Regulation of Exogenous Strigolactone on Storage Substance Metabolism and Endogenous Hormone Levels in the Early Germination Stage of Rice Seeds Under Salt Stress
Salt stress inhibits rice seed germination. Strigolactone (GR24) plays a vital role in enhancing plant tolerance against salt stress. However, GR24’s impact on the metabolism of stored substances and endogenous hormones remains unclear. This study investigated the impact of exogenous GR24 on the met...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/14/1/22 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832589354461036544 |
|---|---|
| author | Jianqin Zhang Dianfeng Zheng Naijie Feng Aaqil Khan Rui Deng Jian Xiong Linchong Ding Zhiyuan Sun Jiahuan Li Xiaohui Yang Chen Wu |
| author_facet | Jianqin Zhang Dianfeng Zheng Naijie Feng Aaqil Khan Rui Deng Jian Xiong Linchong Ding Zhiyuan Sun Jiahuan Li Xiaohui Yang Chen Wu |
| author_sort | Jianqin Zhang |
| collection | DOAJ |
| description | Salt stress inhibits rice seed germination. Strigolactone (GR24) plays a vital role in enhancing plant tolerance against salt stress. However, GR24’s impact on the metabolism of stored substances and endogenous hormones remains unclear. This study investigated the impact of exogenous GR24 on the metabolism of stored substances and endogenous hormones during the early stages of rice seed germination under salt stress. The results showed that salt stress significantly reduced the germination rate, germination potential, germination index, radicle length, germ length, and fresh and dry weights of the radicle and germ under salt stress. Pre-treatment (1.2 μmol L<sup>−1</sup> GR24) significantly reduced the inhibition of salt stress on rice seed germination and seedling growth. GR24 promoted the decomposition of starch by enhancing the activities of α-amylase, β-amylase, and total amylase and improved the levels of soluble sugars and proteins and the conversion rate of substances under salt stress. GR24 effectively enhanced the activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX); increased ascorbic acid (ASA) and glutathione (GSH) levels; and reduced malondialdehyde (MDA) content. This reduced the oxidative damage of salt stress. Furthermore, GR24 significantly increased the contents of strigolactones (SLs), auxin (IAA), gibberellin (GA3), cytokinin (CTK) as well as IAA/ABA, CTK/ABA, GA/ABA, and SL/ABA ratios and reduced abscisic acid (ABA) levels. The current findings indicate that GR24 effectively mitigates the adverse impact salt stress by regulating antioxidant enzyme activity and endogenous hormone balance. |
| format | Article |
| id | doaj-art-c2c0401f8c1643b4ae5e1d0393e8001d |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-c2c0401f8c1643b4ae5e1d0393e8001d2025-01-24T13:19:10ZengMDPI AGAntioxidants2076-39212024-12-011412210.3390/antiox14010022Regulation of Exogenous Strigolactone on Storage Substance Metabolism and Endogenous Hormone Levels in the Early Germination Stage of Rice Seeds Under Salt StressJianqin Zhang0Dianfeng Zheng1Naijie Feng2Aaqil Khan3Rui Deng4Jian Xiong5Linchong Ding6Zhiyuan Sun7Jiahuan Li8Xiaohui Yang9Chen Wu10College of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaSalt stress inhibits rice seed germination. Strigolactone (GR24) plays a vital role in enhancing plant tolerance against salt stress. However, GR24’s impact on the metabolism of stored substances and endogenous hormones remains unclear. This study investigated the impact of exogenous GR24 on the metabolism of stored substances and endogenous hormones during the early stages of rice seed germination under salt stress. The results showed that salt stress significantly reduced the germination rate, germination potential, germination index, radicle length, germ length, and fresh and dry weights of the radicle and germ under salt stress. Pre-treatment (1.2 μmol L<sup>−1</sup> GR24) significantly reduced the inhibition of salt stress on rice seed germination and seedling growth. GR24 promoted the decomposition of starch by enhancing the activities of α-amylase, β-amylase, and total amylase and improved the levels of soluble sugars and proteins