid- (FFA-) induced endothelial cell injury. Methods. An FFA-induced endothelial cell injury model was established to investigate the role of omentin-1 in this process. Cell proliferation was analyzed with the Cell Counting Kit assay and flow cytometry. Scratch and transwell assays were used to evaluate cell migration. Factors secreted by endothelial cells after injury were detected by western blotting, reverse-transcription quantitative polymerase chain reaction, and cellular fluorescence assay. Results. Omentin-1 rescued the FFA-induced impaired proliferation and migration capabilities of human umbilical vein endothelial cells (HUVECs). It decreased the number of THP-1 cells attached to HUVECs in response to injury and inhibited the FFA-induced proinflammatory state of HUVECs. Conclusion. Omentin-1 could partly ameliorate FFA-induced endothelial cell injury.http://dx.doi.org/10.1155/2020/3054379 |
| spellingShingle | Yubin Chen Fen Liu Fei Han Lizhi Lv Can-e Tang Fanyan Luo Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs Cardiology Research and Practice |
| title | Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs |
| title_full | Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs |
| title_fullStr | Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs |
| title_full_unstemmed | Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs |
| title_short | Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs |
| title_sort | omentin 1 ameliorated free fatty acid induced impairment in proliferation migration and inflammatory states of huvecs |
| url | http://dx.doi.org/10.1155/2020/3054379 |
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