Rapid isothermal molecular tests to discriminate between Leishmania braziliensis and Leishmania infantum infections in dogs

Rapid isothermal molecular tests to discriminate between Leishmania braziliensis and Leishmania infantum infections in dogs

Abstract Background We standardized two recombinase polymerase amplification (RPA) assays coupled with lateral flow (LF) strips for the detection of Leishmania braziliensis and Leishmania infantum kinetoplast DNA (kDNA). Methods The RPA-LF assays were tested at different temperatures and reaction ti...

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Main Authors: Rafaela Lira Nogueira de Luna, Kamila Gaudêncio da Silva Sales, Lucas Lisboa Nunes Bonifácio, Luciana Aguiar Figueredo, Thomas R. Shelite, Fábio dos Santos Nogueira, Domenico Otranto, Filipe Dantas-Torres
Format: Article
Language:English
Published: BMC 2025-01-01
Series:Parasites & Vectors
Subjects:
Isothermal amplification
Leishmaniasis
Molecular diagnosis
Point-of-care
Recombinase
Online Access:https://doi.org/10.1186/s13071-024-06633-7
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https://doi.org/10.1186/s13071-024-06633-7

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