and the conversion rate of substances under salt stress. GR24 effectively enhanced the activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX); increased ascorbic acid (ASA) and glutathione (GSH) levels; and reduced malondialdehyde (MDA) content. This reduced the oxidative damage of salt stress. Furthermore, GR24 significantly increased the contents of strigolactones (SLs), auxin (IAA), gibberellin (GA3), cytokinin (CTK) as well as IAA/ABA, CTK/ABA, GA/ABA, and SL/ABA ratios and reduced abscisic acid (ABA) levels. The current findings indicate that GR24 effectively mitigates the adverse impact salt stress by regulating antioxidant enzyme activity and endogenous hormone balance.https://www.mdpi.com/2076-3921/14/1/22GR24riceseed germinationmetabolism of stored substancesendogenous hormones |
| spellingShingle | Jianqin Zhang Dianfeng Zheng Naijie Feng Aaqil Khan Rui Deng Jian Xiong Linchong Ding Zhiyuan Sun Jiahuan Li Xiaohui Yang Chen Wu Regulation of Exogenous Strigolactone on Storage Substance Metabolism and Endogenous Hormone Levels in the Early Germination Stage of Rice Seeds Under Salt Stress Antioxidants GR24 rice seed germination metabolism of stored substances endogenous hormones |
| title | Regulation of Exogenous Strigolactone on Storage Substance Metabolism and Endogenous Hormone Levels in the Early Germination Stage of Rice Seeds Under Salt Stress |
| title_full | Regulation of Exogenous Strigolactone on Storage Substance Metabolism and Endogenous Hormone Levels in the Early Germination Stage of Rice Seeds Under Salt Stress |
| title_fullStr | Regulation of Exogenous Strigolactone on Storage Substance Metabolism and Endogenous Hormone Levels in the Early Germination Stage of Rice Seeds Under Salt Stress |
| title_full_unstemmed | Regulation of Exogenous Strigolactone on Storage Substance Metabolism and Endogenous Hormone Levels in the Early Germination Stage of Rice Seeds Under Salt Stress |
| title_short | Regulation of Exogenous Strigolactone on Storage Substance Metabolism and Endogenous Hormone Levels in the Early Germination Stage of Rice Seeds Under Salt Stress |
| title_sort | regulation of exogenous strigolactone on storage substance metabolism and endogenous hormone levels in the early germination stage of rice seeds under salt stress |
| topic | GR24 rice seed germination metabolism of stored substances endogenous hormones |
| url | https://www.mdpi.com/2076-3921/14/1/22 |
| work_keys_str_mv | AT jianqinzhang regulationofexogenousstrigolactoneonstoragesubstancemetabolismandendogenoushormonelevelsintheearlygerminationstageofriceseedsundersaltstress AT dianfengzheng regulationofexogenousstrigolactoneonstoragesubstancemetabolismandendogenoushormonelevelsintheearlygerminationstageofriceseedsundersaltstress AT naijiefeng regulationofexogenousstrigolactoneonstoragesubstancemetabolismandendogenoushormonelevelsintheearlygerminationstageofriceseedsundersaltstress AT aaqilkhan regulationofexogenousstrigolactoneonstoragesubstancemetabolismandendogenoushormonelevelsintheearlygerminationstageofriceseedsundersaltstress AT ruideng regulationofexogenousstrigolactoneonstoragesubstancemetabolismandendogenoushormonelevelsintheearlygerminationstageofriceseedsundersaltstress AT jianxiong regulationofexogenousstrigolactoneonstoragesubstancemetabolismandendogenoushormonelevelsintheearlygerminationstageofriceseedsundersaltstress AT linchongding regulationofexogenousstrigolactoneonstoragesubstancemetabolismandendogenoushormonelevelsintheearlygerminationstageofriceseedsundersaltstress AT zhiyuansun regulationofexogenousstrigolactoneonstoragesubstancemetabolismandendogenoushormonelevelsintheearlygerminationstageofriceseedsundersaltstress AT jiahuanli regulationofexogenousstrigolactoneonstoragesubstancemetabolismandendogenoushormonelevelsintheearlygerminationstageofriceseedsundersaltstress AT xiaohuiyang regulationofexogenousstrigolactoneonstoragesubstancemetabolismandendogenoushormonelevelsintheearlygerminationstageofriceseedsundersaltstress AT chenwu regulationofexogenousstrigolactoneonstoragesubstancemetabolismandendogenoushormonelevelsintheearlygerminationstageofriceseedsundersaltstress |